Tissue clearing and its applications in neuroscience

Nature reviews. Neuroscience, Год журнала: 2020, Номер 21(2), С. 61 - 79

Опубликована: Янв. 2, 2020

Язык: Английский

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The connectome of the adult Drosophila mushroom body provides insights into function

eLife, Год журнала: 2020, Номер 9

Опубликована: Дек. 14, 2020

Making inferences about the computations performed by neuronal circuits from synapse-level connectivity maps is an emerging opportunity in neuroscience. The mushroom body (MB) well positioned for developing and testing such approach due to its conserved architecture, recently completed dense connectome, extensive prior experimental studies of roles learning, memory, activity regulation. Here, we identify new components MB circuit Drosophila, including visual input output neurons (MBONs) with direct connections descending neurons. We find unexpected structure sensory inputs, transfer information different modalities MBONs, modulation that dopaminergic (DANs). provide insights into circuitry used integrate outputs, between central complex inputs DANs, feedback MBONs. Our results a foundation further theoretical work.

Язык: Английский

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BigStitcher: reconstructing high-resolution image datasets of cleared and expanded samples

Nature Methods, Год журнала: 2019, Номер 16(9), С. 870 - 874

Опубликована: Авг. 5, 2019

Язык: Английский

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Spatial and temporal scales of dopamine transmission

Nature reviews. Neuroscience, Год журнала: 2021, Номер 22(6), С. 345 - 358

Опубликована: Апрель 9, 2021

Язык: Английский

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A hybrid erbium(III)–bacteriochlorin near-infrared probe for multiplexed biomedical imaging

Nature Materials, Год журнала: 2021, Номер 20(11), С. 1571 - 1578

Опубликована: Июль 29, 2021

Язык: Английский

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Light sheet fluorescence microscopy

Nature Reviews Methods Primers, Год журнала: 2021, Номер 1(1)

Опубликована: Ноя. 3, 2021

Язык: Английский

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The Functional Impact of Mitochondrial Structure Across Subcellular Scales

Frontiers in Physiology, Год журнала: 2020, Номер 11

Опубликована: Ноя. 11, 2020

Mitochondria are key determinants of cellular health. However, the functional role mitochondria varies from cell to depending on relative demands for energy distribution, metabolite biosynthesis, and/or signaling. In order support specific needs different types, mitochondrial capacity can be optimized in part by modulating structure across several spatial scales. Here we discuss implications altering with an emphasis physiological trade-offs associated configurations. Within a mitochondrion, increasing amount cristae inner membrane improves conversion and free radical-mediated signaling but may come at expense matrix space where enzymes critical biosynthesis reside. Electrically isolating individual could provide protective mechanism limit spread dysfunction within mitochondrion also slow response time increase demand. For mitochondria, those relatively greater surface areas facilitate interactions cytosol or other organelles more costly remove through mitophagy due need larger phagophore membranes. At network scale, large, stable reticulum structural pathway distribution communication long distances yet enable rapid spreading localized dysfunction. Highly dynamic networks allow frequent content mixing require constant remodeling accommodate movement mitochondria. The formation contact sites between provides specialized direct transfer organelles. number any given organelle reduces area available as well exchange cytosol. Though precise mechanisms guiding coordinated multi-scale configurations observed types have elucidated, it is clear that tailored every level optimize function meet demands.

Язык: Английский

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Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution

Nature Methods, Год журнала: 2019, Номер 16(11), С. 1109 - 1113

Опубликована: Окт. 31, 2019

Язык: Английский

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Light-Sheet Microscopy in Neuroscience

Annual Review of Neuroscience, Год журнала: 2019, Номер 42(1), С. 295 - 313

Опубликована: Июль 8, 2019

Light-sheet microscopy is an imaging approach that offers unique advantages for a diverse range of neuroscience applications. Unlike point-scanning techniques such as confocal and two-photon microscopy, light-sheet microscopes illuminate entire plane tissue, while this onto camera. Although early implementations light sheet were optimized longitudinal embryonic development in small specimens, emerging are capable capturing images freely moving, unconstrained specimens even the intact vivo mammalian brain. Meanwhile, photobleaching signal-to-noise benefits afforded by microscopy's parallelized detection deliver ability to perform volumetric at much higher speeds than can be achieved using point scanning. This review describes basic principles evolution followed perspectives on applications opportunities both large, cleared, expanded neural tissues high-speed, functional vivo.

Язык: Английский

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An atlas of nano-enabled neural interfaces

Nature Nanotechnology, Год журнала: 2019, Номер 14(7), С. 645 - 657

Опубликована: Июль 1, 2019

Язык: Английский

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