Spatially resolved transcriptomics: a comprehensive review of their technological advances, applications, and challenges

Journal of genetics and genomics/Journal of Genetics and Genomics, Год журнала: 2023, Номер 50(9), С. 625 - 640

Опубликована: Март 27, 2023

The ability to explore life kingdoms is largely driven by innovations and breakthroughs in technology, from the invention of microscope 350 years ago recent emergence single-cell sequencing, which scientific community has been able visualize at an unprecedented resolution. Most recently, Spatially Resolved Transcriptomics (SRT) technologies have filled gap probing spatial or even three-dimensional organization molecular foundation behind mysteries life, including origin different cellular populations developed totipotent cells human diseases. In this review, we introduce progress challenges on SRT perspectives bioinformatic tools, as well representative applications. With currently fast-moving promising results early adopted research projects, can foresee bright future such new tools understanding most profound analytical level.

Язык: Английский

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Enzyme Core Spherical Nucleic Acid That Enables Enhanced Cuproptosis and Antitumor Immune Response through Alleviating Tumor Hypoxia

Journal of the American Chemical Society, Год журнала: 2024, Номер 146(20), С. 13805 - 13816

Опубликована: Март 29, 2024

Cuproptosis, a copper-dependent cell death process, has been confirmed to further activate the immune response and mediate resistance. However, hypoxic tumor microenvironment hampers cuproptosis sensitivity suppresses body's antitumor response. Herein, we have successfully immobilized functionalized catalase (CAT) with long single-stranded DNA containing polyvalent CpG sequences through rolling circle amplification (RCA) techniques, obtaining an enzyme-cored spherical nucleic acid nanoplatform (CAT-ecSNA-Cu) deliver copper ions for cuproptosis. The presence of long-stranded DNA-protected CAT enhances mitochondrial respiration by catalyzing conversion H2O2 O2, thereby sensitizing Meanwhile, increased oxygenation expression hypoxia-inducible factor-1 (HIF-1) protein, resulting in alleviation immunosuppressive microenvironment. Of note, induces immunogenic (ICD), which facilitates dendritic (DC) maturation antigen presentation polyCpG-supported Toll-like receptor 9 (TLR9) activation. Furthermore, cuproptosis-induced PD-L1 upregulation cells complements checkpoint blockers (αPD-L1), enhancing immunity. strategy cuproptosis-mediated responses alleviating hypoxia effectively promotes activation proliferation effector T cells, ultimately leading long-term immunity against cancer.

Язык: Английский

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Spatiotemporal omics for biology and medicine

Cell, Год журнала: 2024, Номер 187(17), С. 4488 - 4519

Опубликована: Авг. 1, 2024

Язык: Английский

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Nanotechnology meets glioblastoma multiforme: Emerging therapeutic strategies

Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Год журнала: 2022, Номер 15(1)

Опубликована: Авг. 12, 2022

Glioblastoma multiforme (GBM) represents the most common and fatal form of primary invasive brain tumors as it affects a great number patients each year has median overall survival approximately 14.6 months after diagnosis. Despite intensive treatment, almost all with GBM experience recurrence, their 5-year rate is 5%. At present, main clinical treatment strategy includes surgical resection, radiotherapy, chemotherapy. However, tumor heterogeneity, blood-brain barrier, glioma stem cells, DNA damage repair mechanisms hinder efficient treatment. The emergence nanometer-scale diagnostic therapeutic approaches in cancer medicine due to establishment nanotechnology provides novel promising tools that will allow us overcome these difficulties. This review summarizes application recent progress nanotechnology-based monotherapies (e.g., chemotherapy) combination strategies (chemotherapy-based combined therapy) for describes synergistic enhancement between therapies well current standard therapy its deficiencies. These can reduce individual drug-related toxicities significantly enhance efficiency have recently undergone rapid development. underlying different photodynamic chemodynamic been systematically summarized here an attempt developments identify directions future research. clinically significant insights GBM, which importance. article categorized under: Therapeutic Approaches Drug Discovery > Nanomedicine Oncologic Disease Diagnostic Tools In Vivo Nanodiagnostics Imaging.

Язык: Английский

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Metal‐DNA Nanocomplexes Enhance Chemo‐dynamic Therapy by Inhibiting Autophagy‐Mediated Resistance

Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(50)

Опубликована: Ноя. 3, 2023

Chemo-dynamic therapy (CDT) based on the Fenton or Fenton-like reaction has emerged as a promising approach for cancer treatment. However, autophagy-mediated self-protection mechanisms of cells pose significant challenge to efficacy CDT. Herein, we developed metal-DNA nanocomplexes (DACs-Mn) enhance CDT via DNAzyme inhibition autophagy. Specifically, Mn-based catalyst in DACs-Mn was used generate highly hydroxyl radicals (⋅OH) that kill cells, while ATG5 incorporated into inhibited expression autophagy-associated proteins, thereby improving By disrupting self-protective pathway under severe oxidative stress, this novel found synergistically both vitro and vivo models, effectively amplifying tumor-specific damage. Notably, Metal-DNA can also induce immunogenic cell death (ICD), inhibiting tumor metastasis. bilateral model mice, combined autophagy followed by immune checkpoint blockade shown potential effective treatment modality primary metastatic tumors.

