A functional selection reveals previously undetected anti-phage defence systems in the E. coli pangenome DOI Creative Commons
Christopher N. Vassallo, Christopher R. Doering, Megan L. Littlehale

и другие.

Nature Microbiology, Год журнала: 2022, Номер 7(10), С. 1568 - 1579

Опубликована: Сен. 19, 2022

The ancient, ongoing coevolutionary battle between bacteria and their viruses, bacteriophages, has given rise to sophisticated immune systems including restriction-modification CRISPR-Cas. Many additional anti-phage have been identified using computational approaches based on genomic co-location within defence islands, but these screens may not be exhaustive. Here we developed an experimental selection scheme agnostic context identify in 71 diverse E. coli strains. Our results unveil 21 conserved systems, none of which were previously detected as enriched islands. Additionally, our work indicates that intact prophages mobile genetic elements are primary reservoirs distributors coli, with typically carried specific locations or hotspots. These hotspots encode dozens uncharacterized system candidates. findings reveal extended landscape antiviral immunity provide approach for mapping other species.

Язык: Английский

Phage Therapy in the Postantibiotic Era DOI Open Access
Fernando Gordillo Altamirano, Jeremy J. Barr

Clinical Microbiology Reviews, Год журнала: 2019, Номер 32(2)

Опубликована: Янв. 15, 2019

Antibiotic resistance is arguably the biggest current threat to global health. An increasing number of infections are becoming harder or almost impossible treat, carrying high morbidity, mortality, and financial cost. The therapeutic use bacteriophages, viruses that infect kill bacteria, well suited be part multidimensional strategies combat antibiotic resistance. Although phage therapy was first implemented a century ago, it brought standstill after successful introduction antibiotics. Now, with rise resistance, experiencing well-deserved rebirth. Among admittedly vast literature recently published on this topic, review aims provide forward-looking perspective its role in modern society. We cover key points crisis then explain biological evolutionary principles support phages, their interaction immune system, comparison therapy. By going through up-to-date reports and, whenever possible, human clinical trials, we examine versatility discuss conventional approaches as novel strategies, including phage-antibiotic combinations, phage-derived enzymes, exploitation mechanisms, bioengineering. Finally, benefits beyond perspective, opportunities for scientific outreach effective education, interdisciplinary collaboration, cultural economic growth, even innovative social media, making case more than just an alternative

Язык: Английский

Процитировано

777

The arms race between bacteria and their phage foes DOI

Hannah G. Hampton,

Bridget N. J. Watson, Peter C. Fineran

и другие.

Nature, Год журнала: 2020, Номер 577(7790), С. 327 - 336

Опубликована: Янв. 15, 2020

Язык: Английский

Процитировано

717

The pan-immune system of bacteria: antiviral defence as a community resource DOI
Aude Bernheim, Rotem Sorek

Nature Reviews Microbiology, Год журнала: 2019, Номер 18(2), С. 113 - 119

Опубликована: Ноя. 6, 2019

Язык: Английский

Процитировано

548

Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration DOI
Sanne E. Klompe, Phuc Leo H. Vo, Tyler S. Halpin-Healy

и другие.

Nature, Год журнала: 2019, Номер 571(7764), С. 219 - 225

Опубликована: Июнь 12, 2019

Язык: Английский

Процитировано

537

The evolution of antibiotic resistance DOI
R. Craig MacLean, Álvaro San Millán

Science, Год журнала: 2019, Номер 365(6458), С. 1082 - 1083

Опубликована: Сен. 12, 2019

Clinically relevant evolution studies are needed to help fight the spread of antibiotic resistance

Язык: Английский

Процитировано

503

Cyclic GMP–AMP signalling protects bacteria against viral infection DOI
Daniel Cohen, Sarah Melamed, Adi Millman

и другие.

Nature, Год журнала: 2019, Номер 574(7780), С. 691 - 695

Опубликована: Сен. 18, 2019

Язык: Английский

Процитировано

478

Diverse enzymatic activities mediate antiviral immunity in prokaryotes DOI
Linyi Gao, Han Altae-Tran,

Francisca Böhning

и другие.

Science, Год журнала: 2020, Номер 369(6507), С. 1077 - 1084

Опубликована: Авг. 28, 2020

Bacteria and archaea are frequently attacked by viruses other mobile genetic elements rely on dedicated antiviral defense systems, such as restriction endonucleases CRISPR, to survive. The enormous diversity of suggests that more types systems exist than currently known. By systematic gene prediction heterologous reconstitution, here we discover 29 widespread cassettes, collectively present in 32% all sequenced bacterial archaeal genomes, mediate protection against specific bacteriophages. These incorporate enzymatic activities not previously implicated defense, including RNA editing retron satellite DNA synthesis. In addition, computationally predict a diverse set putative genes remain be characterized. results highlight an immense array molecular functions microbes use viruses.

Язык: Английский

Процитировано

468

Systematic and quantitative view of the antiviral arsenal of prokaryotes DOI Creative Commons
Florian Tesson, Hervé Alexandre, Ernest Mordret

и другие.

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Май 10, 2022

Bacteria and archaea have developed multiple antiviral mechanisms, genomic evidence indicates that several of these systems co-occur in the same strain. Here, we introduce DefenseFinder, a tool automatically detects known prokaryotic genomes. We use DefenseFinder to analyse 21000 fully sequenced genomes, find strategies vary drastically between phyla, species strains. Variations composition correlate with genome size, viral threat, lifestyle traits. will facilitate large-scale analysis defense study host-virus interactions prokaryotes.

Язык: Английский

Процитировано

457

Functionally diverse type V CRISPR-Cas systems DOI Open Access

Winston X. Yan,

Pratyusha Hunnewell, Lauren E. Alfonse

и другие.

Science, Год журнала: 2018, Номер 363(6422), С. 88 - 91

Опубликована: Дек. 7, 2018

Additional, diverse CRISPR systems have been revolutionizing molecular biology. Mining the metagenomic database, Yan et al. systematically discovered additional subtypes of type V CRISPR-Cas systems. The Cas12 effectors displayed a range activities, including target and collateral cleavage single-stranded RNA DNA, as well double-stranded DNA nicking cleavage. These nuclease activities suggest how an ancient transposase may evolved into various expand nucleic acid detection genome-editing toolbox. Science , this issue p. 88

Язык: Английский

Процитировано

453

Interactions between bacterial and phage communities in natural environments DOI
Anne Chevallereau, Benoît J. Pons, Stineke van Houte

и другие.

Nature Reviews Microbiology, Год журнала: 2021, Номер 20(1), С. 49 - 62

Опубликована: Авг. 9, 2021

Язык: Английский

Процитировано

435