Physiological functions of RIG-I-like receptors

Immunity, Год журнала: 2024, Номер 57(4), С. 731 - 751

Опубликована: Апрель 1, 2024

Язык: Английский

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Plant and animal positive-sense single-stranded RNA viruses encode small proteins important for viral infection in their negative-sense strand

Molecular Plant, Год журнала: 2023, Номер 16(11), С. 1794 - 1810

Опубликована: Сен. 29, 2023

Язык: Английский

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Current Perspectives on Functional Involvement of Micropeptides in Virus–Host Interactions

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3651 - 3651

Опубликована: Апрель 12, 2025

Micropeptides (miPEPs), encoded by short open reading frames (sORFs) within various genomic regions, have recently emerged as critical regulators of multiple biological processes. In particular, these small molecules are now increasingly being recognized for their role in modulating viral replication, pathogenesis, and host immune responses. Both miPEPs virus-derived been noted ability to regulate virus–host interactions through diversified mechanisms such altering protein stability protein–protein interactions. Although thousands sORFs annotated having the potential encode miPEPs, only a number experimentally validated so far, with some directly linked subset associated modulation, indicating that investigation is still its infancy. The systematic identification, translational status assessment, in-depth characterization, functional analysis substantial fraction encoding remain largely underexplored. Further studies anticipated uncover intricate underlying interactions, broader functions miPEPs. This article will review emerging roles immunity, discuss challenges future perspectives miPEP studies.

Язык: Английский

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Emerging roles for microproteins as critical regulators of endoplasmic reticulum function and cellular homeostasis

Seminars in Cell and Developmental Biology, Год журнала: 2025, Номер 170, С. 103608 - 103608

Опубликована: Апрель 17, 2025

Язык: Английский

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Microproteins in cancer: identification, biological functions, and clinical implications

Trends in Genetics, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

HighlightsMicroproteins, historically overlooked, are emerging as key players in cancer biology.Advances genomics and proteomics enable systematic identification of cancer-associated microproteins.Microproteins contribute to pathogenesis, an important source cryptic antigens, may offer novel therapeutic targets.Harnessing the potential microproteins could revolutionize diagnostics, therapeutics, immunotherapy.AbstractCancer continues be a major global health challenge, accounting for 10 million deaths annually worldwide. Since inception genome-wide sequencing studies 20 years ago, core set ~700 oncogenes tumor suppressor genes has become basis research. However, this research been based largely on understanding that human genome encodes ~19 500 protein-coding genes. Complementing genomic landscape, recent advances have described numerous which now poised redefine our oncogenic processes open new avenues intervention. This review explores evidence microprotein involvement mechanisms discusses applications, with emphasis highlighting field.

Язык: Английский

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Deeper look into viruses: replication intermediates do code!

Plant Cell Reports, Год журнала: 2024, Номер 43(2)

Опубликована: Фев. 1, 2024

Язык: Английский

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Noncanonical microprotein regulation of immunity

Molecular Therapy, Год журнала: 2024, Номер 32(9), С. 2905 - 2929

Опубликована: Май 11, 2024

Язык: Английский

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Small but mighty: the rise of microprotein biology in neuroscience

Frontiers in Molecular Neuroscience, Год журнала: 2024, Номер 17

Опубликована: Май 14, 2024

The mammalian central nervous system coordinates a network of signaling pathways and cellular interactions, which enable myriad complex cognitive physiological functions. While traditional efforts to understand the molecular basis brain function have focused on well-characterized proteins, recent advances in high-throughput translatome profiling revealed staggering number proteins translated from non-canonical open reading frames (ncORFs) such as 5' 3' untranslated regions annotated out-of-frame internal ORFs, previously non-coding RNAs. Of note, microproteins < 100 amino acids (AA) that are ncORFs often been neglected due computational biochemical challenges. Thousands putative identified cell lines tissues including brain, with some serving critical biological In this perspective, we highlight discovery describe several hypotheses emerged concerning microprotein developing mature system.

Язык: Английский

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Targeting PRMT7-mediated monomethylation of MAVS enhances antiviral innate immune responses and inhibits RNA virus replication

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(47)

Опубликована: Ноя. 15, 2024

RIG-I-like receptors (RLRs)-mitochondrial antiviral signaling protein (MAVS) are crucial for type I interferon (IFN) pathway and innate immune responses triggered by RNA viruses. However, the regulatory molecular mechanisms underlying virus-activated IFN remain incompletely understood. Here, we found that arginine methyltransferase 7 (PRMT7) serves as a negative regulator of interacting with MAVS catalyzing monomethylation 232 (R232me1) in MAVS. virus infection leads to downregulation dissociation PRMT7 from well decrease R232me1 methylation, enhancing MAVS/RIG-I interaction, aggregation, activation, responses. Knock-in mice R232 substituted lysine ( Mavs R232K -KI) more resistant Vesicular Stomatitis Virus due enhanced PiPRMT7-MAVS, short peptide inhibitor designed interrupt interaction between MAVS, inhibits thereby promoting activating signaling, bolstering suppress replication. Moreover, clinical relevance is highlighted it significantly downregulated virus-infected samples, such blood samples severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Ebola virus, H1N1-infected bronchial epithelial cells. Our findings uncovered PRMT7-mediated methylation plays critical roles regulating MAVS-mediated responses, targeting might represent therapeutic avenue treating viral infection.

Язык: Английский

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Application of Twin‐Strep Tagging and TurboID Proximity Labeling in Discovery of Microprotein‐protein Interactions

ChemistrySelect, Год журнала: 2024, Номер 9(23)

Опубликована: Июнь 18, 2024

Abstract Microproteins are a novel class of bioactive polypeptides that encoded by small open reading frames (smORFs). Emerging evidence has demonstrated these microproteins possess significant roles and undertake essential regulatory functions in living organisms. Meanwhile, several exhibit variable expression distinct regulation disease healthy cells, revealing potential therapeutic interventions. A promising strategy to characterize investigate their pathological association is through identification microprotein‐protein interactions (MPIs), emphasizing the value chemical biology tools for mapping MPIs. In this study, we applied twin‐strep tagging TurboID proximity labeling strategies unveil complicated cellular networks microproteins. Encouraged success both approaches identifying well‐studied CYREN‐Ku70/Ku80 complex, investigated an uncharacterized FAM229B microprotein found it interacts with thyroid receptor‐interacting protein 6 (TRIP6) may regulate its nuclear translocation. Our study exhibited encouraging performance bona fide interacting partners.

Язык: Английский

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