Cancer Science,
Год журнала:
2017,
Номер
108(3), С. 283 - 289
Опубликована: Янв. 8, 2017
Recently,
many
types
of
in
vitro
3-D
culture
systems
have
been
developed
to
recapitulate
the
vivo
growth
conditions
cancer.
The
cancer
methods
aim
preserve
biological
characteristics
original
tumors
better
than
conventional
2-D
monolayer
cultures,
and
include
tumor-derived
organoids,
spheroids,
organotypic
multicellular
tumor
spheroids.
differ
terms
cell
sources,
protocols
for
handling,
required
time
intervals.
Tumor-derived
spheroids
are
unique
because
they
purposed
enrichment
stem
cells
(CSCs)
or
with
cell-related
characteristics.
These
grown
as
floating
spheres
used
surrogate
evaluate
CSC-related
solid
vitro.
Because
eradication
CSCs
is
likely
be
clinical
importance
due
their
association
malignant
nature
cells,
such
tumorigenicity
chemoresistance,
investigation
may
provide
invaluable
clues
fight
against
Spheroid
cultures
established
from
cancers
including
glioma,
breast,
colon,
ovary,
prostate
cancers,
biochemical
investigated
by
research
groups.
In
addition
CSCs,
prove
instrumental
a
high-throughput
screening
platform
cultivation
found
circulation
body
fluids.
Neoplasia,
Год журнала:
2015,
Номер
17(1), С. 1 - 15
Опубликована: Янв. 1, 2015
Three-dimensional
(3D)
in
vitro
models
have
been
used
cancer
research
as
an
intermediate
model
between
cell
line
cultures
and
vivo
tumor.Spherical
represent
major
3D
that
described
over
the
past
4
decades.These
gained
popularity
stem
using
tumorospheres.Thus,
it
is
crucial
to
define
clarify
different
spherical
thus
far
described.Here,
we
focus
on
multicellular
spheres
research.All
these
spherelike
structures
are
characterized
by
their
well-rounded
shape,
presence
of
cells,
capacity
be
maintained
free-floating
cultures.We
propose
a
rational
classification
four
most
commonly
based
culture
methods
for
obtaining
them
subsequent
differences
sphere
biology:
tumor
spheroid
model,
first
early
70s
obtained
lines
under
nonadherent
conditions;
tumorospheres,
expansion
established
serum-free
medium
supplemented
with
growth
factors;
tissue-derived
organotypic
spheroids,
tissue
mechanical
dissociation
cutting.In
addition,
describe
applications
interest
research;
particular,
contribution
chemoresistance,
radioresistance,
tumorigenicity,
invasion
migration
studies.Although
share
common
conformation,
each
displays
its
own
intrinsic
properties.Therefore,
relevant
must
carefully
selected,
function
study
aim
type.
Proceedings of the National Academy of Sciences,
Год журнала:
2015,
Номер
112(16), С. 4970 - 4975
Опубликована: Апрель 6, 2015
Significance
The
separation
and
analysis
of
circulating
tumor
cells
(CTCs)
provides
physicians
a
minimally
invasive
way
to
monitor
the
response
cancer
patients
various
treatments.
Among
existing
cell-separation
methods,
acoustic-based
approaches
provide
significant
potential
preserve
phenotypic
genotypic
characteristics
sorted
cells,
owing
their
safe,
label-free,
contactless
nature.
In
this
work,
we
report
development
an
device
that
successfully
demonstrates
isolation
rare
CTCs
from
clinical
blood
samples
patients.
Our
work
thus
unique
means
obtain
viable
undamaged
CTCs,
which
can
subsequently
be
cultured.
results
presented
here
offer
pathways
for
better
diagnosis,
prognosis,
therapy
monitoring,
metastasis
research.
Cell,
Год журнала:
2019,
Номер
176(1-2), С. 98 - 112.e14
Опубликована: Янв. 1, 2019
The
ability
of
circulating
tumor
cells
(CTCs)
to
form
clusters
has
been
linked
increased
metastatic
potential.
Yet
biological
features
and
vulnerabilities
CTC
remain
largely
unknown.
Here,
we
profile
the
DNA
methylation
landscape
single
CTCs
from
breast
cancer
patients
mouse
models
on
a
genome-wide
scale.
We
find
that
binding
sites
for
stemness-
proliferation-associated
transcription
factors
are
specifically
hypomethylated
in
clusters,
including
OCT4,
NANOG,
SOX2,
SIN3A,
paralleling
embryonic
stem
cell
biology.
Among
2,486
FDA-approved
compounds,
identify
Na+/K+
ATPase
inhibitors
enable
dissociation
into
cells,
leading
remodeling
at
critical
metastasis
suppression.
Thus,
our
results
link
clustering
specific
changes
promote
stemness
point
cluster-targeting
compounds
suppress
spread
cancer.
