Advances in research on immunocyte iron metabolism, ferroptosis, and their regulatory roles in autoimmune and autoinflammatory diseases DOI Creative Commons

Liuting Zeng,

Kailin Yang,

Ganpeng Yu

и другие.

Cell Death and Disease, Год журнала: 2024, Номер 15(7)

Опубликована: Июль 4, 2024

Abstract Autoimmune diseases commonly affect various systems, but their etiology and pathogenesis remain unclear. Currently, increasing research has highlighted the role of ferroptosis in immune regulation, with cells being a crucial component body’s system. This review provides an overview discusses relationship between ferroptosis, programmed cell death cells, autoimmune diseases. Additionally, it summarizes key targets such as GPX4 TFR, responses. Furthermore, release multiple molecules, including damage-associated molecular patterns (DAMPs), following by is examined, these molecules further influence differentiation function thereby affecting occurrence progression Moreover, secrete factors or metabolites, which also impact target organs tissues involved Iron chelators, chloroquine its derivatives, antioxidants, calreticulin have been demonstrated to be effective animal studies for certain diseases, exerting anti-inflammatory immunomodulatory effects. Finally, brief summary future perspectives on are provided, aiming guide disease treatment strategies.

Язык: Английский

Abrine, an IDO1 inhibitor, suppresses the immune escape and enhances the immunotherapy of anti-PD-1 antibody in hepatocellular carcinoma DOI Creative Commons
Xiaowei Liang, Hongwei Gao, Jian Xiao

и другие.

Frontiers in Immunology, Год журнала: 2023, Номер 14

Опубликована: Май 24, 2023

Background Indoleamine-2,3-dioxygenase 1 (IDO1) is responsible for tumor immune escape by regulating T cell-associated responses and promoting the activation of immunosuppressive. Given vital role IDO1 in response, further investigation on regulation tumors needed. Methods Herein, we used ELISA kit to detect interferon-gamma (IFN-γ), Tryptophan (Trp), kynurenic acid (Kyn) levels; western blot, Flow cytometry, immunofluorescence assays detected expression proteins; Molecular docking assay, SPR assay Cellular Thermal Shift Assay (CETSA) were interaction between Abrine; nano live label-free system was phagocytosis activity; xenografts animal experiments explore anti-tumor effect flow cytometry cells changes. Results The important inflammatory response cytokine (IFN-γ) up-regulated cancer through methylation 6-methyladenosine (m6A) m6A modification RNA, metabolism Trp into Kyn, JAK1/STAT1 signaling pathway, which could be inhibited inhibitor Abrine. CD47 IFN-γ-stimulated genes (ISGs) prevents macrophages, leading escape, this Abrine both vivo vitro. PD-1/PD-L1 axis an checkpoint overexpression PD-1 or PD-L1 promotes suppression, while study inhibit tissue. combination treatment anti-PD-1 antibody has a synergistic suppressing growth up-regulating CD4 + CD8 cells, down-regulating Foxp3 Treg inhibiting IDO1, CD47, PD-L1. Conclusion Overall, reveals that as inhibition with HCC.

Язык: Английский

Процитировано

25

The evolutionary theory of cancer: challenges and potential solutions DOI
Lucie Laplane, Carlo C. Maley

Nature reviews. Cancer, Год журнала: 2024, Номер 24(10), С. 718 - 733

Опубликована: Сен. 10, 2024

Язык: Английский

Процитировано

15

Single-cell multiomics reveals the interplay of clonal evolution and cellular plasticity in hepatoblastoma DOI Creative Commons
Amélie Roehrig, Théo Z. Hirsch, Aurore Pire

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Апрель 8, 2024

Abstract Hepatoblastomas (HB) display heterogeneous cellular phenotypes that influence the clinical outcome, but underlying mechanisms are poorly understood. Here, we use a single-cell multiomic strategy to unravel molecular determinants of this plasticity. We identify continuum HB cell states between hepatocytic (scH), liver progenitor (scLP) and mesenchymal (scM) differentiation poles, with an intermediate scH/LP population bordering scLP scH areas in spatial transcriptomics. Chromatin accessibility landscapes reveal gene regulatory networks each pole, sequence transcription factor activations state transitions. Single-cell mapping somatic alterations reveals clonal architecture tumor, showing genetic subclone displays its own range plasticity across states. The most subclones, overexpressing stem DNA repair genes, proliferate faster after neo-adjuvant chemotherapy. These results highlight how interplay evolution epigenetic shapes potential subclones respond

