Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Июнь 6, 2023
Abstract
Acoustic
tweezers
can
control
target
movement
through
the
momentum
interaction
between
an
acoustic
wave
and
object.
This
technology
has
advantages
over
optical
for
in-vivo
cell
manipulation
due
to
its
high
tissue
penetrability
strong
radiation
force.
However,
normal
cells
are
difficult
acoustically
manipulate
because
of
their
small
size
similarity
impedance
that
medium.
In
this
study,
we
use
heterologous
expression
gene
clusters
generate
genetically
engineered
bacteria
produce
numerous
sub-micron
gas
vesicles
in
bacterial
cytoplasm.
We
show
presence
significantly
enhances
sensitivity
engineering
bacteria,
which
be
manipulated
by
ultrasound.
find
employing
phased-array-based
tweezers,
trapped
into
vitro
vivo
via
electronically
steered
beams,
enabling
counter
flow
or
on-demand
these
vasculature
live
mice.
Furthermore,
demonstrate
aggregation
efficiency
a
tumour
is
improved
utilizing
technology.
study
provides
platform
cells,
will
promote
progress
cell-based
biomedical
applications.
Structure,
Год журнала:
2023,
Номер
31(5), С. 518 - 528.e6
Опубликована: Апрель 10, 2023
Gas
vesicles
(GVs)
are
gas-filled
protein
nanostructures
employed
by
several
species
of
bacteria
and
archaea
as
flotation
devices
to
enable
access
optimal
light
nutrients.
The
unique
physical
properties
GVs
have
led
their
use
genetically
encodable
contrast
agents
for
ultrasound
MRI.
Currently,
however,
the
structure
assembly
mechanism
remain
unknown.
Here
we
employ
cryoelectron
tomography
reveal
how
GV
shell
is
formed
a
helical
filament
highly
conserved
GvpA
subunits.
This
changes
polarity
at
center
cylinder,
site
that
may
act
an
elongation
center.
Subtomogram
averaging
reveals
corrugated
pattern
arising
from
polymerization
into
β
sheet.
accessory
GvpC
forms
cage
around
shell,
providing
structural
reinforcement.
Together,
our
results
help
explain
remarkable
mechanical
ability
adopt
different
diameters
shapes.
Angewandte Chemie International Edition,
Год журнала:
2024,
Номер
63(13)
Опубликована: Янв. 10, 2024
Abstract
Ultrasound
technology,
synergistically
harnessed
with
genetic
engineering
and
chemistry
concepts,
has
started
to
open
the
gateway
remarkable
realm
of
sonogenetics—a
pioneering
paradigm
for
remotely
orchestrating
cellular
functions
at
molecular
level.
This
fusion
not
only
enables
precisely
targeted
imaging
therapeutic
interventions,
but
also
advances
our
comprehension
mechanobiology
unparalleled
depths.
Sonogenetic
tools
harness
mechanical
force
within
small
tissue
volumes
while
preserving
integrity
surrounding
physiological
environment,
reaching
depths
up
tens
centimeters
high
spatiotemporal
precision.
These
capabilities
circumvent
inherent
physical
limitations
alternative
in
vivo
control
methods
such
as
optogenetics
magnetogenetics.
In
this
review,
we
first
discuss
mechanosensitive
ion
channels,
most
commonly
utilized
sonogenetic
mediators,
both
mammalian
non‐mammalian
systems.
Subsequently,
provide
a
comprehensive
overview
state‐of‐the‐art
approaches
that
leverage
thermal
or
features
ultrasonic
waves.
Additionally,
explore
strategies
centered
around
design
mechanochemically
reactive
macromolecular
Furthermore,
delve
into
ultrasound
biomolecular
function,
encompassing
utilization
gas
vesicles
acoustic
reporter
genes.
Finally,
shed
light
on
challenges
sonogenetics
present
perspective
future
promising
technology.
Advanced Materials,
Год журнала:
2023,
Номер
35(33)
Опубликована: Май 24, 2023
Abstract
Ultrasound
(US)‐triggered
cascade
amplification
of
nanotherapies
has
attracted
considerable
attention
as
an
effective
strategy
for
cancer
treatment.
With
the
remarkable
advances
in
materials
chemistry
and
nanotechnology,
a
large
number
well‐designed
nanosystems
have
emerged
that
incorporate
presupposed
processes
can
be
activated
to
trigger
therapies
such
chemotherapy,
immunotherapy,
ferroptosis,
under
exogenous
US
stimulation
or
specific
substances
generated
by
actuation,
maximize
antitumor
efficacy
minimize
detrimental
effects.
Therefore,
summarizing
corresponding
applications
based
on
US‐triggered
is
essential.
This
review
comprehensively
summarizes
highlights
recent
design
intelligent
modalities,
consisting
unique
components,
distinctive
properties,
processes.
These
ingenious
strategies
confer
unparalleled
potential
ultrasound‐triggered
provide
superior
controllability,
thus
overcoming
unmet
requirements
precision
medicine
personalized
Finally,
challenges
prospects
this
emerging
are
discussed
it
expected
encourage
more
innovative
ideas
promote
their
further
development.
Advanced Materials,
Год журнала:
2024,
Номер
36(28)
Опубликована: Март 27, 2024
Ultrasound
imaging
and
ultrasound-mediated
gene
drug
delivery
are
rapidly
advancing
diagnostic
therapeutic
methods;
however,
their
use
is
often
limited
by
the
need
for
microbubbles,
which
cannot
transverse
many
biological
barriers
due
to
large
size.
Here,
authors
introduce
50-nm
gas-filled
protein
nanostructures
derived
from
genetically
engineered
gas
vesicles(GVs)
that
referred
as
