Chemical Communications, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Extracellular vesicles have been deemed as potential drug carriers for treatment of various diseases. Recent advances summarized, including the sources, delivery function, extraction and cargo-loading technology extracellular vesicles.
Язык: Английский
Translational Oncology, Год журнала: 2024, Номер 50, С. 102121 - 102121
Опубликована: Сен. 14, 2024
Язык: Английский
Процитировано
60
Advanced Science, Год журнала: 2024, Номер 11(30)
Опубликована: Июнь 14, 2024
Abstract In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the life sciences due to their critical roles both health and disease states, offering promising applications diagnosis, drug delivery, therapy. However, inherent heterogeneity complex origins pose significant challenges preparation, analysis, subsequent clinical application. This review is structured provide an overview of biogenesis, composition, various sources EVs, thereby laying groundwork for a detailed discussion contemporary techniques preparation analysis. Particular focus given state‐of‐the‐art technologies that employ microfluidic non‐microfluidic platforms EV processing. Furthermore, this discourse extends into innovative approaches incorporate artificial intelligence cutting‐edge electrochemical sensors, with particular emphasis single proposes current outlines prospective avenues future research. The objective motivate researchers innovate expand methods analysis fully unlocking biomedical potential.
Язык: Английский
Процитировано
41
Nano Convergence, Год журнала: 2024, Номер 11(1)
Опубликована: Май 29, 2024
Abstract Liquid biopsy has emerged as a promising non-invasive strategy for cancer diagnosis, enabling the detection of various circulating biomarkers, including tumor cells (CTCs), nucleic acids (ctNAs), tumor-derived small extracellular vesicles (sEVs), and proteins. Surface-enhanced Raman scattering (SERS) biosensors have revolutionized liquid by offering sensitive specific methodologies these biomarkers. This review comprehensively examines application SERS-based identification analysis biomarkers CTCs, ctNAs, sEVs proteins in diagnosis. The discussion encompasses diverse range SERS biosensor platforms, label-free assay, magnetic bead-based microfluidic device-based system, paper-based each demonstrating unique capabilities enhancing sensitivity specificity critically assesses strengths, limitations, future directions
Язык: Английский
Процитировано
30
Lab on a Chip, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
This review highlights recent technological advances for progress in particle manipulation under X-force fields, and forecasts the trajectory of future developments.
Язык: Английский
Процитировано
2
Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)
Опубликована: Окт. 8, 2024
Liquid biopsy is a minimally invasive method that uses biofluid samples instead of tissue for cancer diagnosis. Exosomes are small extracellular vesicles secreted by donor cells and act as mediators intercellular communication in human health disease. Due to their important roles, exosomes have been considered promising biomarkers liquid biopsy. However, traditional methods exosome isolation cargo detection time-consuming inefficient, limiting practical application. In the past decades, many new strategies, such microfluidic chips, nanowire arrays electrochemical biosensors, proposed achieve rapid, accurate high-throughput analysis exosomes. this review, we discussed about advance exosome-based technology, including isolation, enrichment, approaches. The comparison currently available also included. Finally, summarized advantages limitations present strategies further gave perspective future translational use.
Язык: Английский
Процитировано
10
Journal of Controlled Release, Год журнала: 2025, Номер 379, С. 489 - 523
Опубликована: Янв. 18, 2025
Язык: Английский
Процитировано
1
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Фев. 25, 2025
Mass spectrometry-based proteomics is about 35 years old, and recent progress appears to be speeding up across all subfields. In this review, we focus on advances over the last two in select areas within bottom-up proteomics, including approaches high-throughput experiments, data analysis using machine learning, drug discovery, glycoproteomics, extracellular vesicle structural proteomics.
Язык: Английский
Процитировано
1
ACS Nano, Год журнала: 2025, Номер unknown
Опубликована: Март 9, 2025
Neutrophil-derived extracellular vesicles (NEVs) are critically involved in disease progression and considered potential biomarkers. However, the tedious processes of NEV separation detection restrain their use. Herein, we presented an integrated microfluidic chip for (IMCN) analysis, which achieved immune-separation CD66b+ NEVs multiplexed contained miRNAs (termed signatures) by using 10 μL serum samples. The optimized microchannel flow rate IMCN enabled efficient capture (>90%). After recognition captured a specific CD63 aptamer, on-chip rolling circle amplification (RCA) reaction was triggered released aptamers from heat-lysed NEVs. Then, RCA products bound to molecular beacons (MBs), initiating allosteric hairpin structures amplified "turn on" fluorescence signals (RCA-MB assay). Clinical sample analysis showed that signatures had high area under curve (AUC) distinguishing between healthy control (HC) gastric cancer (GC) (0.891), benign diseases (BGD) GC (0.857). Notably, AUC reached 0.912 with combination five biomarkers (NEV signatures, CEA, CA199) differentiate HC, diagnostic accuracy further increased machine learning (ML)-based ensemble classification system. Therefore, developed is valuable platform may have use diagnosis.
Язык: Английский
Процитировано
1
Science Advances, Год журнала: 2025, Номер 11(16)
Опубликована: Апрель 16, 2025
Exosomes secreted by cells hold substantial potential for disease diagnosis and treatment. However, the rapid isolation of high-purity exosomes their subpopulations from biofluids (e.g., undiluted whole blood) remains challenging. This study presents oscillating microbubble array–based metamaterials (OMAMs) enabling without labeling or preprocessing. Particularly, leveraging acoustically excited oscillation, OMAMs can generate numerous acoustofluidic traps filtering in-fluid micro/nanoparticles, thus allowing removing bioparticles larger than to obtain (93%) blood in ~3 minutes. Moreover, exosome different size ranges be isolated tuning oscillation amplitude. Additionally, as each functions an ultradeep subwavelength (~λ/186) acoustic amplifier a nonlinear source, high-resolution complex energy patterns tune activating different-sized microbubbles at distinct resonance frequencies.
Язык: Английский
Процитировано
1
Journal of Molecular Biology, Год журнала: 2025, Номер unknown, С. 169218 - 169218
Опубликована: Май 1, 2025
Язык: Английский
Процитировано
1