Zebrafish brain atlases: a collective effort for a tiny vertebrate brain DOI Creative Commons

Antoine Légaré,

Mado Lemieux, Patrick Desrosiers

et al.

Neurophotonics, Journal Year: 2023, Volume and Issue: 10(04)

Published: Sept. 30, 2023

In the past two decades, digital brain atlases have emerged as essential tools for sharing and integrating complex neuroscience datasets. Concurrently, larval zebrafish has become a prominent vertebrate model offering strategic compromise size, complexity, transparency, optogenetic access, behavior. We provide brief overview of recently developed brain, intersecting neuroanatomical information, gene expression patterns, connectivity. These are becoming pivotal by centralizing large datasets while supporting generation circuit hypotheses functional measurements can be registered into an atlas' standard coordinate system to interrogate its structural database. As challenges persist in mapping neural circuits incorporating atlases, we emphasize importance collaborative efforts standardized protocols expand these resources crack codes neuronal activity guiding behavior this tiny brain.

Language: Английский

Automated synapse-level reconstruction of neural circuits in the larval zebrafish brain DOI Creative Commons

Fabian Svara,

Dominique Förster, Fumi Kubo

et al.

Nature Methods, Journal Year: 2022, Volume and Issue: 19(11), P. 1357 - 1366

Published: Oct. 24, 2022

Abstract Dense reconstruction of synaptic connectivity requires high-resolution electron microscopy images entire brains and tools to efficiently trace neuronal wires across the volume. To generate such a resource, we sectioned imaged larval zebrafish brain by serial block-face at voxel size 14 × 25 nm 3 . We segmented resulting dataset with flood-filling network algorithm, automated detection chemical synapses validated results comparisons transmission microscopic light-microscopic reconstructions. Neurons their connections are stored in form queryable expandable digital address book. reconstructed 208 neurons involved visual motion processing, most them located pretectum, which had been functionally characterized same specimen two-photon calcium imaging. Moreover, mapped all 407 presynaptic postsynaptic partners two superficial interneurons tectum. The resource developed here serves as foundation for synaptic-resolution circuit analyses nervous system.

Language: Английский

Citations

76
Transcriptomic neuron types vary topographically in function and morphology DOI Creative Commons
Inbal Shainer, Johannes M. Kappel,

Eva Laurell

et al.

Nature, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Abstract Neuronal phenotypic traits such as morphology, connectivity and function are dictated, to a large extent, by specific combination of differentially expressed genes. Clusters neurons in transcriptomic space correspond distinct cell types some cases—for example, Caenorhabditis elegans 1 retinal ganglion cells 2–4 —have been shown share morphology function. The zebrafish optic tectum is composed spatial array that transforms visual inputs into motor outputs. Although the visuotopic map continuous, subregions functionally specialized 5,6 . Here, uncover cell-type architecture tectum, we transcriptionally profiled its neurons, revealing more than 60 organized anatomical layers. We measured responses thousands tectal two-photon calcium imaging matched them with their transcriptional profiles. Furthermore, characterized morphologies identified using transgenic lines. Notably, found similar can diverge shape, responses. Incorporating coordinates within volume revealed morphologically defined subclusters individual clusters. Our findings demonstrate extrinsic, position-dependent factors expand repertoire genetically neurons.

Language: Английский

Citations

4
Visual recognition of social signals by a tectothalamic neural circuit DOI Creative Commons
Johannes M. Kappel, Dominique Förster, Katja Slangewal

et al.

Nature, Journal Year: 2022, Volume and Issue: 608(7921), P. 146 - 152

Published: July 13, 2022

Abstract Social affiliation emerges from individual-level behavioural rules that are driven by conspecific signals 1–5 . Long-distance attraction and short-distance repulsion, for example, jointly set a preferred interanimal distance in swarms 6–8 However, little is known about their perceptual mechanisms executive neural circuits 3 Here we trace the neuronal response to self-like biological motion 9,10 , visual trigger developing zebrafish 2,11 Unbiased activity mapping targeted volumetric two-photon calcium imaging revealed 21 hotspots distributed throughout brain as well clustered biological-motion-tuned neurons multimodal, socially activated nucleus of dorsal thalamus. Individual thalamus encode local acceleration stimuli mimicking typical fish kinetics but insensitive global or continuous motion. Electron microscopic reconstruction synaptic input optic tectum projections into hypothalamic areas with conserved social function 12–14 Ablation selectively disrupted without affecting repulsion. This tectothalamic pathway thus serves recognition conspecifics, dissociates control repulsion during affiliation, revealing circuit underpinning collective behaviour.

