Astrocyte-Neuron Signaling in Synaptogenesis DOI Creative Commons

Lili Shan,

Tongran Zhang,

Kevin Fan

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2021, Номер 9

Опубликована: Июль 2, 2021

Astrocytes are the key component of central nervous system (CNS), serving as pivotal regulators neuronal synapse formation and maturation through their ability to dynamically bidirectionally communicate with synapses throughout life. In past 20 years, numerous astrocyte-derived molecules promoting synaptogenesis have been discovered. However, our understanding cell biological basis underlying intra-neuron processes astrocyte-mediated is still in its infancy. Here, we provide a comprehensive overview various ways astrocytes talk neurons, highlight astrocytes’ heterogeneity that allow them displays regional-specific capabilities boosting synaptogenesis. Finally, conclude promises future directions on how organoids generated from human induced pluripotent stem cells (hiPSCs) effectively address signaling pathways employ synaptic development.

Язык: Английский

High-content CRISPR screening DOI Open Access
Christoph Bock, Paul Datlinger, Florence M. Chardon

и другие.

Nature Reviews Methods Primers, Год журнала: 2022, Номер 2(1)

Опубликована: Фев. 10, 2022

Язык: Английский

Процитировано

384

High-content CRISPR screening DOI
Christoph Bock, Paul Datlinger, Florence M. Chardon

и другие.

Nature Reviews Methods Primers, Год журнала: 2022, Номер 2(1)

Опубликована: Фев. 10, 2022

Язык: Английский

Процитировано

210

Human brain organogenesis: Toward a cellular understanding of development and disease DOI Creative Commons

Kevin W. Kelley,

Sergiu P. Paşca

Cell, Год журнала: 2021, Номер 185(1), С. 42 - 61

Опубликована: Ноя. 11, 2021

Язык: Английский

Процитировано

180

Human cerebral organoids — a new tool for clinical neurology research DOI Open Access
Oliver L. Eichmüller, Juergen A. Knoblich

Nature Reviews Neurology, Год журнала: 2022, Номер 18(11), С. 661 - 680

Опубликована: Окт. 17, 2022

Язык: Английский

Процитировано

176

Challenges of Organoid Research DOI Open Access
Madeline G. Andrews, Arnold R. Kriegstein

Annual Review of Neuroscience, Год журнала: 2022, Номер 45(1), С. 23 - 39

Опубликована: Янв. 5, 2022

Organoids are 3D cell culture systems derived from human pluripotent stem cells that contain tissue resident types and reflect features of early organization. Neural organoids a particularly innovative scientific advance given the lack accessibility developing brain intractability neurological diseases. have become an invaluable approach to model development not well reflected in animal models. also hold promise for study atypical cellular, molecular, genetic underscore Additionally, may provide platform testing therapeutics potential source replacement approaches injury or disease. Despite promising organoids, their broad utility is tempered by variety limitations yet be overcome, including high-fidelity types, limited maturation, physiology, arealization, limit reliability certain applications.

Язык: Английский

Процитировано

148

Single-cell brain organoid screening identifies developmental defects in autism DOI Creative Commons
Chong Li, Jonas Simon Fleck, Catarina Martins‐Costa

и другие.

Nature, Год журнала: 2023, Номер 621(7978), С. 373 - 380

Опубликована: Сен. 13, 2023

Abstract The development of the human brain involves unique processes (not observed in many other species) that can contribute to neurodevelopmental disorders 1–4 . Cerebral organoids enable study a context. We have developed CRISPR–human organoids–single-cell RNA sequencing (CHOOSE) system, which uses verified pairs guide RNAs, inducible CRISPR–Cas9-based genetic disruption and single-cell transcriptomics for pooled loss-of-function screening mosaic organoids. Here we show perturbation 36 high-risk autism spectrum disorder genes related transcriptional regulation uncovers their effects on cell fate determination. find dorsal intermediate progenitors, ventral progenitors upper-layer excitatory neurons are among most vulnerable types. construct developmental gene regulatory network cerebral from transcriptomes chromatin modalities identify disorder-associated perturbation-enriched modules. Perturbing members BRG1/BRM-associated factor (BAF) remodelling complex leads enrichment telencephalon progenitors. Specifically, mutating BAF subunit ARID1B affects transition oligodendrocyte interneuron precursor cells, phenotype confirmed patient-specific induced pluripotent stem cell-derived Our paves way high-throughput phenotypic characterization disease susceptibility organoid models with state, molecular pathway readouts.

