CAR-based therapies: opportunities for immuno-medicine beyond cancer

Nature Metabolism, Год журнала: 2022, Номер 4(2), С. 163 - 169

Опубликована: Фев. 28, 2022

Язык: Английский

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The 60-year evolution of lipid nanoparticles for nucleic acid delivery

Nature Reviews Drug Discovery, Год журнала: 2024, Номер 23(9), С. 709 - 722

Опубликована: Июль 4, 2024

Язык: Английский

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In vivo human T cell engineering with enveloped delivery vehicles

Nature Biotechnology, Год журнала: 2024, Номер 42(11), С. 1684 - 1692

Опубликована: Янв. 11, 2024

Viruses and virally derived particles have the intrinsic capacity to deliver molecules cells, but difficulty of readily altering cell-type selectivity has hindered their use for therapeutic delivery. Here, we show that cell surface marker recognition by antibody fragments displayed on membrane-derived encapsulating CRISPR-Cas9 protein guide RNA can genome editing tools specific cells. Compared conventional vectors like adeno-associated virus rely evolved capsid tropisms encoded cargo, these Cas9-packaging enveloped delivery vehicles (Cas9-EDVs) leverage predictable antibody-antigen interactions transiently machinery selectively cells interest. Antibody-targeted Cas9-EDVs preferentially confer in cognate target over bystander mixed populations, both ex vivo vivo. By using multiplexed targeting direct human T enable generation genome-edited chimeric antigen receptor humanized mice, establishing a programmable modality with potential widespread utility.

Язык: Английский

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Targeted modulation of immune cells and tissues using engineered biomaterials

Nature Reviews Bioengineering, Год журнала: 2023, Номер 1(2), С. 107 - 124

Опубликована: Янв. 30, 2023

Язык: Английский

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RNA therapeutics: updates and future potential

Science China Life Sciences, Год журнала: 2022, Номер 66(1), С. 12 - 30

Опубликована: Сен. 8, 2022

Язык: Английский

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