Evolutionary dynamics of genome size and content during the adaptive radiation of Heliconiini butterflies

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Сен. 12, 2023

Heliconius butterflies, a speciose genus of Müllerian mimics, represent classic example an adaptive radiation that includes range derived dietary, life history, physiological and neural traits. However, key lineages within the genus, across broader Heliconiini tribe, lack genomic resources, limiting our understanding how neutral processes shaped genome evolution during their radiation. Here, we generate highly contiguous assemblies for nine Heliconiini, 29 additional reference-assembled genomes, improve 10 existing assemblies. Altogether, provide dataset annotated genomes total 63 species, including 58 species tribe. We use this extensive to robust dated heliconiine phylogeny, describe major patterns introgression, explore architecture, basis innovations in enigmatic group, assessment putative regulatory regions at stem. Our work illustrates increased resolution provided by such dense sampling improves power test gene-phenotype hypotheses, precisely characterize evolve.

Язык: Английский

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Evolutionary origin of genomic structural variations in domestic yaks

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Сен. 19, 2023

Yak has been subject to natural selection, human domestication and interspecific introgression during its evolution. However, genetic variants favored by each of these processes have not distinguished previously. We constructed a graph-genome for 47 genomes 7 cross-fertile bovine species. This allowed detection 57,432 high-resolution structural (SVs) within across the species, which were genotyped in 386 individuals. evolutionary origins diverse SVs domestic yaks phylogenetic analyses. further identified 334 genes overlapping with that bore potential signals selection from wild yaks, plus an additional 686 introgressed cattle. Nearly 90% Introgression SV spanning KIT gene triggered breeding white yaks. validated significant association selected stratified expression, contributes phenotypic variations. Our results highlight different contribute diversity

Язык: Английский

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Adaptive introgression of a visual preference gene

Science, Год журнала: 2024, Номер 383(6689), С. 1368 - 1373

Опубликована: Март 21, 2024

Visual preferences are important drivers of mate choice and sexual selection, but little is known how they evolve at the genetic level. In this study, we took advantage diversity bright warning patterns displayed by Heliconius butterflies, which also used during choice. Combining behavioral, population genomic, expression analyses, show that two species have evolved same for red exchanging material through hybridization. Neural regucalcin1 correlates with visual preference across populations, disruption CRISPR-Cas9 impairs courtship toward conspecific females, providing a direct link between gene behavior. Our results support role hybridization behavioral evolution visually guided behaviors contributing to adaptation speciation encoded within genome.

Язык: Английский

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A long noncoding RNA at the cortex locus controls adaptive coloration in butterflies

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(36)

Опубликована: Авг. 30, 2024

Evolutionary variation in the wing pigmentation of butterflies and moths offers striking examples adaptation by crypsis mimicry. The

Язык: Английский

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A long non-coding RNA at thecortexlocus controls adaptive colouration in butterflies

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 12, 2024

Abstract Evolutionary variation in the wing pigmentation of butterflies and moths offers striking examples adaptation by crypsis mimicry. The cortex locus has been independently mapped as controlling colour polymorphisms 14 lepidopteran species, suggesting it acts a genomic hotspot for diversification patterns, but functional validation through protein-coding knockouts proven difficult to obtain. Our study unveils role novel long non-coding RNA (lncRNA) which we name ivory , transcribed from locus, modulating patterning butterflies. Strikingly, expression prefigures most melanic patterns during pupal development, an early developmental specifying scale identity. To test this, generated CRISPR mosaic knock-outs five nymphalid butterfly species show that mutagenesis yields transformations dark pigmented scales into white or light-coloured scales. Genotyping Vanessa cardui germline mutants associates these phenotypes small on-target deletions at conserved first exon . In contrast, mutant with confirmed null alleles lack any phenotype, exclude this adjacent gene. Overall, results lncRNA master switch pattern specification, played key roles adaptive Significance statement Deciphering genetic underpinnings is fundamental comprehensive understanding evolutionary processes. Long RNAs (lncRNAs) represent emerging category modulators within genome, yet they have overlooked source phenotypic diversity. study, unveil pivotal orchestrating transitions between light development. Remarkably, gene nested region known control multiple cases moths, including iconic natural selection. These findings highlight significant influence lncRNAs regulation, also underscore their potential players process itself.

Язык: Английский

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Multi-omics analyzes of Rosa gigantea illuminate tea scent biosynthesis and release mechanisms

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Сен. 30, 2024

Rose is an important ornamental crop cultivated globally for perfume production. However, our understanding of the mechanisms underlying scent production and molecular breeding fragrance hindered by lack a reference genome tea roses. We present first complete telomere-to-telomere (T2T) Rosa gigantea, with high quality (QV > 60), including detailed characterization structural features repetitive regions. The expansion genes associated phenylpropanoid biosynthesis may account unique scent. uncover release rhythm aromatic volatile organic compounds their gene regulatory networks through comparative genomics time-ordered co-expression networks. Analyzes eugenol homologs demonstrate how plants attract pollinators using specialized phenylpropanoids in specific tissues. This study highlights conservation utilization genetic diversity from wild endangered species multi-omics approaches, providing scientific foundation enhancing rose via de novo domestication.

