Transcription Factors Activate Genes through the Phase-Separation Capacity of Their Activation Domains

Cell, Journal Year: 2018, Volume and Issue: 175(7), P. 1842 - 1855.e16

Published: Nov. 15, 2018

Language: Английский

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Mediator and RNA polymerase II clusters associate in transcription-dependent condensates

Science, Journal Year: 2018, Volume and Issue: 361(6400), P. 412 - 415

Published: June 21, 2018

Models of gene control have emerged from genetic and biochemical studies, with limited consideration the spatial organization dynamics key components in living cells. We used live-cell superresolution light-sheet imaging to study Mediator coactivator RNA polymerase II (Pol II) directly. Pol each form small transient large stable clusters embryonic stem are colocalized clusters, which associate chromatin, properties phase-separated condensates, sensitive transcriptional inhibitors. suggest that Mediator, recruited by transcription factors at or clustered enhancer elements, interact condensates vivo.

Language: Английский

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Long non-coding RNAs: definitions, functions, challenges and recommendations

Nature Reviews Molecular Cell Biology, Journal Year: 2023, Volume and Issue: 24(6), P. 430 - 447

Published: Jan. 3, 2023

Language: Английский

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Long-range enhancer–promoter contacts in gene expression control

Nature Reviews Genetics, Journal Year: 2019, Volume and Issue: 20(8), P. 437 - 455

Published: May 13, 2019

Language: Английский

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Organization of Chromatin by Intrinsic and Regulated Phase Separation

Cell, Journal Year: 2019, Volume and Issue: 179(2), P. 470 - 484.e21

Published: Sept. 19, 2019

Language: Английский

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Physical Principles Underlying the Complex Biology of Intracellular Phase Transitions

Annual Review of Biophysics, Journal Year: 2020, Volume and Issue: 49(1), P. 107 - 133

Published: Jan. 31, 2020

Many biomolecular condensates appear to form via spontaneous or driven processes that have the hallmarks of intracellular phase transitions. This suggests a common underlying physical framework might govern formation functionally and compositionally unrelated condensates. In this review, we summarize recent work leverages stickers-and-spacers adapted from field associative polymers for understanding how multivalent protein RNA molecules drive transitions give rise We discuss valence stickers impacts driving forces condensate elaborate on can be distinguished spacers in different contexts. touch impact sticker- spacer-mediated interactions rheological properties show model mapped known drivers types

Language: Английский

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Liquid–Liquid Phase Separation in Disease

Annual Review of Genetics, Journal Year: 2019, Volume and Issue: 53(1), P. 171 - 194

Published: Aug. 20, 2019

We have made rapid progress in recent years identifying the genetic causes of many human diseases. However, despite this progress, our mechanistic understanding these diseases is often incomplete. This a problem because it limits ability to develop effective disease treatments. To overcome limitation, we need new concepts describe and comprehend complex mechanisms underlying Condensate formation by phase separation emerges as principle explain organization living cells. In review, present emerging evidence that aberrant forms condensates are associated with diseases, including cancer, neurodegeneration, infectious examine driven condensates, point out opportunities for therapeutic interventions. conclude provides useful framework understand fight some most severe

Language: Английский

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The molecular language of membraneless organelles

Journal of Biological Chemistry, Journal Year: 2018, Volume and Issue: 294(18), P. 7115 - 7127

Published: July 25, 2018

Language: Английский

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A framework for understanding the functions of biomolecular condensates across scales

Nature Reviews Molecular Cell Biology, Journal Year: 2020, Volume and Issue: 22(3), P. 215 - 235

Published: Nov. 9, 2020

Language: Английский

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Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome

Cell, Journal Year: 2018, Volume and Issue: 175(6), P. 1481 - 1491.e13

Published: Nov. 1, 2018

Language: Английский

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