Frontiers in Cell and Developmental Biology,
Год журнала:
2023,
Номер
11
Опубликована: Фев. 16, 2023
The
corticotropin-releasing
hormone
(CRH)-expressing
neurons
in
the
hypothalamus
are
critical
regulators
of
neuroendocrine
stress
response
pathway,
known
as
hypothalamic-pituitary-adrenal
(HPA)
axis.
As
developmental
vulnerabilities
CRH
contribute
to
stress-associated
neurological
and
behavioral
dysfunctions,
it
is
identify
mechanisms
underlying
normal
abnormal
neuron
development.
Using
zebrafish,
we
identified
Down
syndrome
cell
adhesion
molecule
like-1
(
dscaml1
)
an
integral
mediator
development
necessary
for
establishing
axis
function.
In
mutant
animals,
hypothalamic
had
higher
crhb
(the
homolog
zebrafish)
expression,
increased
number,
reduced
death
compared
wild-type
controls.
Physiologically,
animals
baseline
(cortisol)
levels
attenuated
responses
acute
stressors.
Together,
these
findings
essential
factor
suggest
that
HPA
dysregulation
may
etiology
human
DSCAML1
-linked
neuropsychiatric
disorders.
Nature Methods,
Год журнала:
2022,
Номер
19(11), С. 1357 - 1366
Опубликована: Окт. 24, 2022
Abstract
Dense
reconstruction
of
synaptic
connectivity
requires
high-resolution
electron
microscopy
images
entire
brains
and
tools
to
efficiently
trace
neuronal
wires
across
the
volume.
To
generate
such
a
resource,
we
sectioned
imaged
larval
zebrafish
brain
by
serial
block-face
at
voxel
size
14
×
25
nm
3
.
We
segmented
resulting
dataset
with
flood-filling
network
algorithm,
automated
detection
chemical
synapses
validated
results
comparisons
transmission
microscopic
light-microscopic
reconstructions.
Neurons
their
connections
are
stored
in
form
queryable
expandable
digital
address
book.
reconstructed
208
neurons
involved
visual
motion
processing,
most
them
located
pretectum,
which
had
been
functionally
characterized
same
specimen
two-photon
calcium
imaging.
Moreover,
mapped
all
407
presynaptic
postsynaptic
partners
two
superficial
interneurons
tectum.
The
resource
developed
here
serves
as
foundation
for
synaptic-resolution
circuit
analyses
nervous
system.
Abstract
Neuronal
phenotypic
traits
such
as
morphology,
connectivity
and
function
are
dictated,
to
a
large
extent,
by
specific
combination
of
differentially
expressed
genes.
Clusters
neurons
in
transcriptomic
space
correspond
distinct
cell
types
some
cases—for
example,
Caenorhabditis
elegans
1
retinal
ganglion
cells
2–4
—have
been
shown
share
morphology
function.
The
zebrafish
optic
tectum
is
composed
spatial
array
that
transforms
visual
inputs
into
motor
outputs.
Although
the
visuotopic
map
continuous,
subregions
functionally
specialized
5,6
.
Here,
uncover
cell-type
architecture
tectum,
we
transcriptionally
profiled
its
neurons,
revealing
more
than
60
organized
anatomical
layers.
We
measured
responses
thousands
tectal
two-photon
calcium
imaging
matched
them
with
their
transcriptional
profiles.
Furthermore,
characterized
morphologies
identified
using
transgenic
lines.
Notably,
found
similar
can
diverge
shape,
responses.
Incorporating
coordinates
within
volume
revealed
morphologically
defined
subclusters
individual
clusters.
Our
findings
demonstrate
extrinsic,
position-dependent
factors
expand
repertoire
genetically
neurons.
Nature,
Год журнала:
2022,
Номер
608(7921), С. 146 - 152
Опубликована: Июль 13, 2022
Abstract
Social
affiliation
emerges
from
individual-level
behavioural
rules
that
are
driven
by
conspecific
signals
1–5
.
Long-distance
attraction
and
short-distance
repulsion,
for
example,
jointly
set
a
preferred
interanimal
distance
in
swarms
6–8
However,
little
is
known
about
their
perceptual
mechanisms
executive
neural
circuits
3
Here
we
trace
the
neuronal
response
to
self-like
biological
motion
9,10
,
visual
trigger
developing
zebrafish
2,11
Unbiased
activity
mapping
targeted
volumetric
two-photon
calcium
imaging
revealed
21
hotspots
distributed
throughout
brain
as
well
clustered
biological-motion-tuned
neurons
multimodal,
socially
activated
nucleus
of
dorsal
thalamus.
Individual
thalamus
encode
local
acceleration
stimuli
mimicking
typical
fish
kinetics
but
insensitive
global
or
continuous
motion.
