Carrier-free highly drug-loaded biomimetic nanosuspensions encapsulated by cancer cell membrane based on homology and active targeting for the treatment of glioma DOI Creative Commons

Yueyue Fan,

Yuexin Cui,

Wenyan Hao

и другие.

Bioactive Materials, Год журнала: 2021, Номер 6(12), С. 4402 - 4414

Опубликована: Май 1, 2021

Nanosuspensions, as a new drug delivery system for insoluble drugs, are only composed of and small amount stabilizer, which is dispersed in an aqueous solution with high drug-loading, particle size, dispersion, large specific surface area. It can significantly improve the dissolution, bioavailability, efficacy drugs. In this study, paclitaxel nanosuspensions ((PTX)NS) were prepared by ultrasonic precipitation method, characteristics simple preparation easy repetition. With help homologous targeting mechanism, kind glioma C6 cancer cell membrane (CCM)-coated (PTX)NS was developed modified DWSW peptide to obtain DWSW-CCM-(PTX)NS functions BBB penetration tumor targeting. The results showed that could effectively camouflage so it not cleared immune cross blood-brain-barrier (BBB) selectively target tissues. Cell uptake experiments vivo imaging confirmed cells distribution intracranial gliomas increased. Cytotoxicity test anti-glioma studies inhibit growth prolong survival time glioma-bearing mice. Finally, coating endowed biological properties adhesion escape. This study provides integrated improving demonstrates encouraging potential biomimetic applicable therapy.

Язык: Английский

Cell membrane coating integrity affects the internalization mechanism of biomimetic nanoparticles DOI Creative Commons
Lizhi Liu, Xuan Bai, Maria‐Viola Martikainen

и другие.

Nature Communications, Год журнала: 2021, Номер 12(1)

Опубликована: Сен. 30, 2021

Cell membrane coated nanoparticles (NPs) have recently been recognized as attractive nanomedical tools because of their unique properties such immune escape, long blood circulation time, specific molecular recognition and cell targeting. However, the integrity coating on NPs, a key metrics related to quality these biomimetic-systems resulting biomedical function, has remained largely unexplored. Here, we report fluorescence quenching assay probe coating. In contradiction common assumption perfect coating, uncover that up 90% biomimetic NPs are only partially coated. Using in vitro homologous targeting studies, demonstrate could still be internalized by target cells. By combining simulations with experimental analysis, further identify an endocytic entry mechanism for NPs. We unravel high degree (≥50%) enter cells individually, whereas low (<50%) need aggregate together before internalization. This quantitative method fundamental understanding how will enhance rational designing nanosystems pave way more effective cancer nanomedicine.

Язык: Английский

Процитировано

264

Recent progress in targeted delivery vectors based on biomimetic nanoparticles DOI Creative Commons
Li Chen, Weiqi Hong, Wenyan Ren

и другие.

Signal Transduction and Targeted Therapy, Год журнала: 2021, Номер 6(1)

Опубликована: Июнь 7, 2021

Abstract Over the past decades, great interest has been given to biomimetic nanoparticles (BNPs) since rise of targeted drug delivery systems and nanotechnology. Biological vectors including cell membranes, extracellular vesicles (EVs), viruses are considered promising candidates for owing their biocompatibility biodegradability. BNPs, integration biological functional agents, anticipated load cargos or camouflage synthetic achieve delivery. Despite excellent intrinsic properties, natural deliberately modified endow multiple functions such as good permeability, improved loading capability, high specificity. Through structural modification transformation vectors, they pervasively utilized more effective vehicles that can deliver contrast chemotherapy drugs, nucleic acids, genes target sites refractory disease therapy. This review summarizes recent advances in based on EVs, viruses, highlighting potential applications BNPs fields biomedical imaging therapy industry, well discussing possibility clinical translation exploitation trend these BNPs.

Язык: Английский

Процитировано

231

Cell membrane coated nanocarriers - an efficient biomimetic platform for targeted therapy DOI

Pratigyan Dash,

Anna Maria Piras, Mamoni Dash

и другие.

Journal of Controlled Release, Год журнала: 2020, Номер 327, С. 546 - 570

Опубликована: Сен. 8, 2020

Язык: Английский

Процитировано

194

Harnessing Endogenous Stimuli for Responsive Materials in Theranostics DOI Creative Commons
Alexander Cook, Paolo Decuzzi

ACS Nano, Год журнала: 2021, Номер 15(2), С. 2068 - 2098

Опубликована: Фев. 8, 2021

Materials that respond to endogenous stimuli are being leveraged enhance spatiotemporal control in a range of biomedical applications from drug delivery diagnostic tools. The design materials undergo morphological or chemical changes response specific biological cues pathologies will be an important area research for improving efficacies existing therapies and imaging agents, while also promising developing personalized theranostic systems. Internal stimuli-responsive systems can engineered across length scales nanometers macroscopic signals such as enzymes, pH, glucose, ATP, hypoxia, redox signals, nucleic acids by incorporating synthetic bio-inspired moieties natural building blocks. This Review summarize mechanisms fabrication strategies used internal with focus on broad pathologies, including cancer, diabetes, vascular disorders, inflammation, microbial infections. We discuss observed challenges, future directions, clinical translation aspects these responsive materials.