Язык: Английский

Процитировано

14

Unveiling Alterations of Epigenetic Modifications and Chromatin Architecture Leading to Lipid Metabolic Reprogramming during the Evolutionary Trastuzumab Adaptation of HER2‐Positive Breast Cancer DOI Creative Commons
Ningjun Duan, Yijia Hua, Xueqi Yan

и другие.

Advanced Science, Год журнала: 2024, Номер 11(18)

Опубликована: Март 9, 2024

Abstract Secondary trastuzumab resistance represents an evolutionary adaptation of HER2‐positive breast cancer during anti‐HER2 treatment. Most current studies have tended to prioritize HER2 and its associated signaling pathways, often overlooking broader but seemingly less relevant cellular processes, along with their genetic epigenetic mechanisms. Here, transcriptome data is not only characterized also examined epigenomic 3D genome architecture information in both trastuzumab‐sensitive secondary‐resistant cells. The findings reveal that the global metabolic reprogramming may stem from genome‐wide alterations histone modifications chromatin structure. Specifically, transcriptional activities key genes involved lipid metabolism appear be regulated by variant promoter H3K27me3 H3K4me3 modifications, as well promoter‐enhancer interactions. These discoveries offer valuable insights into how cells adapt anti‐tumor drugs potential impact future diagnostic treatment strategies.

Язык: Английский

Процитировано

13

Enhancing cancer therapy: the integration of oncolytic virus therapy with diverse treatments DOI Creative Commons
Yan Zhuo, Zhengbo Zhang, Yanan Chen

и другие.

Cancer Cell International, Год журнала: 2024, Номер 24(1)

Опубликована: Июль 11, 2024

Abstract As one of the significant challenges to human health, cancer has long been a focal point in medical treatment. With ongoing advancements field medicine, numerous methodologies for therapy have emerged, among which oncolytic virus gained considerable attention. However, viruses still exhibit limitations. Combining them with various therapies can further enhance efficacy treatment, offering renewed hope patients. In recent research, scientists recognized promising prospect amalgamating diverse treatments, potentially surmounting restrictions singular approaches. The central concept this combined revolves around leveraging incite localized tumor inflammation, augmenting immune response immunotherapeutic efficacy. Through approach, patient's system better recognize and eliminate cells, simultaneously reducing evasion mechanisms against system. This review delves deeply into latest research progress concerning integration treatments its role types therapy. We aim analyze mechanisms, advantages, potential challenges, future directions combination By extensively exploring field, we instill fight cancer.

Язык: Английский

Процитировано

13

Genetic and epigenetic instability as an underlying driver of progression and aggressive behavior in IDH-mutant astrocytoma DOI Creative Commons
Timothy E. Richardson, Jamie M. Walker, Dolores Hambardzumyan

и другие.

Acta Neuropathologica, Год журнала: 2024, Номер 148(1)

Опубликована: Июль 16, 2024

Abstract In recent years, the classification of adult-type diffuse gliomas has undergone a revolution, wherein specific molecular features now represent defining diagnostic criteria IDH-wild-type glioblastomas, IDH-mutant astrocytomas, and 1p/19q-codeleted oligodendrogliomas. With introduction 2021 WHO CNS classification, additional alterations are integrated into grading these tumors, given equal weight to traditional histologic features. However, there remains great deal heterogeneity in patient outcome even within established tumor subclassifications that is unexplained by currently codified alterations, particularly astrocytoma category. There also significant intercellular genetic epigenetic plasticity with resulting phenotypic heterogeneity, making tumors remarkably adaptable robust, presenting barrier design effective therapeutics. Herein, we review mechanisms consequences instability, including chromosomal instability (CIN), microsatellite (MSI)/mismatch repair (MMR) deficits, underlying biology, tumorigenesis, progression astrocytomas. We discuss contribution high-resolution transcriptomics studies toward single-cell resolution. While intratumoral well-known feature gliomas, various processes only recently been considered as potential driver aggressiveness. CIN an independent, adverse effect on survival, similar grade homozygous CDKN2A deletion, while MMR mutation associated poor overall survival univariate analysis but highly correlated higher histologic/molecular other aggressive These forms genomic which may significantly affect natural response therapy, ultimately clinical for patients, potentially measurable could aid diagnosis, grading, prognosis, development personalized

