Nucleic Acids Research,
Год журнала:
2020,
Номер
48(20), С. 11380 - 11393
Опубликована: Окт. 16, 2020
Advancing
the
molecular
knowledge
surrounding
fertility
and
inheritance
has
become
critical
given
halving
of
sperm
counts
in
last
40
years,
rise
complex
disease
which
cannot
be
explained
by
genetics
alone.
The
connection
between
both
these
trends
may
lie
alterations
to
epigenome
occur
through
environmental
exposures.
Changes
are
also
associated
with
health
risks
across
generations
such
as
metabolic
disorders
cancer.
Thus,
it
is
imperative
identify
epigenetic
modifications
that
escape
reprogramming
during
spermatogenesis
embryogenesis.
Here,
we
aimed
chromatin
signature(s)
involved
transgenerational
phenotypes
our
genetic
mouse
model
overexpresses
histone
demethylase
KDM1A
their
germ
cells.
We
used
sperm-specific
immunoprecipitation
followed
depth
sequencing
(ChIP-seq),
computational
analysis
whether
differential
enrichment
H3
lysine
4
trimethylation
(H3K4me3),
27
(H3K27me3)
serve
mechanisms
for
paternal
germline.
Our
on
transgenic
males
revealed
specific
changes
H3K4me3
predominantly
occurred
independently
from
bivalent
H3K4me3/H3K27me3
regions.
Many
regions
altered
were
identified
allele
pre-implantation
embryo.
These
findings
suggest
functions
transmission
non-genetic
transgenerationally.
Cell Research,
Год журнала:
2019,
Номер
29(5), С. 379 - 390
Опубликована: Фев. 18, 2019
Global
warming
has
profound
effects
on
plant
growth
and
fitness.
Plants
have
evolved
sophisticated
epigenetic
machinery
to
respond
quickly
heat,
exhibit
transgenerational
memory
of
the
heat-induced
release
post-transcriptional
gene
silencing
(PTGS).
However,
how
thermomemory
is
transmitted
progeny
physiological
relevance
are
elusive.
Here
we
show
that
HEAT
SHOCK
TRANSCRIPTION
FACTOR
A2
(HSFA2)
directly
activates
H3K27me3
demethylase
RELATIVE
OF
EARLY
FLOWERING
6
(REF6),
which
in
turn
derepresses
HSFA2.
REF6
HSFA2
establish
a
heritable
feedback
loop,
activate
an
E3
ubiquitin
ligase,
SUPPRESSOR
GENE
SILENCING
3
(SGS3)-INTERACTING
PROTEIN
1
(SGIP1).
SGIP1-mediated
SGS3
degradation
leads
inhibited
biosynthesis
trans-acting
siRNA
(tasiRNA).
The
REF6-HSFA2
loop
reduced
tasiRNA
converge
HEAT-INDUCED
TAS1
TARGET
5
(HTT5),
drives
early
flowering
but
attenuates
immunity.
Thus,
heat
induces
phenotypes
via
coordinated
network
involving
histone
demethylases,
transcription
factors,
tasiRNAs,
ensuring
reproductive
success
stress
adaptation.
Science,
Год журнала:
2018,
Номер
361(6409), С. 1332 - 1336
Опубликована: Сен. 28, 2018
During
development
and
throughout
life,
a
variety
of
specialized
cells
must
be
generated
to
ensure
the
proper
function
each
tissue
organ.
Chromatin
plays
key
role
in
determining
cellular
state,
whether
totipotent,
pluripotent,
multipotent,
or
differentiated.
We
highlight
chromatin
dynamics
involved
generation
pluripotent
stem
as
well
their
influence
on
cell
fate
decision
reprogramming.
focus
capacity
histone
variants,
chaperones,
modifications,
heterochromatin
factors
identity
its
plasticity.
Recent
technological
advances
have
provided
tools
elucidate
underlying
for
better
understanding
normal
pathological
conditions,
with
avenues
potential
therapeutic
application.
Protein & Cell,
Год журнала:
2020,
Номер
12(1), С. 7 - 28
Опубликована: Июль 15, 2020
Mammalian
fertilization
begins
with
the
fusion
of
two
specialized
gametes,
followed
by
major
epigenetic
remodeling
leading
to
formation
a
totipotent
embryo.
During
development
pre-implantation
embryo,
precise
reprogramming
progress
is
prerequisite
for
avoiding
developmental
defects
or
embryonic
lethality,
but
underlying
molecular
mechanisms
remain
elusive.
For
past
few
years,
unprecedented
breakthroughs
have
been
made
in
mapping
regulatory
network
dynamic
epigenomes
during
mammalian
early
embryo
development,
taking
advantage
multiple
advances
and
innovations
low-input
genome-wide
chromatin
analysis
technologies.
The
aim
this
review
highlight
most
recent
understanding
embryogenesis
mammals,
including
DNA
methylation,
histone
modifications,
accessibility
3D
organization.
Nature,
Год журнала:
2021,
Номер
593(7858), С. 289 - 293
Опубликована: Апрель 14, 2021
Abstract
Fundamental
features
of
3D
genome
organization
are
established
de
novo
in
the
early
embryo,
including
clustering
pericentromeric
regions,
folding
chromosome
arms
and
segregation
chromosomes
into
active
(A-)
inactive
(B-)
compartments.
However,
molecular
mechanisms
that
drive
remain
unknown
1,2
.
Here,
by
combining
conformation
capture
(Hi-C),
chromatin
immunoprecipitation
with
high-throughput
sequencing
(ChIP–seq),
DNA
fluorescence
situ
hybridization
(3D
FISH)
polymer
simulations,
we
show
heterochromatin
protein
1a
(HP1a)
is
essential
for
during
Drosophila
development.
The
binding
HP1a
at
required
to
establish
regions.
Moreover,
within
responsible
overall
has
an
important
role
formation
B-compartment
depletion
does
not
affect
A-compartment,
which
suggests
a
different
mechanism
segregates
Our
work
identifies
as
epigenetic
regulator
involved
establishing
global
structure
embryo.
Abstract
Epigenome
reprogramming
after
fertilization
enables
transcriptionally
quiescent
maternal
and
paternal
chromatin
to
acquire
a
permissive
state
for
subsequent
zygotic
genome
activation
(ZGA).
H3K27
acetylation
(H3K27ac)
is
well‐established
marker
of
active
enhancers
promoters.
However,
dynamics
H3K27ac
during
maternal‐to‐zygotic
transition
(MZT)
in
mammalian
embryos
are
not
well‐studied.
By
profiling
the
allelic
landscape
mouse
MZT,
we
show
that
undergoes
three
waves
rapid
global
transitions
between
oocyte
stage
2‐cell
stage.
Notably,
germinal
vesicle
zygote
globally
hyperacetylated,
with
noncanonical,
broad
domains
correlate
H3K4
trimethylation
(H3K4me3)
open
chromatin.
marks
genomic
regions
primed
including
ZGA
genes,
retrotransposons,
alleles
imprinted
genes.
We
CBP/p300
HDAC
activities
play
important
roles
regulating
essential
preimplantation
development.
Specifically,
acetyltransferase
broadly
deposits
zygotes
induce
opening
condensed
at
putative
ensure
proper
ZGA.
On
contrary,
HDACs
revert
canonical
safeguard
by
preventing
premature
expression
developmental
In
conclusion,
coordinated
MZT