Язык: Английский

Процитировано

176

Advances in nanomaterial-based targeted drug delivery systems DOI Creative Commons

Xiaoxiao Cheng,

Qirong Xie,

Yang Sun

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2023, Номер 11

Опубликована: Апрель 13, 2023

Nanomaterial-based drug delivery systems (NBDDS) are widely used to improve the safety and therapeutic efficacy of encapsulated drugs due their unique physicochemical biological properties. By combining with nanoparticles using rational targeting pathways, nano-targeted were created overcome main drawbacks conventional treatment, including insufficient stability solubility, lack transmembrane transport, short circulation time, undesirable toxic effects. Herein, we reviewed recent developments in different design strategies approaches employing various nanomaterial-based systems. We also discussed challenges perspectives smart precisely intravascular extravascular diseases.

Язык: Английский

Процитировано

174

Cell membrane-coated nanoparticles: a novel multifunctional biomimetic drug delivery system DOI Open Access
Hui Liu, Yuyan Su,

Xinchi Jiang

и другие.

Drug Delivery and Translational Research, Год журнала: 2022, Номер 13(3), С. 716 - 737

Опубликована: Ноя. 22, 2022

Язык: Английский

Процитировано

122

Cell membrane-derived vesicles for delivery of therapeutic agents DOI Creative Commons
Quoc‐Viet Le, Jaiwoo Lee,

Hobin Lee

и другие.

Acta Pharmaceutica Sinica B, Год журнала: 2021, Номер 11(8), С. 2096 - 2113

Опубликована: Фев. 2, 2021

Cell membranes have recently emerged as a new source of materials for molecular delivery systems. been extruded or sonicated to make nanoscale vesicles. Unlike synthetic lipid polymeric nanoparticles, cell membrane-derived vesicles unique multicomponent feature, comprising lipids, proteins, and carbohydrates. Because contain the intrinsic functionalities signaling networks their parent cells, they can overcome various obstacles encountered

Язык: Английский

Процитировано

121

Brain Co‐Delivery of Temozolomide and Cisplatin for Combinatorial Glioblastoma Chemotherapy DOI
Yan Zou,

Yibin Wang,

Sen Xu

и другие.

Advanced Materials, Год журнала: 2022, Номер 34(33)

Опубликована: Июнь 23, 2022

Glioblastoma (GBM) is an intractable malignancy with high recurrence and mortality. Combinatorial therapy based on temozolomide (TMZ) cisplatin (CDDP) shows promising potential for GBM in clinical trials. However, significant challenges include limited blood-brain-barrier (BBB) penetration, poor targeting of tissue/cells, systemic side effects, which hinder its efficacy therapy. To surmount these challenges, new GBM-cell membrane camouflaged pH-sensitive biomimetic nanoparticles (MNPs) inspired by the fact that cancer cells readily pass BBB localize homologous cells, are developed. This study's results show MNPs can efficiently co-load TMZ CDDP, transport across to specifically target GBM. Incorporation polymer then allows controlled release drug cargos at sites combination Mice bearing orthotopic U87MG or drug-resistant U251R tumor treated MNPs@TMZ+CDDP a potent anti-GBM effect, greatly extending survival time relative mice receiving single-drug loaded nanoparticles. No obvious effects apparent histological analyses blood routine studies. Considering results, nanoparticle formulation overcomes multiple currently limiting combined CDDP appears be strategy future combinatorial chemotherapy.

Язык: Английский

Процитировано

121

Advances in Drug Delivery Systems Based on Red Blood Cells and Their Membrane-Derived Nanoparticles DOI
Phuong H. D. Nguyen,

Migara Kavishka Jayasinghe,

Anh Hong Le

и другие.

ACS Nano, Год журнала: 2023, Номер 17(6), С. 5187 - 5210

Опубликована: Март 10, 2023

Red blood cells (RBCs) and RBC membrane-derived nanoparticles have been historically developed as bioinspired drug delivery systems to combat the issues of premature clearance, toxicity, immunogenicity synthetic nanocarriers. RBC-based possess characteristics including biocompatibility, biodegradability, long circulation time, which make them suited for systemic administration. Therefore, they employed in designing optimal formulations various preclinical models clinical trials treat a wide range diseases. In this review, we provide an overview biology, synthesis, characterization based on RBCs their membrane whole RBCs, membrane-camouflaged nanoparticles, RBC-derived extracellular vesicles, hitchhiking. We also highlight conventional latest engineering strategies, along with therapeutic modalities, enhanced precision effectiveness delivery. Additionally, focus current state applications translation carriers, well discussing opportunities challenges associated these systems.

Язык: Английский

Процитировано

112

Systematic design of cell membrane coating to improve tumor targeting of nanoparticles DOI Creative Commons
Lizhi Liu, Dingyi Pan, Sheng Chen

и другие.

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Окт. 19, 2022

Cell membrane (CM) coating technology is increasingly being applied in nanomedicine, but the entire procedure including adsorption, rupture, and fusion not completely understood. Previously, we showed that majority of biomimetic nanoparticles (NPs) were only partially coated, mechanism underlying this partial remains unclear, which hinders further improvement technique. Here, show an intermediate state due to adsorption CM fragments or vesicles, latter could eventually be ruptured under external force. Such difficult self-repair achieve full limited fluidity. Building on our understanding detailed process, develop a general approach for fixing coating: phospholipid introduced as helper increase fluidity, promoting final lipid patches. The NPs coated with have high ratio (~23%) exhibit enhanced tumor targeting ability comparison traditionally (full ~6%). Our results provide mechanistic basis towards enhancing accumulation.

Язык: Английский

Процитировано

91