Programmable Split DNAzyme Modulators via Allosteric Cooperative Activation for mRNA Electrochemiluminescence Biosensing DOI

Liu-Qing Tan,

Weijia Zeng,

Qiaolin Chen

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

DNAzymes, known for their programmability, stability, and cost-effectiveness, are powerful tools signal transduction in complex biological systems. However, application responding to target effectors is often hindered by limited catalytic efficiency susceptibility unintended activation. Here we propose an allosteric cooperative activation strategy program a split DNAzyme modulator (STATER) that enables sensitive accurate electrochemiluminescence (ECL) biosensing of interleukin-6 (IL-6) mRNA. Our design features STATER leverages DNA tetrahedron as central scaffold, equipped with two pairs T-shaped hairpin probes (TP) helper (HP). Specifically, the TP contains apurinic/apyrimidinic endonuclease 1 (APE1) recognition sites, IL-6 mRNA region, partzyme fragment, while HP corresponding paired fragment. Unlike conventional modulators rely on single effector activation, integrates mechanism, which ensures all preblocked components synergistically activated assembled within confined space, facilitating rapid specific reconstruction DNAzyme's active domain. Furthermore, upon APE1 mRNA, inactive partzymes undergo assembly via toehold exchange displacement reaction, switching cleavage reactivity STATER. This mechanism establishment threshold thereby minimizing nonspecific scenarios. studies demonstrate exhibits outstanding sensitivity selectivity detection using supramolecular gold nanoclusters network-based ECL platform. The biosensor provides linear span from × 10–13 10–7 M, limit low 3.26 10–14 highlighting STATER's potential detecting various analytes

Язык: Английский

Integrated Microfluidic Chip for Neutrophil Extracellular Vesicle Analysis and Gastric Cancer Diagnosis DOI Creative Commons
Dan Yu, Jianmei Gu, Jiahui Zhang

и другие.

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Март 9, 2025

Neutrophil-derived extracellular vesicles (NEVs) are critically involved in disease progression and considered potential biomarkers. However, the tedious processes of NEV separation detection restrain their use. Herein, we presented an integrated microfluidic chip for (IMCN) analysis, which achieved immune-separation CD66b+ NEVs multiplexed contained miRNAs (termed signatures) by using 10 μL serum samples. The optimized microchannel flow rate IMCN enabled efficient capture (>90%). After recognition captured a specific CD63 aptamer, on-chip rolling circle amplification (RCA) reaction was triggered released aptamers from heat-lysed NEVs. Then, RCA products bound to molecular beacons (MBs), initiating allosteric hairpin structures amplified "turn on" fluorescence signals (RCA-MB assay). Clinical sample analysis showed that signatures had high area under curve (AUC) distinguishing between healthy control (HC) gastric cancer (GC) (0.891), benign diseases (BGD) GC (0.857). Notably, AUC reached 0.912 with combination five biomarkers (NEV signatures, CEA, CA199) differentiate HC, diagnostic accuracy further increased machine learning (ML)-based ensemble classification system. Therefore, developed is valuable platform may have use diagnosis.

Язык: Английский

Процитировано

1
Biosensor-based methods for exosome detection with applications to disease diagnosis DOI

Weikang Ge,

Zheying Mu,

Shiao Yang

и другие.

Biosensors and Bioelectronics, Год журнала: 2025, Номер unknown, С. 117362 - 117362

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

1
Dynamically controllable hot spots in DNA-derived hydrogel scaffold SERS substrate for exosome recognition using DNA self-assembly amplification DOI
Yeru Wang, Rongke Gao, Chao Ma

и другие.

Chemical Engineering Journal, Год журнала: 2024, Номер 496, С. 154270 - 154270

Опубликована: Июль 24, 2024

Язык: Английский

Процитировано

9
Hypoxia-Driven Changes in Tumor Microenvironment: Insights into Exosome-Mediated Cell Interactions DOI Creative Commons

Churan Wang,

Shun Xu,

Xiao Yang

и другие.

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 8211 - 8236

Опубликована: Авг. 1, 2024

Hypoxia, as a prominent feature of the tumor microenvironment, has profound impact on multicomponent changes within this environment. Under hypoxic conditions, malignant phenotype cells, variety cell types well intercellular communication and material exchange, undergo complex alterations. These provide significant prospects for exploring mechanisms development under different microenvironmental conditions devising therapeutic strategies. Exosomes secreted by cells stromal are integral components serving crucial mediators have consequently garnered increasing attention from researchers. This review focuses which promote release exosomes alter their encapsulated contents. It also examines effects derived immune other microenvironment. Additionally, we summarize current research progress potential clinical applications propose future directions in field.

Язык: Английский

Процитировано

8
Nucleic Acid Amplification Circuit‐Based Hydrogel (NACH) Assay for One‐Step Detection of Metastatic Gastric Cancer‐Derived Exosomal miRNA DOI Creative Commons

Seung Beom Seo,

Jaewoo Lim,

Kyujung Kim

и другие.

