Tumor‐derived spheroids: Relevance to cancer stem cells and clinical applications DOI Creative Commons
Tatsuya Ishiguro,

Hirokazu Ohata,

Ai Sato

и другие.

Cancer Science, Год журнала: 2017, Номер 108(3), С. 283 - 289

Опубликована: Янв. 8, 2017

Recently, many types of in vitro 3-D culture systems have been developed to recapitulate the vivo growth conditions cancer. The cancer methods aim preserve biological characteristics original tumors better than conventional 2-D monolayer cultures, and include tumor-derived organoids, spheroids, organotypic multicellular tumor spheroids. differ terms cell sources, protocols for handling, required time intervals. Tumor-derived spheroids are unique because they purposed enrichment stem cells (CSCs) or with cell-related characteristics. These grown as floating spheres used surrogate evaluate CSC-related solid vitro. Because eradication CSCs is likely be clinical importance due their association malignant nature cells, such tumorigenicity chemoresistance, investigation may provide invaluable clues fight against Spheroid cultures established from cancers including glioma, breast, colon, ovary, prostate cancers, biochemical investigated by research groups. In addition CSCs, prove instrumental a high-throughput screening platform cultivation found circulation body fluids.

Язык: Английский

Emerging Biological Principles of Metastasis DOI Creative Commons
Arthur W. Lambert, Diwakar R. Pattabiraman, Robert A. Weinberg

и другие.

Cell, Год журнала: 2017, Номер 168(4), С. 670 - 691

Опубликована: Фев. 1, 2017

Язык: Английский

Процитировано

2679

Integrating liquid biopsies into the management of cancer DOI
Giulia Siravegna, Silvia Marsoni, Salvatore Siena

и другие.

Nature Reviews Clinical Oncology, Год журнала: 2017, Номер 14(9), С. 531 - 548

Опубликована: Март 2, 2017

Язык: Английский

Процитировано

1646

Current and future perspectives of liquid biopsies in genomics-driven oncology DOI
Ellen Heitzer, Imran S. Haque,

Charles E. S. Roberts

и другие.

Nature Reviews Genetics, Год журнала: 2018, Номер 20(2), С. 71 - 88

Опубликована: Ноя. 8, 2018

Язык: Английский

Процитировано

1176

Oxidative stress inhibits distant metastasis by human melanoma cells DOI
Elena Piskounova, Michalis Agathocleous, Malea M. Murphy

и другие.

Nature, Год журнала: 2015, Номер 527(7577), С. 186 - 191

Опубликована: Окт. 13, 2015

Язык: Английский

Процитировано

1124

Spherical Cancer Models in Tumor Biology DOI Creative Commons
Louis‐Bastien Weiswald,

Dominique Bellet,

Virginie Dangles-Marie

и другие.

Neoplasia, Год журнала: 2015, Номер 17(1), С. 1 - 15

Опубликована: Янв. 1, 2015

Three-dimensional (3D) in vitro models have been used cancer research as an intermediate model between cell line cultures and vivo tumor.Spherical represent major 3D that described over the past 4 decades.These gained popularity stem using tumorospheres.Thus, it is crucial to define clarify different spherical thus far described.Here, we focus on multicellular spheres research.All these spherelike structures are characterized by their well-rounded shape, presence of cells, capacity be maintained free-floating cultures.We propose a rational classification four most commonly based culture methods for obtaining them subsequent differences sphere biology: tumor spheroid model, first early 70s obtained lines under nonadherent conditions; tumorospheres, expansion established serum-free medium supplemented with growth factors; tissue-derived organotypic spheroids, tissue mechanical dissociation cutting.In addition, describe applications interest research; particular, contribution chemoresistance, radioresistance, tumorigenicity, invasion migration studies.Although share common conformation, each displays its own intrinsic properties.Therefore, relevant must carefully selected, function study aim type.

Язык: Английский

Процитировано

1030

Neutrophils escort circulating tumour cells to enable cell cycle progression DOI

Barbara M. Szczerba,

Francesc Castro-Giner, Marcus Vetter

и другие.

Nature, Год журнала: 2019, Номер 566(7745), С. 553 - 557

Опубликована: Фев. 6, 2019

Язык: Английский

Процитировано

1001

Liquid biopsy enters the clinic — implementation issues and future challenges DOI
Michail Ignatiadis, George W. Sledge, Stefanie S. Jeffrey

и другие.

Nature Reviews Clinical Oncology, Год журнала: 2021, Номер 18(5), С. 297 - 312

Опубликована: Янв. 20, 2021

Язык: Английский

Процитировано

878

Liquid biopsy and minimal residual disease — latest advances and implications for cure DOI
Klaus Pantel, Catherine Alix‐Panabières

Nature Reviews Clinical Oncology, Год журнала: 2019, Номер 16(7), С. 409 - 424

Опубликована: Фев. 22, 2019

Язык: Английский

Процитировано

866

Acoustic separation of circulating tumor cells DOI Open Access
Peng Li,

Zhangming Mao,

Zhangli Peng

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2015, Номер 112(16), С. 4970 - 4975

Опубликована: Апрель 6, 2015

Significance The separation and analysis of circulating tumor cells (CTCs) provides physicians a minimally invasive way to monitor the response cancer patients various treatments. Among existing cell-separation methods, acoustic-based approaches provide significant potential preserve phenotypic genotypic characteristics sorted cells, owing their safe, label-free, contactless nature. In this work, we report development an device that successfully demonstrates isolation rare CTCs from clinical blood samples patients. Our work thus unique means obtain viable undamaged CTCs, which can subsequently be cultured. results presented here offer pathways for better diagnosis, prognosis, therapy monitoring, metastasis research.

Язык: Английский

Процитировано

723

Circulating Tumor Cell Clustering Shapes DNA Methylation to Enable Metastasis Seeding DOI Creative Commons

Sofia Gkountela,

Francesc Castro-Giner,

Barbara M. Szczerba

и другие.

Cell, Год журнала: 2019, Номер 176(1-2), С. 98 - 112.e14

Опубликована: Янв. 1, 2019

The ability of circulating tumor cells (CTCs) to form clusters has been linked increased metastatic potential. Yet biological features and vulnerabilities CTC remain largely unknown. Here, we profile the DNA methylation landscape single CTCs from breast cancer patients mouse models on a genome-wide scale. We find that binding sites for stemness- proliferation-associated transcription factors are specifically hypomethylated in clusters, including OCT4, NANOG, SOX2, SIN3A, paralleling embryonic stem cell biology. Among 2,486 FDA-approved compounds, identify Na+/K+ ATPase inhibitors enable dissociation into cells, leading remodeling at critical metastasis suppression. Thus, our results link clustering specific changes promote stemness point cluster-targeting compounds suppress spread cancer.

Язык: Английский

Процитировано

719