Nature Communications, Год журнала: 2024, Номер 15(1)
Опубликована: Сен. 5, 2024
Язык: Английский
Angewandte Chemie International Edition, Год журнала: 2024, Номер unknown
Опубликована: Июль 9, 2024
Amplifying DNA conjugated affinity ligands can improve the sensitivity and multiplicity of cell imaging play a crucial role in comprehensively deciphering cellular heterogeneity dynamic changes during development disease. However, one-step, controllable, quantitative amplification methods for multiplexed live-cell membrane proteins is challenging. Here, we introduce template adhesion reaction (TAR) method assembling amplifiable sequences with different ligands, such as aptamers or antibodies, amplified high fidelity. The precisely controllable TAR enables proportional protein targets variable abundances by modulating concentration ratios hairpin templates primers, thus allowing sensitive visualization multiple enhanced signal-to-noise (SNRs) without disturbing their original ratios. Using TAR, achieved signal-enhanced six on same within 1-2 h. represents an innovative programmable molecular toolkit profiling live-cells.
Язык: Английский
Процитировано
5
ACS Applied Materials & Interfaces, Год журнала: 2022, Номер 14(34), С. 39396 - 39403
Опубликована: Авг. 17, 2022
Various DNA assembly techniques and structures have emerged with the continuous progress of nanotechnology. hybridization chain reaction (HCR) is a representative example owing to isothermal enzyme-free features. However, HCR time consuming inhibited by nucleases present in biological samples. Herein, we demonstrated that cationic copolymer, poly(l-lysine)-graft-dextran (PLL-g-Dex), significantly facilitated increased its initiator sensitivity 40-fold. PLL-g-Dex promoted generation products high molecular weight accelerating initiation subsequent growth steps HCR. Moreover, protected system from nucleases, permitting presence serum components. Addition universal efficient strategy does not require optimization reactor setup or sequences, thus laying solid foundation for wider application
Язык: Английский
Процитировано
17
ACS Nano, Год журнала: 2023, Номер 18(1), С. 618 - 629
Опубликована: Дек. 28, 2023
The hybrid chain reaction (HCR), an isothermal and enzyme-free amplification strategy, has found extensive use in fluorescent
Язык: Английский
Процитировано
10
Journal of the American Chemical Society, Год журнала: 2024, Номер 146(24), С. 16428 - 16439
Опубликована: Июнь 11, 2024
Color encoding plays a crucial role in painting, digital photography, and spectral analysis. Achieving accurate, target-responsive color at the molecular level has potential to revolutionize scientific research technological innovation, but significant challenges persist. Here, we propose multibit DNA self-assembly system based on computer-aided design (CAD) technology, enabling target-responsive, amplified level, termed fluorescence (FLUCO). As model, establish quaternary FLUCO using four-bit self-assembly, which can accurately encode 51 colors, presenting immense applications such as spatial proteomic imaging multitarget Notably, enables simultaneous of multiple targets exceeding limitations channels conventional equipment, marks integration computer science for decoding. Overall, our work paves way controllable encoding, facilitating omics analysis, exfoliated cell high-throughput liquid biopsy.
Язык: Английский
Процитировано
4
Biosensors, Год журнала: 2024, Номер 14(6), С. 274 - 274
Опубликована: Май 27, 2024
A fluorogenic aptamer (FA)-based hybridization chain reaction (HCR) could provide a sensitive and label-free signal amplification method for imaging molecules in living cells. However, existing FA-HCR methods usually face some problems, such as complicated design significant background leakage, which greatly limit their application. Herein, we developed an FA-centered HCR (FAC-HCR) based on remote toehold-mediated strand displacement reaction. Compared to traditional HCRs mediated by four hairpin probes (HPs) two HPs, the FAC-HCR displayed significantly decreased leakage improved sensitivity. Furthermore, was used test non-nucleic acid target, apurinic/apyrimidinic endonuclease 1 (APE1), important BER-involved endonuclease. The fluorescence analysis results confirmed that can reach detection of 0.1174 U/mL. By using HPs with polyetherimide-based nanoparticles, activity APE1 cells be imaged. In summary, this study new idea FA-based improve performance live cell imaging.
Язык: Английский
Процитировано
3
ACS Biomaterials Science & Engineering, Год журнала: 2024, Номер 10(9), С. 5595 - 5608
Опубликована: Авг. 15, 2024
Analysis of biomarkers in living cells is crucial for deciphering the dynamics as well precise diagnosis diseases. DNA biosensors employ sequences probes to offer insights into cells, and drive progress disease drug development. In this review, we present recent advances detecting cells. The basic structural components signal output method are presented. strategies crossing cell membrane also described, including coincubation, nanocarriers, nanoelectroporation techniques. Based on biomarker categorization, detail applications small molecules, RNAs, proteins, integrated targets Furthermore, future development directions summarized encourage further research growing field.
Язык: Английский
Процитировано
3
Science Advances, Год журнала: 2024, Номер 10(35)
Опубликована: Авг. 30, 2024
The transport of ions through biological ion channels is regulated not only by their structural characteristics but also the composition phospholipid membrane, which serves as a carrier for nanochannels. Inspired modulation currents lipid membrane composition, exemplified activation K
Язык: Английский
Процитировано
3
Nanoscale Advances, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Tetrahedral DNA frameworks as restricted nanotriggers control HCR self-assembled living polymerization, thus achieving homogeneous product control.
Язык: Английский
Процитировано
0
Nature Communications, Год журнала: 2025, Номер 16(1)
Опубликована: Янв. 22, 2025
Understanding the heterogeneity of epigenetic modifications within single cells is pivotal for unraveling nature complexity gene expression and cellular function. In this study, we have developed a strategy based on multichrome encoding "AND" Boolean logic recognition multiplexed, spatially resolved imaging single-cell RNA modifications, termed as PRoximity Exchange-assisted Encoding Multichrome (PREEM). Through implementation strategy, can now map nuclear distribution multiple site-specific N6-methyladenosine (m6A) at single-molecule resolution level in single-cells, reveal previously unknown heterogeneity. Notably, demonstrate how these patterns change after treatment with various drugs. Moreover, cyclic tailed DNA self-assembly further suggest scalability adaptability PREEM's design. As an innovative modification tool, PREEM not only broadens horizons epigenetics research, enabling joint analysis targets beyond limitations channels, but also reveals cell-to-cell variability, thereby enhancing our capacity to explore functions. Here, authors recognition, PREEM, multiplexed m6A RNA.
Язык: Английский
Процитировано
0
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Фев. 14, 2025
mRNA, a critical biomarker for various diseases and promising target cancer therapy, is central to biological medical research. However, the development of multiplexed approaches in situ monitoring mRNA live cells are limited by their reliance on enzyme-based signal amplification, challenges with diffusion, complexity nucleic acid design. In this study, we introduce nonenzymatic catalytic DNA assembly (NEDA) technique address these limitations. NEDA facilitates precise imaging intracellular assembling three free hairpin amplifiers into low-mobility, three-dimensional spherical structure. This approach also enables simultaneous detection four distinct targets via combination fluorescent signals, limit as low 141.2 pM mRNA. To enhance efficiency design, employed computer-aided design (CAD) rapidly generate feasible sequences highly detection. By integrating machine learning algorithms, achieved impressive accuracy nearly 96.66% distinguishing multiple cell types 87.80% identifying same type under different drug stimulation conditions. Notably, our platform can identify stimuli similar mechanisms action, highlighting its potential development. 3D sensing strategy CAD not only enhances ability image simultaneously but provides novel efficient molecular diagnostics personalized therapy.
Язык: Английский
Процитировано
0