Advanced Science, Год журнала: 2024, Номер unknown

Опубликована: Сен. 23, 2024

Gastric cancer (GC) is recognized as the fifth most prevalent malignant tumor worldwide. It characterized by diverse clinical symptoms, treatment responses, and prognoses. In GC prognosis, promotion of epithelial-mesenchymal transition (EMT) fosters cell invasion metastasis, thereby triggering dissemination cells. This study proposes a nucleic acid amplification circuit-based hydrogel (NACH) assay for identifying exosomal miRNA derived from metastatic GC. The NACH employs rolling circle method targets miRNA-21, tumor-related oncogene, miRNA-99a, which promotes EMT. Specific probes each target are immobilized within hydrogel, enabling streamlined, one-step reaction. exhibits detection limit 1 fm miRNA-21 rapid highly sensitive on-site detection. Performance evaluation using extracted culture media, mouse plasma, human plasma revealed fluorescence intensity patterns similar to those obtained in qRT-PCR. Furthermore, deploying custom-developed portable fluorometer allows diagnostic performance assessment point-of-care testing samples patients. These findings emphasize potential be used robust tool genetic diagnosis based on exosome

Язык: Английский

Процитировано

7
Engineering of a Multi‐Modular DNA Nanodevice for Spatioselective Imaging and Evaluation of NK Cell‐Mediated Cancer Immunotherapy DOI

Zhao‐Peng Chen,

Weijia Zeng,

Yan‐Mei Lei

и другие.

Angewandte Chemie International Edition, Год журнала: 2024, Номер unknown

Опубликована: Окт. 8, 2024

Granzyme A (GzmA) secreted by natural killer (NK) cells has garnered considerable interest as a biomarker to evaluate the efficacy of cancer immunotherapy. However, current methodologies selectively monitor spatial distribution GzmA in during NK cell-targeted therapy are extremely challenging, primarily due existence diverse cell populations, low levels expression, and limited availability probes. Herein we develop multi-modular, structurally-ordered DNA nanodevice for evaluating cell-mediated immunotherapy (MODERN), that permits spatioselective imaging through GzmA-induced apurinic/apyrimidinic endonuclease 1 (APE1) inactivation. The MODERN incorporates multiple functional modules, including an APE1-gated recognition module, photo-activated amplification aptamer-mediated tumor-target polycatenane enabling improved sensitivity specificity towards intracellular GzmA. was activated (on) overexpression APE1, whereas it remained silent (off) NK-treated owing APE1 Furthermore, demonstrated inactivation blocks cellular repair target cells, resulting efficient death. This relies on specific should be beneficial

Язык: Английский

Процитировано

7
Supramolecular DNA Nanodevice Assembled via RCA and HCR Cascade Reaction on Tetrahedral DNA Nanostructure for Sensitive Detection and Intracellular Imaging of Dual-miRNAs Associated with Liver Cancer DOI

Xue-Mei Zhou,

Si–Yi Chen, Yaqin Chai

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Herein, a supramolecular DNA nanodevice was formed via the rolling circle amplification (RCA) and hybridization chain reaction (HCR) cascade on tetrahedral nanostructure (TDN) to achieve simultaneous sensitive detection intracellular imaging of dual-miRNAs related liver cancer. The effectively addressed limitations low probe loading capacity in traditional TDN nanodevices by enriching plenty signal probes around single TDN, significantly enhancing fluorescence signal. Impressively, with fulcrum dense structure imparted strong rigidity, ensuring stability decrease aggregation quenching for further increasing response. Consequently, biosensor based enabled miRNA221 miRNA222, achieved accurate situ displaying improved capabilities compared TDN-based nanodevices. More importantly, precise miRNA222 could distinguish hepatocellular carcinoma cells different degrees metastasis from human normal cells, providing more information diagnosis development carcinoma.

Язык: Английский

Процитировано

1
Harnessing crRNA Transformer for Facile and Specific Nucleic Acid Detection DOI
Mei Wen, Jinrong Min,

Zhaoxin Huang

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 5, 2025

CRISPR/Cas systems have emerged as promising tools for nucleic acid detection. However, their practical applications been limited by poor specificity and the need additional preprocessing steps. Inspired concept of transformers, we found that changing forms crRNA with spatial arrangement may endow an enhanced performance Specifically, rationally designed two transformers─swap split crRNA─and they direct system cis- trans- cleavage decreased Cas binding affinity possess both DNA RNA detection abilities. Based on these findings, our strategy enabled identification clinical prostatic cancer in a one-step reaction, remarkable sensitivity 90.0% 96.0%. Our study deepens understanding introduces simple specificity, sensitivity, functionality molecular diagnosis.

Язык: Английский

Процитировано

1
Recent progress and challenges in ECL biosensors for the identification of small extracellular vesicle and clinical diagnosis DOI
Shijie Li,

Peilin Wang,

Qiang Ma

и другие.

TrAC Trends in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 118253 - 118253

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

1
Interpretable Machine Learning-Aided Optical Deciphering of Serum Exosomes for Early Detection, Staging, and Subtyping of Lung Cancer DOI
Yujie Liu,

Chenlei Cai,

Weijie Xu

и другие.

Analytical Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Окт. 3, 2024

Lung cancer (LC) is the leading cause of cancer-related mortality worldwide, underscoring an urgent need for strategies that enable early detection and phenotypic classification. Here, we conducted a label-free surface-enhanced Raman spectroscopic (SERS) analysis serum exosomes from 643 participants to elucidate biochemical deregulation associated with LC progression unique phenotypes different subtypes. Iodide-modified silver nanofilms were prepared rapidly acquire SERS spectra high signal-to-noise ratio using 0.5 μL patient exosomes. We performed interpretable automated machine learning (ML) differential features build diagnostic models, which achieved accuracies 100% 81% stage I lung adenocarcinoma its preneoplasia, respectively. In addition, ML-derived exosomal models effectively recognized subtypes disease stages guide precision treatment. Our findings demonstrate spectral fingerprinting circulating holds promise decoding clinical status LC, thus aiding in improving management patients.

Язык: Английский

Процитировано

6