Язык: Английский

Процитировано

13

Whole-Genome Mapping of Epigenetic Modification of 5-Formylcytosine at Single-Base Resolution by Chemical Labeling Enrichment and Deamination Sequencing DOI

Jiang-Hui Ding,

Gaojie Li, Jun Xiong

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 96(11), С. 4726 - 4735

Опубликована: Март 7, 2024

DNA cytosine methylation (5-methylcytosine, 5mC) is a predominant epigenetic modification that plays critical role in variety of biological and pathological processes mammals. In active demethylation, the 10-11 translocation (TET) dioxygenases can sequentially oxidize 5mC to generate three modified forms cytosine, 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), 5-carboxylcytosine (5caC). Beyond being demethylation intermediate, recent studies have shown 5fC has regulatory functions gene expression chromatin organization. While some methods been developed detect 5fC, genome-wide mapping at base resolution still highly desirable. Herein, we propose chemical labeling enrichment deamination sequencing (CLED-seq) method for detecting genomic single-base resolution. The CLED-seq utilizes selective 5fC-containing fragments, followed by mediated apolipoprotein B mRNA-editing catalytic polypeptide-like 3A (APOBEC3A or A3A) sequencing. process, while all C, 5mC, 5hmC are interpreted as T during sequencing, read enabling precise detection DNA. Using proposed method, accomplished mouse embryonic stem cells. study revealed promoter regions enriched with overlapped H3K4me1, H3K4me3, H3K27ac marks. These findings suggest correlation between marks mESCs. conclusion, straightforward, bisulfite-free offers valuable tool genomes

Язык: Английский

Процитировано

12

Simultaneous detection of 5-methylcytosine and 5-hydroxymethylcytosine at specific genomic loci by engineered deaminase-assisted sequencing DOI Creative Commons

Neng‐Bin Xie,

Min Wang,

Tong‐Tong Ji

и другие.

Chemical Science, Год журнала: 2024, Номер 15(26), С. 10073 - 10083

Опубликована: Янв. 1, 2024

We developed the EDA-seq method, which enables simultaneous and quantitative detection of C, 5mC, 5hmC in DNA at single-base resolution.

Язык: Английский

Процитировано

12

Epigenetic modifications in obesity‐associated diseases DOI Creative Commons

Yiqian Long,

Chao Mao, Shuang Liu

и другие.

MedComm, Год журнала: 2024, Номер 5(2)

Опубликована: Фев. 1, 2024

The global prevalence of obesity has reached epidemic levels, significantly elevating the susceptibility to various cardiometabolic conditions and certain types cancer. In addition causing metabolic abnormalities such as insulin resistance (IR), elevated blood glucose lipids, ectopic fat deposition, can also damage pancreatic islet cells, endothelial cardiomyocytes through chronic inflammation, even promote development a microenvironment conducive cancer initiation. Improper dietary habits lack physical exercise are important behavioral factors that increase risk obesity, which affect gene expression epigenetic modifications. Epigenetic alterations occur in early stage some reversible, while others persist over time lead obesity-related complications. Therefore, dynamic adjustability modifications be leveraged reverse obesity-associated diseases interventions, drugs, bariatric surgery. This review provides comprehensive summary impact regulation on initiation cancers, type 2 diabetes, cardiovascular diseases, establishing theoretical basis for prevention, diagnosis, treatment these conditions.

Язык: Английский

Процитировано

11

Small-molecule-based targeted therapy in liver cancer DOI
Ming Yue, Yanqiu Gong,

Xuewen Fu

и другие.

Molecular Therapy, Год журнала: 2024, Номер 32(10), С. 3260 - 3287

Опубликована: Авг. 8, 2024

Язык: Английский

Процитировано

11