Cell Reports Physical Science,
Год журнала:
2023,
Номер
4(6), С. 101444 - 101444
Опубликована: Май 31, 2023
Endosomal
escape
remains
a
well-known
bottleneck
for
successful
small
interfering
RNA
(siRNA)
therapeutics.
Here,
chemical
electron
transfer
(CET)-based
self-activatable
lipopolyplex
is
explored
producing
singlet
oxygen
(1O2)
in
endosomes,
thereby
enhancing
siRNA
delivery.
The
simultaneously
encapsulates
bis(2,4,6-trichlorophenyl)
oxalate
(TCPO),
gold
nanoparticles
(GNs),
and
siRNA.
GNs
are
specifically
self-activated
by
the
transferred
energy
between
TCPO
hydrogen
peroxide,
which
enhanced
tumor
microenvironment.
Enhanced
endosomal
membrane
damage
verified
using
cytosolic
fluorescent
galectin-8
(Gal8)-mRuby
reporter;
oxidation
of
1O2
facilitates
rupture
endosomes
within
4
h,
improving
recruitment
Gal8
to
disrupted
up
∼50-fold.
This
research
demonstrates
that
CET
can
facilitate
nucleic
acids
from
cytosol
provides
starting
point
further
delivery
applications.
Pharmaceutics,
Год журнала:
2024,
Номер
16(11), С. 1362 - 1362
Опубликована: Окт. 25, 2024
Cationic
vaccines
of
nanometric
sizes
can
directly
perform
the
delivery
antigen(s)
and
immunomodulator(s)
to
dendritic
cells
in
lymph
nodes.
The
positively
charged
nanovaccines
are
taken
up
by
antigen-presenting
(APCs)
lymphatic
system
often
originating
cellular
immunological
defense
required
fight
intracellular
microbial
infections
proliferation
cancers.
molecules
imparting
positive
charges
exhibit
a
dose-dependent
toxicity
which
needs
be
systematically
addressed.
Against
coronavirus,
mRNA
cationic
evolved
rapidly.
Nowadays
have
been
formulated
against
several
with
advantage
compounds
granting
protection
nucleic
acids
vivo
biodegradation
nucleases.
Up
threshold
concentration
for
nanovaccine
delivery,
well
eliciting
desired
Th
1
improved
immune
response
absence
cytotoxicity.
A
second
strategy
literature
involves
dilution
components
biocompatible
polymeric
matrixes.
Polymeric
nanoparticles
incorporating
at
reduced
concentrations
component
result
an
toxic
effects.
progress
vaccinology
cancer
situ
designs
nanovaccines.
lysis
transformed
releases
tumoral
antigens,
presence
nanoadjuvants
systemically
presented
prevention
metastatic
cancer.
In
addition,
these
local
allow
immunotherapeutic
tumor
treatment.
Exploration of Medicine,
Год журнала:
2023,
Номер
unknown, С. 782 - 812
Опубликована: Окт. 31, 2023
The
prevalence
of
skin
cancer
has
increased
hastily
in
the
recent
decade
for
both
kinds
melanoma
and
non-melanoma
cancer.
Skin
cancers
mostly
encompass
keratinocyte
cancers:
cutaneous
squamous
cell
carcinoma,
basal
melanoma.
This
review
discusses
advancements
treatment
In
addition
to
chemotherapy,
immunotherapy,
targeted
therapy,
photodynamic
therapy
(PDT),
there
are
several
other
therapies
Additionally,
PDT
use
combination
with
radiation,
surgery
is
being
actively
investigated.
will
specifically
address
pathophysiology
cancer,
diagnostic
approaches,
current
used
topical
introduce
emerging
using
nanotechnology
that
may
be
beneficial
these
indications.
Cell Reports Physical Science,
Год журнала:
2023,
Номер
4(6), С. 101444 - 101444
Опубликована: Май 31, 2023
Endosomal
escape
remains
a
well-known
bottleneck
for
successful
small
interfering
RNA
(siRNA)
therapeutics.
Here,
chemical
electron
transfer
(CET)-based
self-activatable
lipopolyplex
is
explored
producing
singlet
oxygen
(1O2)
in
endosomes,
thereby
enhancing
siRNA
delivery.
The
simultaneously
encapsulates
bis(2,4,6-trichlorophenyl)
oxalate
(TCPO),
gold
nanoparticles
(GNs),
and
siRNA.
GNs
are
specifically
self-activated
by
the
transferred
energy
between
TCPO
hydrogen
peroxide,
which
enhanced
tumor
microenvironment.
Enhanced
endosomal
membrane
damage
verified
using
cytosolic
fluorescent
galectin-8
(Gal8)-mRuby
reporter;
oxidation
of
1O2
facilitates
rupture
endosomes
within
4
h,
improving
recruitment
Gal8
to
disrupted
up
∼50-fold.
This
research
demonstrates
that
CET
can
facilitate
nucleic
acids
from
cytosol
provides
starting
point
further
delivery
applications.
