Tumor‐derived spheroids: Relevance to cancer stem cells and clinical applications DOI Creative Commons
Tatsuya Ishiguro,

Hirokazu Ohata,

Ai Sato

et al.

Cancer Science, Journal Year: 2017, Volume and Issue: 108(3), P. 283 - 289

Published: Jan. 8, 2017

Recently, many types of in vitro 3-D culture systems have been developed to recapitulate the vivo growth conditions cancer. The cancer methods aim preserve biological characteristics original tumors better than conventional 2-D monolayer cultures, and include tumor-derived organoids, spheroids, organotypic multicellular tumor spheroids. differ terms cell sources, protocols for handling, required time intervals. Tumor-derived spheroids are unique because they purposed enrichment stem cells (CSCs) or with cell-related characteristics. These grown as floating spheres used surrogate evaluate CSC-related solid vitro. Because eradication CSCs is likely be clinical importance due their association malignant nature cells, such tumorigenicity chemoresistance, investigation may provide invaluable clues fight against Spheroid cultures established from cancers including glioma, breast, colon, ovary, prostate cancers, biochemical investigated by research groups. In addition CSCs, prove instrumental a high-throughput screening platform cultivation found circulation body fluids.

Language: Английский

Emerging Biological Principles of Metastasis DOI Creative Commons
Arthur W. Lambert, Diwakar R. Pattabiraman, Robert A. Weinberg

et al.

Cell, Journal Year: 2017, Volume and Issue: 168(4), P. 670 - 691

Published: Feb. 1, 2017

Language: Английский

Citations

2679
Integrating liquid biopsies into the management of cancer DOI
Giulia Siravegna, Silvia Marsoni, Salvatore Siena

et al.

Nature Reviews Clinical Oncology, Journal Year: 2017, Volume and Issue: 14(9), P. 531 - 548

Published: March 2, 2017

Language: Английский

Citations

1646
Current and future perspectives of liquid biopsies in genomics-driven oncology DOI
Ellen Heitzer, Imran S. Haque,

Charles E. S. Roberts

et al.

Nature Reviews Genetics, Journal Year: 2018, Volume and Issue: 20(2), P. 71 - 88

Published: Nov. 8, 2018

Language: Английский

Citations

1173
Oxidative stress inhibits distant metastasis by human melanoma cells DOI
Elena Piskounova, Michalis Agathocleous, Malea M. Murphy

et al.

Nature, Journal Year: 2015, Volume and Issue: 527(7577), P. 186 - 191

Published: Oct. 13, 2015

Language: Английский

Citations

1124
Spherical Cancer Models in Tumor Biology DOI Creative Commons
Louis‐Bastien Weiswald,

Dominique Bellet,

Virginie Dangles-Marie

et al.

Neoplasia, Journal Year: 2015, Volume and Issue: 17(1), P. 1 - 15

Published: Jan. 1, 2015

Three-dimensional (3D) in vitro models have been used cancer research as an intermediate model between cell line cultures and vivo tumor.Spherical represent major 3D that described over the past 4 decades.These gained popularity stem using tumorospheres.Thus, it is crucial to define clarify different spherical thus far described.Here, we focus on multicellular spheres research.All these spherelike structures are characterized by their well-rounded shape, presence of cells, capacity be maintained free-floating cultures.We propose a rational classification four most commonly based culture methods for obtaining them subsequent differences sphere biology: tumor spheroid model, first early 70s obtained lines under nonadherent conditions; tumorospheres, expansion established serum-free medium supplemented with growth factors; tissue-derived organotypic spheroids, tissue mechanical dissociation cutting.In addition, describe applications interest research; particular, contribution chemoresistance, radioresistance, tumorigenicity, invasion migration studies.Although share common conformation, each displays its own intrinsic properties.Therefore, relevant must carefully selected, function study aim type.

Language: Английский

Citations

1030
Neutrophils escort circulating tumour cells to enable cell cycle progression DOI

Barbara M. Szczerba,

Francesc Castro-Giner, Marcus Vetter

et al.

Nature, Journal Year: 2019, Volume and Issue: 566(7745), P. 553 - 557

Published: Feb. 6, 2019

Language: Английский

Citations

1001
Liquid biopsy enters the clinic — implementation issues and future challenges DOI
Michail Ignatiadis, George W. Sledge, Stefanie S. Jeffrey

et al.

Nature Reviews Clinical Oncology, Journal Year: 2021, Volume and Issue: 18(5), P. 297 - 312

Published: Jan. 20, 2021

Language: Английский

Citations

878
Liquid biopsy and minimal residual disease — latest advances and implications for cure DOI
Klaus Pantel, Catherine Alix‐Panabières

Nature Reviews Clinical Oncology, Journal Year: 2019, Volume and Issue: 16(7), P. 409 - 424

Published: Feb. 22, 2019

Language: Английский

Citations

865
Acoustic separation of circulating tumor cells DOI Open Access
Peng Li,

Zhangming Mao,

Zhangli Peng

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2015, Volume and Issue: 112(16), P. 4970 - 4975

Published: April 6, 2015

Significance The separation and analysis of circulating tumor cells (CTCs) provides physicians a minimally invasive way to monitor the response cancer patients various treatments. Among existing cell-separation methods, acoustic-based approaches provide significant potential preserve phenotypic genotypic characteristics sorted cells, owing their safe, label-free, contactless nature. In this work, we report development an device that successfully demonstrates isolation rare CTCs from clinical blood samples patients. Our work thus unique means obtain viable undamaged CTCs, which can subsequently be cultured. results presented here offer pathways for better diagnosis, prognosis, therapy monitoring, metastasis research.

Language: Английский

Citations

723
Circulating Tumor Cell Clustering Shapes DNA Methylation to Enable Metastasis Seeding DOI Creative Commons

Sofia Gkountela,

Francesc Castro-Giner,

Barbara M. Szczerba

et al.

Cell, Journal Year: 2019, Volume and Issue: 176(1-2), P. 98 - 112.e14

Published: Jan. 1, 2019

The ability of circulating tumor cells (CTCs) to form clusters has been linked increased metastatic potential. Yet biological features and vulnerabilities CTC remain largely unknown. Here, we profile the DNA methylation landscape single CTCs from breast cancer patients mouse models on a genome-wide scale. We find that binding sites for stemness- proliferation-associated transcription factors are specifically hypomethylated in clusters, including OCT4, NANOG, SOX2, SIN3A, paralleling embryonic stem cell biology. Among 2,486 FDA-approved compounds, identify Na+/K+ ATPase inhibitors enable dissociation into cells, leading remodeling at critical metastasis suppression. Thus, our results link clustering specific changes promote stemness point cluster-targeting compounds suppress spread cancer.

Language: Английский

Citations

719