Язык: Английский

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Emerging Trends in DNA Nanotechnology-Enabled Cell Surface Engineering

JACS Au, Год журнала: 2025, Номер 5(2), С. 550 - 570

Опубликована: Фев. 6, 2025

Cell surface engineering is a rapidly advancing field, pivotal for understanding cellular physiology and driving innovations in biomedical applications. In this regard, DNA nanotechnology offers unprecedented potential precisely manipulating functionalizing cell surfaces by virtue of its inherent programmability versatile functionalities. Herein, Perspective provides comprehensive overview emerging trends engineering, focusing on key nanostructure-based tools, their roles regulating physiological processes, We first discuss the strategies integrating molecules onto surfaces, including attachment oligonucleotides higher-order nanostructure. Second, we summarize impact DNA-based various such as membrane protein degradation, signaling transduction, intercellular communication, construction artificial components. Third, highlight applications DNA-engineered targeted therapies cancer inflammation, well capture/protection diagnostic detection. Finally, address challenges future directions nanotechnology-based engineering. This aims to provide valuable insights rational design contributing development precise personalized medicine.

Язык: Английский

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Advances in Spatial Omics Technologies

Small Methods, Год журнала: 2025, Номер unknown

Опубликована: Март 18, 2025

Abstract Rapidly developing spatial omics technologies provide us with new approaches to deeply understanding the diversity and functions of cell types within organisms. Unlike traditional approaches, enable researchers dissect complex relationships between tissue structure function at cellular or even subcellular level. The application provides perspectives on key biological processes such as nervous system development, organ tumor microenvironment. This review focuses advancements strategies technologies, summarizes their applications in biomedical research, highlights power advancing life sciences related development disease.

Язык: Английский

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Ultrasensitive Hybridization Chain Reaction-Assisted Multisite Exonuclease III Amplification Strategy Combined with a Direct Quantitative Fluorescence Lateral Flow Technique for Multiple Bacterial 16S rRNA Detection

Analytical Chemistry, Год журнала: 2023, Номер 95(13), С. 5807 - 5814

Опубликована: Март 22, 2023

Accurate and in-time detection of bacteria conduces to preventing their rapid spread around the environment, while a nucleic acid test (NAT) is powerful tool for early diagnosis pathogens. Herein, we propose hybridization chain reaction (HCR)-mediated multisite exonuclease III (Exo-III) amplification strategy (HCR/Exo-III amplifier) achieve one-pot ultrasensitive isothermal bacterial 16S rRNA portable fluorescence device (PFD) directly read signals in lateral flow assay (LFA). In detail, target-initiated HCR products present multiple binding sites triggering Exo-III amplifier that produces numerous target amplicons. Following that, amplicons travel up on strip bridge between DNA-CdTe/CdS probes capture DNA form positive line. After inserted into PFD accomplish signal reading. The constructed HCR/Exo-III amplifier-based PFD-LFA implemented simultaneous specific three with limit few tenths fM synthetic fragments dozens CFU/mL Staphylococcus aureus, Listeria monocytogenes, Salmonella typhimurium pure cultures. sensing platform features amplification, convenient operation, good economy, displaying great potential on-site testing toward analytes.

Язык: Английский

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Framework Nucleic Acids Combined with 3D Hybridization Chain Reaction Amplifiers for Monitoring Multiple Human Tear Cytokines

Advanced Materials, Год журнала: 2024, Номер 36(26)

Опубликована: Март 16, 2024

Abstract Existing tear sensors are difficult to perform multiplexed assays due the minute amounts of biomolecules in tears and tiny volume tears. Herein, authors leverage DNA tetrahedral frameworks (DTFs) modified on wireless portable electrodes effectively capture 3D hybridization chain reaction (HCR) amplifiers for automatic sensitive monitoring multiple cytokines human The developed allow determination various dry eye syndrome (DES)‐associated with limit detection down 0.1 pg mL −1 , consuming as little 3 fluid. Double‐blind testing clinical DES samples using sensor commercial ELISA shows no significant difference between them. Compared single‐biomarker diagnosis, diagnostic accuracy this based biomarkers has improved by ≈16%. system offers potential enable personalized accurate diagnosis ocular diseases.

Язык: Английский

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Advances and applications in single-cell and spatial genomics

Science China Life Sciences, Год журнала: 2024, Номер unknown

Опубликована: Дек. 20, 2024

Язык: Английский

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