Biomaterials, Год журнала: 2024, Номер 315, С. 122895 - 122895
Опубликована: Окт. 19, 2024
Язык: Английский
Journal of Tissue Engineering, Год журнала: 2021, Номер 12
Опубликована: Янв. 1, 2021
Liver failure is a lethal condition with hepatocellular dysfunction, and liver transplantation presently the only effective treatment. However, due to limited availability of donors potential immune rejection, novel therapeutic strategies are actively sought restore normal hepatic architectures functions, especially for livers inherited metabolic dysfunctions or chronic diseases. Although conventional cell therapy has shown promising results, direct infusion hepatocytes hampered by hepatocyte sources, poor viability, engraftment. Hence, this review mainly highlights role stem cells progenitors as alternative source summarizes approaches based on tissue engineering improve delivery efficiency cells. Particularly, underlying mechanisms using discussed in two main aspects: paracrine effect differentiation. Moreover, tissue-engineering aggregates decellularized scaffolds bioengineering functional constructs compared terms replicate physiological structures. In end, potentially strategy combining premium advantages proposed future direction regeneration.
Язык: Английский
Процитировано
47
Advanced Science, Год журнала: 2021, Номер 9(3)
Опубликована: Ноя. 21, 2021
The use of engineered scaffolds or stem cells is investigated widely in the repair injured musculoskeletal tissue. However, combined regeneration hierarchical osteochondral tissue remains a challenge due to delamination between cartilage and subchondral bone difficulty spatial control over differentiation transplanted cells. Here, two types composite spheroids are prepared using adipose-derived (hADSCs) nanofibers coated with either transforming growth factor-β3 morphogenetic factor-2 for chondrogenesis osteogenesis, respectively. Each type spheroid then cultured within 3D-printed microchamber spatially arranged manner recapitulate bilayer structure presence inductive factors regionally modulates vitro chondrogenic osteogenic hADSCs biphasic construct without dedifferentiation. Furthermore, from each proliferate sprout successfully connect layers mimicking interface apertures. In vivo transplantation onto femoral trochlear groove defect rabbit knee joint results 21.2 ± 2.8% volume/total volume score 25.0 3.7. present approach can be an effective therapeutic platform engineer complex
Язык: Английский
Процитировано
45
Nano Convergence, Год журнала: 2022, Номер 9(1)
Опубликована: Дек. 19, 2022
Human mesenchymal stem cells (hMSCs)-derived extracellular vesicles (EVs) have been known to possess the features of origin cell with nano size and shown therapeutic potentials for regenerative medicine in recent studies as alternatives cell-based therapies. However, extremely low production yield, unknown effects derived from serum impurities, relatively bioactivities on doses must be overcome translational applications. As several reports demonstrated tunability secretion EVs, herein, we introduced three-dimensional (3D) culture priming approaches MSCs serum-free chemically defined media exclude side serum-derived impurities. Aggregates (spheroids) 3D dramatically enhanced EVs about 6.7 times more than two-dimensional (2D) culture, altered surface compositions. Further modulation combination TNF-α IFN-γ (TI) facilitated 1.4 without (9.4 2D priming), related tissue regenerations. Interestingly, unlike changing TI internal cytokines MSC-derived EVs. Through simulating characteristics bioinformatics analysis, regeneration-relative properties such angiogenesis, wound healing, anti-inflammation, anti-apoptosis, anti-fibrosis, three different types were comparatively analyzed using assays. The present study that a combinatory strategy, cultures media, provided optimum environments maximize regeneration-related impurities future
Язык: Английский
Процитировано
33
ACS Applied Nano Materials, Год журнала: 2023, Номер 6(15), С. 13792 - 13823
Опубликована: Июль 3, 2023
Nanomaterials that can mimic the activity of various enzymes have been studied extensively in biomedical field recent years. Nanozymes offer better stability and economic value comparison to natural enzymes. Additionally, nanozymes characteristics nanomaterials, such as photothermal properties designability, therefore considerable potential. The cellular microenvironment provides basis for maintaining normal cell growth function. Increasing evidence shows imbalances homeostasis constitute a major factor development many diseases. received attention years regulators microenvironment. In this Review, research on use remodel disease is discussed. First, definition, classification, enzyme-like activities are introduced; several microenvironments summarized including tumor, inflammatory, trauma microenvironments. role main strategies constructing intelligent responsive nanozyme systems specific applications remodeling diseased highlighted. Finally, therapeutic prospected. Nanozyme expected become strategy or an adjunct prevention treatment related
Язык: Английский
Процитировано
18
Advanced Science, Год журнала: 2024, Номер 11(31)
Опубликована: Июнь 14, 2024
Abstract Liver fibrosis is a chronic pathological condition lacking specific clinical treatments. Stem cells, with notable potential in regenerative medicine, offer promise treating liver fibrosis. However, stem cell therapy hindered by immunological rejection, carcinogenesis risk, efficacy variation, and high cost. secretome‐based cell‐free offers solutions to address these challenges, but it limited low delivery efficiency rapid clearance. Herein, an innovative approach for situ implantation of smart microneedle (MN) arrays enabling precisely controlled multiple therapeutic agents directly into fibrotic tissues developed. By integrating platinum‐based nanocatalytic combination therapy, the MN can deactivate hepatic stellate cells. Moreover, they promote excessive extracellular matrix degradation more than 75%, approaching normal levels. Additionally, provide hepatocyte protection while reducing inflammation levels ≈70–90%. They also exhibit remarkable capability scavenging almost 100% reactive oxygen species alleviating hypoxia. Ultimately, this treatment strategy effectively restrain progression. The comprehensive vitro vivo experiments, supplemented proteome transcriptome analyses, substantiate effectiveness fibrosis, holding immense applications.