Язык: Английский

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The Developmental Origin of Novel Complex Morphological Traits in Lepidoptera

Annual Review of Entomology, Год журнала: 2025, Номер 70(1), С. 421 - 439

Опубликована: Янв. 28, 2025

Novel traits in the order Lepidoptera include prolegs abdomen of larvae, scales, and eyespot band color patterns wings adults. We review recent work that investigates developmental origin diversification these four from a gene-regulatory network (GRN) perspective. While eyespots appear to derive distinct ancestral GRNs co-opted novel body regions, scales situ modifications sensory bristle GRN. The basal central symmetry systems bands on wing is associated with expression WntA gene those whereas more marginal depend two other genes, Distal-less spalt . Finally, several genes have been discovered play important roles regulating background color, via regulation pigmentation GRNs. identification shared cis- regulatory elements belonging helps trace evolutionary history traits. Future should examine extent which are co-opted/modified produce how map specific cell types derived

Язык: Английский

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Genetic basis of an adaptive polymorphism controlling butterfly silver iridescence

Current Biology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Identifying the genes and mutations that drive phenotypic variation which are subject to selection is crucial for understanding evolutionary processes. Mormon Fritillary butterflies (Speyeria mormonia) exhibit a striking wing color polymorphism throughout their range: typical morphs bear silver spots on ventral surfaces can co-occur with unsilvered displaying dull coloration.1 Through genome-wide association studies in two polymorphic populations, we fine-map this difference silvering 3' region of transcription factor gene optix. The expression optix confined regions surround spots, these patterns transformed identity upon RNA interference (RNAi) knockdown, implicating as repressor scales butterfly. We show haplotype shows signatures recent selective sweeps allele shared an population Speyeria hydaspe, suggesting introgressions may facilitate exchange variants adaptive potential across species. Remarkably, findings parallel role allelic sharing cis-regulatory modulation shaping aposematic red Heliconius butterflies,2,3,4,5,6,7 lineage separated from 45 million years ago.8 genetic basis thus be more predictable than often presumed, even traits appear divergent large distances.

Язык: Английский

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A distinct foliar pigmentation pattern formed by activator-repressor gradients upstream of an anthocyanin-activating R2R3-MYB

Cell Reports, Год журнала: 2024, Номер 43(7), С. 114444 - 114444

Опубликована: Июль 1, 2024

The emergence of novel traits is often preceded by a potentiation phase, when all the genetic components necessary for producing trait are assembled. However, elucidating these potentiating factors challenging. We have previously shown that an anthocyanin-activating R2R3-MYB, STRIPY, triggers distinct foliar pigmentation pattern in monkeyflower Mimulus verbenaceus. Here, using forward and reverse genetics approaches, we identify three STRIPY expression: MvHY5, master regulator light signaling activates expressed throughout leaf, two leaf developmental regulators, MvALOG1 MvTCP5, opposing gradients along proximodistal axis negatively regulate STRIPY. These results provide strong empirical evidence phenotypic novelties can be potentiated through incorporation into preexisting regulatory networks highlight importance positional information patterning stripe.

Язык: Английский

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Hierarchical morphogenesis of swallowtail butterfly wing scale nanostructures

eLife, Год журнала: 2023, Номер 12

Опубликована: Авг. 11, 2023

The study of color patterns in the animal integument is a fundamental question biology, with many lepidopteran species being exemplary models this endeavor due to their relative simplicity and elegance. While significant advances have been made unraveling cellular molecular basis pigmentary coloration, morphogenesis wing scale nanostructures involved structural production not well understood. Contemporary research on topic largely focuses few nymphalid model taxa (e.g., Bicyclus, Heliconius), despite an overwhelming diversity hierarchical nanostructural organization scales. Here, we present time-resolved, comparative developmental Parides eurimedes five other papilionid species. Our results uphold putative conserved role F-actin bundles acting as spacers between developing ridges, previously documented several Interestingly, while ridges are P. eurimedes, plasma membrane manifests irregular mesh-like crossribs characteristic Papilionidae, which delineate accretion cuticle into rows planar disks ridges. Once grown, disintegrating appear reorganize network that supports invagination underlying disks, subsequently forming extruded honeycomb lattice. uncover undocumented for complex nanostructures, likely specific Papilionidae.

Язык: Английский

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