Language: Английский

Citations

60
The Visual Systems of Zebrafish DOI
Herwig Baier, Ethan K. Scott

Annual Review of Neuroscience, Journal Year: 2024, Volume and Issue: 47(1), P. 255 - 276

Published: April 25, 2024

The zebrafish visual system has become a paradigmatic preparation for behavioral and systems neuroscience. Around 40 types of retinal ganglion cells (RGCs) serve as matched filters stimulus features, including light, optic flow, prey, objects on collision course. RGCs distribute their signals via axon collaterals to 12 retinorecipient areas in forebrain midbrain. major visuomotor hub, the tectum, harbors nine RGC input layers that combine information multiple features. retinotopic map tectum is locally adapted scene statistics subfield–specific demands. Tectal projections premotor centers are topographically organized according commands. known connectivity more than 20 processing streams allows us dissect cellular basis elementary perceptual cognitive functions. Visually evoked responses, such prey capture or loom avoidance, controlled by dedicated multistation pathways that—at least larva—resemble labeled lines. This architecture serves neuronal code's purpose driving adaptive behavior.

Language: Английский

Citations

9
Development, circuitry, and function of the zebrafish cerebellum DOI Creative Commons
Sol Pose‐Méndez, Paul J. Schramm,

Komali Valishetti

et al.

Cellular and Molecular Life Sciences, Journal Year: 2023, Volume and Issue: 80(8)

Published: July 25, 2023

Abstract The cerebellum represents a brain compartment that first appeared in gnathostomes (jawed vertebrates). Besides the addition of cell numbers, its development, cytoarchitecture, circuitry, physiology, and function have been highly conserved throughout avian mammalian species. While cerebellar research mammals is extensive, systematic investigations on this zebrafish as teleostian model organism started only about two decades ago, but has provided considerable insight into since then. Zebrafish are genetically tractable with nearly transparent small-sized embryos, which development occurs within few days. Therefore, genetic accompanied non-invasive high-resolution vivo time-lapse imaging powerful combination for interrogating behavior cells their complex native environment.

Language: Английский

Citations

19
High-resolution tracking of unconfined zebrafish behavior reveals stimulatory and anxiolytic effects of psilocybin DOI Creative Commons

Dotan Braun,

Ayelet M Rosenberg,

Elad Rabaniam

et al.

Molecular Psychiatry, Journal Year: 2024, Volume and Issue: 29(4), P. 1046 - 1062

Published: Jan. 17, 2024

Abstract Serotonergic psychedelics are emerging therapeutics for psychiatric disorders, yet their underlying mechanisms of action in the brain remain largely elusive. Here, we developed a wide-field behavioral tracking system larval zebrafish and investigated effects psilocybin, psychedelic serotonin receptor agonist. Machine learning analyses precise body kinematics identified latent states reflecting spontaneous exploration, visually-driven rapid swimming, irregular swim patterns following stress exposure. Using this method, found that acute psilocybin treatment has two effects: [i] facilitation exploration (“stimulatory”) [ii] prevention exposure (“anxiolytic”). These differed from effect SSRI were rather similar to ketamine treatment. Neural activity imaging dorsal raphe nucleus suggested inhibits serotonergic neurons by activating local GABAergic neurons, consistent with psychedelic-induced suppression mammals. findings pave way using elucidate neural psychedelics.

Language: Английский

Citations

7
Norepinephrine changes behavioral state via astroglial purinergic signaling DOI Creative Commons
Alex Chen, Marc Duque, Vickie M. Wang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 23, 2024

Both neurons and glia communicate via diffusible neuromodulatory substances, but the substrates of computation in such networks are unclear. During behavioral transitions larval zebrafish, neuromodulator norepinephrine drives fast excitation delayed inhibition behavior circuit activity. We find that inhibitory arm this feedforward motif is implemented by astroglial purinergic signaling. Neuromodulator imaging, pharmacology, perturbations astroglia reveal triggers release adenosine triphosphate, extracellular conversion into adenosine, suppression through activation hindbrain neuronal receptors. This work, along with a companion piece Lefton colleagues demonstrating an analogous pathway mediating effect on synaptic connectivity mice, identifies computational role for evolutionarily conserved signaling axis norepinephrine-mediated brain state transitions.

Language: Английский

Citations

7
Designing a Hybrid Chain Reaction Probe for Multiplex Transcripts Assay with High-Level Imaging DOI
Dongjian Cao,

Xinxin Qin,

Wenjing Wang

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 18(1), P. 618 - 629

Published: Dec. 28, 2023

The hybrid chain reaction (HCR), an isothermal and enzyme-free amplification strategy, has found extensive use in fluorescent

Language: Английский

Citations

10
Using Zebrafish to Study the Mechanisms That Underlie Down Syndrome DOI
Anna J. Moyer, Summer B. Thyme

Published: Jan. 1, 2025

Language: Английский

Citations

0
Spatial mRNA Profiling Using Rapid Amplified Multiplexed-FISH (RAM-FISH) DOI
Tirtha Das Banerjee, J. Dale Raine, Ajay S. Mathuru

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0