Язык: Английский
Процитировано
7
Advanced Healthcare Materials, Год журнала: 2024, Номер unknown
Опубликована: Июль 17, 2024
Nanozymes, with their versatile composition and structural adaptability, present distinct advantages over natural enzymes including heightened stability, customizable catalytic activity, cost-effectiveness, simplified synthesis process, making them as promising alternatives in various applications. Recent advancements nanozyme research have shifted focus from serendipitous discovery toward a more systematic approach, leveraging machine learning, theoretical calculations, mechanistic explorations to engineer nanomaterial structures tailored functions. Despite its pivotal role, electron transfer, fundamental process catalysis, has often been overlooked previous reviews. This review comprehensively summarizes recent strategies for modulating transfer processes fine-tune the activity specificity of nanozymes, electron-hole separation carrier transfer. Furthermore, bioapplications these engineered antimicrobial treatments, cancer therapy, biosensing are also introduced. Ultimately, this aims offer invaluable insights design nanozymes enhanced performance, thereby advancing field research.
Язык: Английский
Процитировано
7
Nano Today, Год журнала: 2021, Номер 39, С. 101227 - 101227
Опубликована: Июнь 22, 2021
Язык: Английский
Процитировано
39
Burns & Trauma, Год журнала: 2022, Номер 10
Опубликована: Янв. 1, 2022
Abstract Adipose-derived stem cells (ADSCs) have promising applications in tissue regeneration. Currently, there are only a few ADSC products that been approved for clinical use. The application of ADSCs still faces many challenges. Here, we review emerging strategies to improve the therapeutic efficacy First, great quantity is often needed cell therapies, which requires advanced expansion technologies. In addition cell-derived also required development ‘cell-free’ therapies overcome drawbacks cell-based therapies. Second, it necessary strengthen regenerative functions ADSCs, including viability, differentiation and paracrine ability, repair regeneration different physiological pathophysiological conditions. Third, poor delivery efficiency restricts effect ADSCs. Effective methods include alleviating harsh microenvironments, enhancing targeting ability prolonging retention. Moreover, point out some critical issues about sources, effectiveness safety With these ADSC-based treatment holds promise
Язык: Английский
Процитировано
27
Theranostics, Год журнала: 2022, Номер 12(14), С. 6223 - 6241
Опубликована: Янв. 1, 2022
Ischemic diseases, the leading cause of disability and death, are caused by restriction or blockage blood flow in specific tissues, including ischemic cardiac, cerebrovascular peripheral vascular diseases. The regeneration functional vasculature network tissues is essential for treatment Direct delivery pro-angiogenesis factors, such as VEGF, has demonstrated effectiveness disease therapy but suffering from several obstacles, low efficacy sites uncontrolled modulation. In this review, we summarize molecular mechanisms inducing regeneration, providing guidance designing desired nanomedicines. We also introduce various nanomedicines to passive active targeting manner. To achieve efficient highlight targeted controllable modulation microenvironment using
Язык: Английский
Процитировано
25
Small, Год журнала: 2022, Номер 18(31)
Опубликована: Июль 7, 2022
Abstract Asherman's syndrome (AS) occurs as a consequence of severe damage to the endometrial basalis, usually leading menstrual abnormalities, infertility, and recurrent miscarriage in women. Currently, human endometrium‐derived adventitial cells (En‐ADVs) are considered ideal seed with high pluripotency for regenerative medicine. However, critical issues such noninvasive repair tissues, targeting native stem cells, continuous action injured sites not well resolved. Herein, En‐ADV spheroid‐loaded hierarchical microneedles (MN/En‐ADV) situ intrauterine developed. The flexible fabricated gelatin methacryloyl lactoferrin, imparting characteristics rapid degradation antimicrobial activity. Benefiting from an array microwells on microneedles, En‐ADVs can rapidly form 3D cell spheroids, which display higher potential proliferation, differentiation, migration than dissociated cells. With application MN/En‐ADV, repaired uteri show well‐defined myometrial regeneration, angiogenesis, increase receptivity rat AS model. Notably, embryos able implant reconstructed remain viable, indicating that this system promotes restoration both normal morphology reproductive function uterus. It is anticipated multifunctional MN/En‐ADV be candidate versatile tissue regeneration.
Язык: Английский
Процитировано
24