Transcription Factors Activate Genes through the Phase-Separation Capacity of Their Activation Domains

Cell, Год журнала: 2018, Номер 175(7), С. 1842 - 1855.e16

Опубликована: Ноя. 15, 2018

Язык: Английский

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Mediator and RNA polymerase II clusters associate in transcription-dependent condensates

Science, Год журнала: 2018, Номер 361(6400), С. 412 - 415

Опубликована: Июнь 21, 2018

Models of gene control have emerged from genetic and biochemical studies, with limited consideration the spatial organization dynamics key components in living cells. We used live-cell superresolution light-sheet imaging to study Mediator coactivator RNA polymerase II (Pol II) directly. Pol each form small transient large stable clusters embryonic stem are colocalized clusters, which associate chromatin, properties phase-separated condensates, sensitive transcriptional inhibitors. suggest that Mediator, recruited by transcription factors at or clustered enhancer elements, interact condensates vivo.

Язык: Английский

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Long non-coding RNAs: definitions, functions, challenges and recommendations

Nature Reviews Molecular Cell Biology, Год журнала: 2023, Номер 24(6), С. 430 - 447

Опубликована: Янв. 3, 2023

Язык: Английский

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Long-range enhancer–promoter contacts in gene expression control

Nature Reviews Genetics, Год журнала: 2019, Номер 20(8), С. 437 - 455

Опубликована: Май 13, 2019

Язык: Английский

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Organization of Chromatin by Intrinsic and Regulated Phase Separation

Cell, Год журнала: 2019, Номер 179(2), С. 470 - 484.e21

Опубликована: Сен. 19, 2019

Язык: Английский

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Physical Principles Underlying the Complex Biology of Intracellular Phase Transitions

Annual Review of Biophysics, Год журнала: 2020, Номер 49(1), С. 107 - 133

Опубликована: Янв. 31, 2020

Many biomolecular condensates appear to form via spontaneous or driven processes that have the hallmarks of intracellular phase transitions. This suggests a common underlying physical framework might govern formation functionally and compositionally unrelated condensates. In this review, we summarize recent work leverages stickers-and-spacers adapted from field associative polymers for understanding how multivalent protein RNA molecules drive transitions give rise We discuss valence stickers impacts driving forces condensate elaborate on can be distinguished spacers in different contexts. touch impact sticker- spacer-mediated interactions rheological properties show model mapped known drivers types

Язык: Английский

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Liquid–Liquid Phase Separation in Disease

Annual Review of Genetics, Год журнала: 2019, Номер 53(1), С. 171 - 194

Опубликована: Авг. 20, 2019

We have made rapid progress in recent years identifying the genetic causes of many human diseases. However, despite this progress, our mechanistic understanding these diseases is often incomplete. This a problem because it limits ability to develop effective disease treatments. To overcome limitation, we need new concepts describe and comprehend complex mechanisms underlying Condensate formation by phase separation emerges as principle explain organization living cells. In review, present emerging evidence that aberrant forms condensates are associated with diseases, including cancer, neurodegeneration, infectious examine driven condensates, point out opportunities for therapeutic interventions. conclude provides useful framework understand fight some most severe

Язык: Английский

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The molecular language of membraneless organelles

Journal of Biological Chemistry, Год журнала: 2018, Номер 294(18), С. 7115 - 7127

Опубликована: Июль 25, 2018

Язык: Английский

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A framework for understanding the functions of biomolecular condensates across scales

Nature Reviews Molecular Cell Biology, Год журнала: 2020, Номер 22(3), С. 215 - 235

Опубликована: Ноя. 9, 2020

Язык: Английский

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Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome

Cell, Год журнала: 2018, Номер 175(6), С. 1481 - 1491.e13

Опубликована: Ноя. 1, 2018

Язык: Английский

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