Electron
microscopic
reconstruction
synaptic
input
optic
tectum
projections
into
hypothalamic
areas
with
conserved
social
function
12–14
Ablation
selectively
disrupted
without
affecting
repulsion.
This
tectothalamic
pathway
thus
serves
recognition
conspecifics,
dissociates
control
repulsion
during
affiliation,
revealing
circuit
underpinning
collective
behaviour.
Annual Review of Neuroscience,
Год журнала:
2024,
Номер
47(1), С. 255 - 276
Опубликована: Апрель 25, 2024
The
zebrafish
visual
system
has
become
a
paradigmatic
preparation
for
behavioral
and
systems
neuroscience.
Around
40
types
of
retinal
ganglion
cells
(RGCs)
serve
as
matched
filters
stimulus
features,
including
light,
optic
flow,
prey,
objects
on
collision
course.
RGCs
distribute
their
signals
via
axon
collaterals
to
12
retinorecipient
areas
in
forebrain
midbrain.
major
visuomotor
hub,
the
tectum,
harbors
nine
RGC
input
layers
that
combine
information
multiple
features.
retinotopic
map
tectum
is
locally
adapted
scene
statistics
subfield–specific
demands.
Tectal
projections
premotor
centers
are
topographically
organized
according
commands.
known
connectivity
more
than
20
processing
streams
allows
us
dissect
cellular
basis
elementary
perceptual
cognitive
functions.
Visually
evoked
responses,
such
prey
capture
or
loom
avoidance,
controlled
by
dedicated
multistation
pathways
that—at
least
larva—resemble
labeled
lines.
This
architecture
serves
neuronal
code's
purpose
driving
adaptive
behavior.
Cellular and Molecular Life Sciences,
Год журнала:
2023,
Номер
80(8)
Опубликована: Июль 25, 2023
Abstract
The
cerebellum
represents
a
brain
compartment
that
first
appeared
in
gnathostomes
(jawed
vertebrates).
Besides
the
addition
of
cell
numbers,
its
development,
cytoarchitecture,
circuitry,
physiology,
and
function
have
been
highly
conserved
throughout
avian
mammalian
species.
While
cerebellar
research
mammals
is
extensive,
systematic
investigations
on
this
zebrafish
as
teleostian
model
organism
started
only
about
two
decades
ago,
but
has
provided
considerable
insight
into
since
then.
Zebrafish
are
genetically
tractable
with
nearly
transparent
small-sized
embryos,
which
development
occurs
within
few
days.
Therefore,
genetic
accompanied
non-invasive
high-resolution
vivo
time-lapse
imaging
powerful
combination
for
interrogating
behavior
cells
their
complex
native
environment.
Molecular Psychiatry,
Год журнала:
2024,
Номер
29(4), С. 1046 - 1062
Опубликована: Янв. 17, 2024
Abstract
Serotonergic
psychedelics
are
emerging
therapeutics
for
psychiatric
disorders,
yet
their
underlying
mechanisms
of
action
in
the
brain
remain
largely
elusive.
Here,
we
developed
a
wide-field
behavioral
tracking
system
larval
zebrafish
and
investigated
effects
psilocybin,
psychedelic
serotonin
receptor
agonist.
Machine
learning
analyses
precise
body
kinematics
identified
latent
states
reflecting
spontaneous
exploration,
visually-driven
rapid
swimming,
irregular
swim
patterns
following
stress
exposure.
Using
this
method,
found
that
acute
psilocybin
treatment
has
two
effects:
[i]
facilitation
exploration
(“stimulatory”)
[ii]
prevention
exposure
(“anxiolytic”).
These
differed
from
effect
SSRI
were
rather
similar
to
ketamine
treatment.
Neural
activity
imaging
dorsal
raphe
nucleus
suggested
inhibits
serotonergic
neurons
by
activating
local
GABAergic
neurons,
consistent
with
psychedelic-induced
suppression
mammals.
findings
pave
way
using
elucidate
neural
psychedelics.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Май 23, 2024
Both
neurons
and
glia
communicate
via
diffusible
neuromodulatory
substances,
but
the
substrates
of
computation
in
such
networks
are
unclear.
During
behavioral
transitions
larval
zebrafish,
neuromodulator
norepinephrine
drives
fast
excitation
delayed
inhibition
behavior
circuit
activity.
We
find
that
inhibitory
arm
this
feedforward
motif
is
implemented
by
astroglial
purinergic
signaling.
Neuromodulator
imaging,
pharmacology,
perturbations
astroglia
reveal
triggers
release
adenosine
triphosphate,
extracellular
conversion
into
adenosine,
suppression
through
activation
hindbrain
neuronal
receptors.
This
work,
along
with
a
companion
piece
Lefton
colleagues
demonstrating
an
analogous
pathway
mediating
effect
on
synaptic
connectivity
mice,
identifies
computational
role
for
evolutionarily
conserved
signaling
axis
norepinephrine-mediated
brain
state
transitions.