Язык: Английский

Процитировано

137

Organoids: The current status and biomedical applications DOI Creative Commons
Siqi Yang, Hai‐Jie Hu, Heng‐Chung Kung

и другие.

MedComm, Год журнала: 2023, Номер 4(3)

Опубликована: Май 17, 2023

Organoids are three-dimensional (3D) miniaturized versions of organs or tissues that derived from cells with stem potential and can self-organize differentiate into 3D cell masses, recapitulating the morphology functions their in vivo counterparts. Organoid culture is an emerging technology, organoids various tissues, such as brain, lung, heart, liver, kidney, have been generated. Compared traditional bidimensional culture, organoid systems unique advantage conserving parental gene expression mutation characteristics, well long-term maintenance function biological characteristics vitro. All these features open up new opportunities for drug discovery, large-scale screening, precision medicine. Another major application disease modeling, especially hereditary diseases difficult to model vitro modeled by combining genome editing technologies. Herein, we introduce development current advances technology field. We focus on applications basic biology clinical research, also highlight limitations future perspectives. hope this review provide a valuable reference developments organoids.

Язык: Английский

Процитировано

112

Human organoids: New strategies and methods for analyzing human development and disease DOI Creative Commons
Nina S. Corsini, Juergen A. Knoblich

Cell, Год журнала: 2022, Номер 185(15), С. 2756 - 2769

Опубликована: Июль 1, 2022

For decades, insight into fundamental principles of human biology and disease has been obtained primarily by experiments in animal models. While this allowed researchers to understand many biological processes great detail, some developmental mechanisms have proven difficult study due inherent species differences. The advent organoid technology more than 10 years ago established laboratory-grown organ tissues as an additional model system recapitulate human-specific aspects biology. use 3D organoids, well other advances single-cell technologies, revealed unprecedented insights mechanisms, especially those that distinguish humans from species. This review highlights novel with a focus on how generated better understanding development disease.

Язык: Английский

Процитировано

106

Genetics of human brain development DOI
Yi Zhou, Hongjun Song, Guo‐li Ming

и другие.

Nature Reviews Genetics, Год журнала: 2023, Номер 25(1), С. 26 - 45

Опубликована: Июль 28, 2023

Язык: Английский

Процитировано

74

Assembloid CRISPR screens reveal impact of disease genes in human neurodevelopment DOI Creative Commons
Xiang-Ling Meng,

David Yao,

Kent Imaizumi

и другие.

Nature, Год журнала: 2023, Номер 622(7982), С. 359 - 366

Опубликована: Сен. 27, 2023

Abstract The assembly of cortical circuits involves the generation and migration interneurons from ventral to dorsal forebrain 1–3 , which has been challenging study at inaccessible stages late gestation early postnatal human development 4 . Autism spectrum disorder other neurodevelopmental disorders (NDDs) have associated with abnormal interneuron 5 but these NDD genes affect migration, how they mediate effects remains unknown. We previously developed a platform in subpallial organoids assembloids 6 Here we integrate CRISPR screening investigate involvement 425 development. first screen aimed revealed 13 candidate genes, including CSDE1 SMAD4 subsequently conducted an more than 1,000 that identified 33 cytoskeleton-related endoplasmic reticulum-related gene LNPK discovered that, during reticulum is displaced along leading neuronal branch before nuclear translocation. deletion interfered this displacement resulted migration. These results highlight power CRISPR-assembloid systematically map onto reveal disease mechanisms.

Язык: Английский

Процитировано

72