Unique transcriptional signatures of sleep loss across independently evolved cavefish populations

Journal of Experimental Zoology Part B Molecular and Developmental Evolution, Год журнала: 2020, Номер 334(7-8), С. 497 - 510

Опубликована: Апрель 29, 2020

Abstract Animals respond to sleep loss with compensatory rebound sleep, and this is thought be critical for the maintenance of physiological homeostasis. Sleep duration varies dramatically across animal species, but it not known whether evolutionary differences in are associated The Mexican cavefish, Astyanax mexicanus , has emerged as a powerful model studying evolution sleep. While eyed surface populations A. approximately 8 hr each day, multiple blind cavefish have converged on patterns that total little 2 providing opportunity examine accompanied by changes Here, we behavioral molecular response deprivation four independent . Our analysis indicates fish all three display robust recovery during day following nighttime deprivation, suggesting homeostasis remains intact cavefish. We profiled transcriptome‐wide number differentially expressed genes was greater population, population exhibited highest uniquely than any other population. Strikingly, majority unique individual cave populations, expression responses independently evolved populations. Together, these findings suggest despite dramatic reduction overall duration.

Язык: Английский

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Exploring life-long tissue homeostasis through lineage tracing and cell transplantation

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Май 1, 2023

Abstract Aging is accompanied by a progressive loss of tissue homeostasis, including declining stem-cell function and increased cancer susceptibility. The naturally short-lived African turquoise killifish has emerged as powerful system for investigating vertebrate aging. However, critical mass advanced genetic tools mechanistic studies been largely missing. Here, we develop the Killibow , multispectral transgenic line life-long lineage tracing, an immunocompromised rag2 mutant transplantation studies, mutants genomic instability (i.e. atm tp53 ). We performed series experiments using this platform, tracing following germline transplantation, identifying occurring age-related melanoma engraftment into mutants. Exploring tumor dynamics reveals intriguing interplay between adaptive immunity, evolutionarily conserved decline in immune functions. Together, toolkit streamlines investigation molecular mechanisms underlying homeostasis during aging disease.

Язык: Английский

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Characterizing the genetic basis of trait evolution in the Mexican cavefish

Evolution & Development, Год журнала: 2022, Номер 24(5), С. 131 - 144

Опубликована: Авг. 4, 2022

Evolution in response to a change ecology often coincides with various morphological, physiological, and behavioral traits. For most organisms little is known about the genetic functional relationship between evolutionarily derived traits, representing critical gap our understanding of adaptation. The Mexican tetra, Astyanax mexicanus, consists largely independent populations fish that inhabit at least 30 caves Northeast Mexico, surface population, rivers Mexico Southern Texas. recent application molecular approaches combined phenotyping have established A. mexicanus as model for studying evolution complex Cave are interfertile evolved numerous traits including eye degeneration, insomnia, albinism, enhanced mechanosensory function. interfertility different from same species provides unique opportunity define assess co-evolution morphological one another. To relationships we developed pipeline test individual multiple This confirmed differences locomotor activity, prey capture, startle reflex cavefish populations. measure F2 hybrid were characterized behavior, prey-capture reflex, attributes. Analysis revealed an association body length slower escape suggesting trade-off increased size predator avoidance cavefish. Overall, there few associations or changes underlie measured Taken together, this approach novel system identify underpinnings naturally occurring variation

Язык: Английский

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Covariation of brain and skull shapes as a model to understand the role of crosstalk in development and evolution

Evolution & Development, Год журнала: 2022, Номер 25(1), С. 85 - 102

Опубликована: Ноя. 14, 2022

Abstract Covariation among discrete phenotypes can arise due to selection for shared functions, and/or genetic and developmental underpinnings. The consequences of such phenotypic integration are far‐reaching act either facilitate or limit morphological variation. vertebrate brain is known as an “organizer” craniofacial development, secreting morphogens that affect the shape growing neurocranium, consistent with roles pleiotropy in brain–neurocranium covariation. Here, we test this hypothesis cichlid fishes by first examining degree between neurocranium using three‐dimensional geometric morphometrics F 5 hybrid population, then genetically mapping trait covariation quantitative loci (QTL) analysis. We observe associations a pattern holds even when assess constituent parts neurocranium: rostrum braincase. also recover robust signals both hard‐ soft‐tissue traits identify genomic region where QTL braincase overlap, implicating role patterning Fine overlapping identifies candidate gene, notch1a , which be involved skeletal neural tissues during development. Taken together, these data offer covariation, well potential mechanism behavioral shifts may simultaneously drive rapid change neuroanatomy morphology.

Язык: Английский

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Evolution of left–right asymmetry in the sensory system and foraging behavior during adaptation to food-sparse cave environments

BMC Biology, Год журнала: 2022, Номер 20(1)

Опубликована: Дек. 27, 2022

Laterality in relation to behavior and sensory systems is found commonly a variety of animal taxa. Despite the advantages conferred by laterality (e.g., startle response complex motor activities), little known about evolution its plasticity ecological demands. In present study, comparative study model, Mexican tetra (Astyanax mexicanus), composed two morphotypes, i.e., riverine surface fish cave-dwelling cavefish, was used address relationship between environment laterality.

Язык: Английский

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