All models are wrong, but some are useful: Establishing standards for stem cell-based embryo models DOI Creative Commons
Eszter Pósfai, Fredrik Lanner, Carla Mulas

и другие.

Stem Cell Reports, Год журнала: 2021, Номер 16(5), С. 1117 - 1141

Опубликована: Май 1, 2021

Detailed studies of the embryo allow an increasingly mechanistic understanding development, which has proved profound relevance to human disease. The last decade seen in vitro cultured stem cell-based models development flourish, provide alternative for accessible experimentation. However, usefulness any model will be determined by how accurately it reflects vivo embryonic and/or extent facilitates new discoveries. Stringent benchmarking is thus important consideration this growing field. Here we overview means evaluate both properties cells, building blocks most models, as well current and future models.

Язык: Английский

Harnessing the power of artificial intelligence for human living organoid research DOI Creative Commons
Hui Wang, Xiangyang Li, Xiaoyan You

и другие.

Bioactive Materials, Год журнала: 2024, Номер 42, С. 140 - 164

Опубликована: Авг. 30, 2024

As a powerful paradigm, artificial intelligence (AI) is rapidly impacting every aspect of our day-to-day life and scientific research through interdisciplinary transformations. Living human organoids (LOs) have great potential for

Язык: Английский

Процитировано

10
Mouse embryo geometry drives formation of robust signaling gradients through receptor localization DOI Creative Commons
Zhechun Zhang, Steven Zwick, Ethan B Loew

и другие.

Nature Communications, Год журнала: 2019, Номер 10(1)

Опубликована: Окт. 4, 2019

Abstract Morphogen signals are essential for cell fate specification during embryogenesis. Some receptors that sense these morphogens known to localize only the apical or basolateral membrane of polarized lines in vitro. How such localization affects morphogen sensing and patterning developing embryo remains unknown. Here, we show formation a robust BMP signaling gradient early mouse depends on restricted, receptors. The mis-localization results ectopic epiblast vivo. With evidence from mathematical modeling, human embryonic stem cells vitro, embryos vivo, find geometric compartmentalization ligands creates is buffered against fluctuations. Our demonstrate importance receptor geometry shaping

Язык: Английский

Процитировано

76
National Human Embryo and Embryoid Research Policies: A Survey of 22 Top Research-Intensive Countries DOI Creative Commons
Kirstin R.W. Matthews,

Daniel Moralí

Regenerative Medicine, Год журнала: 2020, Номер 15(7), С. 1905 - 1917

Опубликована: Июль 1, 2020

Research using human embryos and embryoids has expanded in recent years due to technological advances. Surveying laws guidelines among the top research development (R&D) investing nations highlights existing barriers expanding this area of research. Of 22 surveyed, we found 12 countries with a 14-day limit, one seven-day five prohibitions four without national or that limit prohibit embryo Sixteen define an related entities, limiting embryoid Other are ambiguous relation research, leave unanswered questions regarding what is permitted restricted need additional clarity for researchers.

Язык: Английский

Процитировано

70
Embryo implantation in the laboratory: an update on current techniques DOI
Samuel Ojosnegros, Anna Seriola, Amélie Luise Godeau

и другие.

Human Reproduction Update, Год журнала: 2020, Номер 27(3), С. 501 - 530

Опубликована: Ноя. 5, 2020

Abstract BACKGROUND The embryo implantation process is crucial for the correct establishment and progress of pregnancy. During implantation, blastocyst trophectoderm cells attach to epithelium endometrium, triggering intense cell-to-cell crosstalk that leads trophoblast outgrowth, invasion endometrial tissue, formation placenta. However, this process, which vital foetal development in utero, still elusive experimentation because its inaccessibility. Experimental cumbersome impractical adult animal models inconceivable humans. OBJECTIVE AND RATIONALE A number custom experimental solutions have been proposed recreate different stages vitro, by combining a human (or surrogate) surrogate tissue). In vitro allow rapid high-throughput interrogation embryos cells, efficient screening molecules, such as cytokines, drugs, or transcription factors, control receptivity endometrium. broad selection available systems makes it complicated decide system best fits needs specific experiment scientific question. To orient reader, review will explore options literature, classify them into amenable categories based on embryo/cell pairs employed. goal give an overview tools study complex explain differences between them, including advantages disadvantages each system. SEARCH METHODS We performed comprehensive literature come up with mimic ranging from initial apposition later gastrulation. also recent breakthrough advances stem organoids, assembling embryo-like structures tissues. OUTCOMES highlight most relevant describe significant experiments. focus contributed reproduction discovering molecules hormones, signalling factors cytokines. WIDER IMPLICATIONS momentum field growing thanks use build tissues, bioengineering extend life culture. propose merge methods derived fields develop new covering wider window process.

Язык: Английский

Процитировано

67
All models are wrong, but some are useful: Establishing standards for stem cell-based embryo models DOI Creative Commons
Eszter Pósfai, Fredrik Lanner, Carla Mulas

и другие.

Stem Cell Reports, Год журнала: 2021, Номер 16(5), С. 1117 - 1141

Опубликована: Май 1, 2021

Detailed studies of the embryo allow an increasingly mechanistic understanding development, which has proved profound relevance to human disease. The last decade seen in vitro cultured stem cell-based models development flourish, provide alternative for accessible experimentation. However, usefulness any model will be determined by how accurately it reflects vivo embryonic and/or extent facilitates new discoveries. Stringent benchmarking is thus important consideration this growing field. Here we overview means evaluate both properties cells, building blocks most models, as well current and future models.

Язык: Английский

Процитировано

54