An updated review of HSV-1 infection-associated diseases and treatment, vaccine development, and vector therapy application

Virulence, Год журнала: 2024, Номер 15(1)

Опубликована: Ноя. 7, 2024

Herpes simplex virus type 1 (HSV-1) is a globally widespread that causes and associates with wide range of diseases, including herpes encephalitis, keratitis, labialis. The interaction between HSV-1 the host involves complex immune response mechanisms, recognition viral invasion, maintenance latent infection, triggering reactivation. Antiviral therapy core treatment for infections. Meanwhile, vaccine development employs different strategies methods, several promising types have emerged, such as live attenuated, protein subunit, nucleic acid vaccines, offering new possibilities prevention infection. Moreover, can be modified into therapeutic vector gene tumour immunotherapy. This review provides an in-depth summary infection-associated innate adaptive responses, disease pathogenesis, current approaches, recent advances in development, applications cancer treatment. Through systematic multiple aspects HSV-1, this study aims to provide comprehensive detailed reference public on prevention, control, HSV-1.

Язык: Английский

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Serum starvation impacts rhinovirus spread from cell to cell

Virology, Год журнала: 2025, Номер 604, С. 110408 - 110408

Опубликована: Янв. 20, 2025

Язык: Английский

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The producer cell type of HSV-1 alters the proteomic contents and infectious capacity of virions

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 4, 2025

The cell that a virus replicates in i.e., the producer cell, can alter macromolecular composition and infectious capacity of virions are produced. Herpes Simplex type 1 (HSV-1) primarily infects keratinocytes epidermis or oral mucosa prior to establishing latency neurons peripheral nervous system, where persist for lifetime host. Many lines used amplify HSV-1 derived from species tissue types less physiologically relevant disease. To understand if influences infection, we tested infectivity immortalized African green monkey kidney cells (vero), human (HaCaT), primary foreskin fibroblasts (HFF-1). We observed alters produce viral proteins infected susceptibility inhibition replication by interferon treatment. HaCaT-derived consistently exhibited enhanced over HFF-1 vero-derived virus. determine changes protein virions, performed an untargeted LC/MS-MS analysis purified each line. Comparison virion associated revealed quantitative differences both cellular including ICP0, pUL24 pUL42. These results highlight influence cell-type has on infection outcomes suggest specific factors impact replication. Approximately 67% population harbors infection. study laboratories utilize several different propagate downstream experiments. virus, i.e. type, composition, immunogenicity, produced across families. found composition. Therefore, may have implications spread subsequent disease humans. Our also raise concerns about how use ceil outcome, interpretation, reproducibility experimental results.

Язык: Английский

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A dual fluorescent herpes simplex virus type 1 recombinant reveals divergent outcomes of neuronal infection

Journal of Virology, Год журнала: 2024, Номер 98(5)

Опубликована: Апрель 23, 2024

Critical stages of lytic herpes simplex virus type 1 (HSV-1) replication are marked by the sequential expression immediate early (IE) to (E), then late (L) viral genes. HSV-1 can also persist in neuronal cells via a non-replicative, transcriptionally repressed infection called latency. The regulation and latent transcriptional profiles is critical pathogenesis persistence. We sought fluorescence-based approach observe outcome at single-cell level. To achieve this goal, we constructed characterized novel recombinant that enables discrimination between infection. dual reporter encodes human cytomegalovirus-immediate (hCMV-IE) promoter-driven enhanced yellow fluorescent protein (eYFP) visualize establishment an endogenous mCherry-VP26 fusion report replication. confirmed gene expression, replication, spread not altered incorporation reporters, (FP) detection virtuously reports progression demonstrate compartmentalized primary neurons determined inoculating dose: high-dose axonal inoculation proceeds whereas low-dose establishes Interfering with small molecule drugs divergent phenotypes eYFP mCherry detection, correlating states expression. state variable response changes intracellular environment.IMPORTANCEHerpes prevalent pathogen infects approximately 67% global population. invades peripheral nervous system, where persists within host for life. Immunological evasion, persistence, herpetic pathologies Studying during challenging but essential development antiviral therapeutics interventions. used evaluate neurons. Manipulation cell signaling pathways impacts latency work here provides insight into cellular factors contributing

Язык: Английский

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Advances in virus-host interaction research based on microfluidic platforms

Chinese Chemical Letters, Год журнала: 2024, Номер 35(12), С. 110302 - 110302

Опубликована: Июль 31, 2024

Язык: Английский

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All the single cells: if you like it then you should put some virus on it

Journal of Virology, Год журнала: 2024, Номер 98(7)

Опубликована: Июнь 21, 2024

Across a rich 70-year history, single-cell virology has revealed the impact of host and pathogen heterogeneity during virus infections. Recent technological innovations have enabled higher-resolution analyses cellular viral heterogeneity. Furthermore, analysis extreme phenotypes provided additional insights into host-pathogen dynamics. Using approach to explore fundamental questions, contemporary researchers contributed revival interest in with increased enthusiasm.

Язык: Английский

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Development of a reporter feline herpesvirus-1 for antiviral screening assays

Veterinary Research, Год журнала: 2024, Номер 55(1)

Опубликована: Дек. 18, 2024

Язык: Английский

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