Nature Communications,
Год журнала:
2021,
Номер
12(1)
Опубликована: Июнь 7, 2021
Plants
utilise
intracellular
nucleotide-binding,
leucine-rich
repeat
(NLR)
immune
receptors
to
detect
pathogen
effectors
and
activate
local
systemic
defence.
NRG1
ADR1
"helper"
NLRs
(RNLs)
cooperate
with
enhanced
disease
susceptibility
1
(EDS1),
senescence-associated
gene
101
(SAG101)
phytoalexin-deficient
4
(PAD4)
lipase-like
proteins
mediate
signalling
from
TIR
domain
NLR
(TNLs).
The
mechanism
of
RNL/EDS1
family
protein
cooperation
is
not
understood.
Here,
we
present
genetic
molecular
evidence
for
exclusive
EDS1/SAG101/NRG1
EDS1/PAD4/ADR1
co-functions
in
TNL
immunity.
Using
immunoprecipitation
mass
spectrometry,
show
effector
recognition-dependent
interaction
EDS1
SAG101,
but
PAD4.
An
EDS1-SAG101
complex
interacts
NRG1,
EDS1-PAD4
ADR1,
an
immune-activated
state.
requires
intact
nucleotide-binding
P-loop
motif,
a
functional
EP
its
partner
induced
association
Thus,
two
distinct
modules
(NRG1/EDS1/SAG101
ADR1/EDS1/PAD4)
receptor
defence
signalling.
The Plant Cell,
Год журнала:
2022,
Номер
34(5), С. 1447 - 1478
Опубликована: Фев. 10, 2022
Understanding
the
plant
immune
system
is
crucial
for
using
genetics
to
protect
crops
from
diseases.
Plants
resist
pathogens
via
a
two-tiered
innate
detection-and-response
system.
The
first
Resistance
(R)
gene
was
cloned
in
1992
.
Since
then,
many
cell-surface
pattern
recognition
receptors
(PRRs)
have
been
identified,
and
R
genes
that
encode
intracellular
nucleotide-binding
leucine-rich
repeat
(NLRs)
cloned.
Here,
we
provide
list
of
characterized
PRRs
NLRs.
In
addition
receptors,
components
signaling
networks
were
discovered
over
last
30
years.
We
review
pathways,
physiological
responses,
molecular
regulation
both
PRR-
NLR-mediated
immunity.
Recent
studies
reinforced
importance
interactions
between
two
systems.
an
overview
immunity,
highlighting
challenges
perspectives
future
research.
Science,
Год журнала:
2020,
Номер
369(6507), С. 1077 - 1084
Опубликована: Авг. 28, 2020
Bacteria
and
archaea
are
frequently
attacked
by
viruses
other
mobile
genetic
elements
rely
on
dedicated
antiviral
defense
systems,
such
as
restriction
endonucleases
CRISPR,
to
survive.
The
enormous
diversity
of
suggests
that
more
types
systems
exist
than
currently
known.
By
systematic
gene
prediction
heterologous
reconstitution,
here
we
discover
29
widespread
cassettes,
collectively
present
in
32%
all
sequenced
bacterial
archaeal
genomes,
mediate
protection
against
specific
bacteriophages.
These
incorporate
enzymatic
activities
not
previously
implicated
defense,
including
RNA
editing
retron
satellite
DNA
synthesis.
In
addition,
computationally
predict
a
diverse
set
putative
genes
remain
be
characterized.
results
highlight
an
immense
array
molecular
functions
microbes
use
viruses.
Tetrameric
immune
receptors
Nucleotide-binding/leucine-rich
repeat
(NLR)
detect
pathogen
effectors
and
trigger
a
plant's
response.
Two
groups
have
now
defined
the
structures
of
two
NLRs
that
carry
Toll-like
interleukin-1
receptor
(TIR)
domains
(TIR-NLRs)
(see
Perspective
by
Tian
Li).
Ma
et
al.
studied
Arabidopsis
thaliana
TIR-NLR
RPP1
(recognition
Peronospora
parasitica
1)
its
response
to
from
an
oomycete
pathogen.
Martin
Nicotiana
benthamiana
ROQ1
XopQ
Xanthomonas
effector.
Both
found
these
TIR-NLRs
formed
tetramers
that,
when
activated
binding
effector,
exposed
active
site
nicotinamide
adenine
dinucleoside
(NAD)
hydrolase.
Thus,
recognition
effector
initiates
NAD
hydrolysis
begins
Science
,
this
issue
p.
eabe3069
eabd9993
;
see
also
1163
Tetrameric
immune
receptors
Nucleotide-binding/leucine-rich
repeat
(NLR)
detect
pathogen
effectors
and
trigger
a
plant's
response.
Two
groups
have
now
defined
the
structures
of
two
NLRs
that
carry
Toll-like
interleukin-1
receptor
(TIR)
domains
(TIR-NLRs)
(see
Perspective
by
Tian
Li).
Ma
et
al.
studied
Arabidopsis
thaliana
TIR-NLR
RPP1
(recognition
Peronospora
parasitica
1)
its
response
to
from
an
oomycete
pathogen.
Martin
Nicotiana
benthamiana
ROQ1
XopQ
Xanthomonas
effector.
Both
found
these
TIR-NLRs
formed
tetramers
that,
when
activated
binding
effector,
exposed
active
site
nicotinamide
adenine
dinucleoside
(NAD)
hydrolase.
Thus,
recognition
effector
initiates
NAD
hydrolysis
begins
Science
,
this
issue
p.
eabe3069
eabd9993
;
see
also
1163
