Synergistic actions of three MYB transcription factors underpin blotch formation in tree peony

PLANT PHYSIOLOGY, Год журнала: 2024, Номер 196(3), С. 1869 - 1886

Опубликована: Авг. 14, 2024

Abstract Blotches in floral organs attract pollinators and promote pollination success. Tree peony (Paeonia suffruticosa Andr.) is an internationally renowned cut flower with extremely high ornamental economic value. Blotch formation on P. petals predominantly attributed to anthocyanin accumulation. However, the endogenous regulation of blotch remains elusive. Here, we identified regulatory modules governing anthocyanin-mediated petals, which involves transcription factors PsMYB308, PsMYBPA2, PsMYB21. PsMYBPA2 activated PsF3H expression provide sufficient precursor substrate for biosynthesis. PsMYB21 both PsFLS expressions promoted flavonol The significantly nonblotch regions inhibited by competing biosynthesis substrates, while conversely, its low region formation. PsMYB308 PsDFR directly prevent Notably, a smaller area, decreased content, inhibition structural gene were observed PsMYBPA2-silenced opposite phenotypes PsMYB308-silenced PsMYB21-silenced petals. Additionally, interacted PsbHLH1-3, their intensity target genes was synergistically regulated PsMYBPA2-PsbHLH1-3 PsMYB308-PsbHLH1-3 complexes. also competitively bound PsbHLH1-3 fine-tune network overaccumulation regions. Overall, our study uncovers complex R2R3-MYB transcriptional that governs providing insights into molecular mechanisms underlying suffruticosa.

Язык: Английский

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Genetic and molecular mechanisms of reproductive isolation in the utilization of heterosis for breeding hybrid rice

Journal of genetics and genomics/Journal of Genetics and Genomics, Год журнала: 2024, Номер 51(6), С. 583 - 593

Опубликована: Фев. 5, 2024

Heterosis, also known as hybrid vigor, is commonly observed in rice crosses. The hybridization of species or subspecies exhibits robust however, the direct harnessing this vigor hindered by reproductive isolation. Here, we review recent advances understanding molecular mechanisms governing isolation inter-subspecific and inter-specific hybrids. This encompasses genetic model within among Oryza sativa species, emphasizing essential role mitochondria process. Additionally, delve into intricacies interaction between autophagesome, elucidating their significant contribution to Furthermore, our exploration extends comprehending evolutionary dynamics speciation rice. Building on these advances, offer a forward-looking perspective how overcome challenges facilitate utilization heterosis future breeding endeavors.

Язык: Английский

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A micro-RNA is the effector gene of a classic evolutionary hotspot locus

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 12, 2024

In Lepidoptera (butterflies and moths), a genomic region around the gene cortex is hotspot locus, repeatedly used to generate intraspecific melanic wing color polymorphisms across 100-million-years of evolution. However, identity effector regulating within this locus remains unknown. Here, we show that none four candidate protein-coding genes including cortex, serve as major effectors. Instead, micro-RNA (miRNA), mir-193, serves three deeply diverged lineages butterflies, its function conserved in Drosophila. Lepidoptera, mir-193 derived from gigantic long non-coding RNA, ivory, it functions by directly repressing multiple pigmentation genes. We miRNA can drive repeated instances adaptive evolution animals.

Язык: Английский

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A long noncoding RNA at the cortex locus controls adaptive coloration in butterflies

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(36)

Опубликована: Авг. 30, 2024

Evolutionary variation in the wing pigmentation of butterflies and moths offers striking examples adaptation by crypsis mimicry. The

Язык: Английский

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The Ecology of Hybrid Incompatibilities

Cold Spring Harbor Perspectives in Biology, Год журнала: 2023, Номер 16(9), С. a041440 - a041440

Опубликована: Дек. 27, 2023

Ecologically mediated selection against hybrids, caused by hybrid phenotypes fitting poorly into available niches, is typically viewed as distinct from epistatic Dobzhansky-Muller incompatibilities. Here, we show how transgressive in hybrids manifests incompatibility. After outlining our logic, summarize current approaches for studying ecology-based on hybrids. We then quantitatively review QTL-mapping studies and find traits differing between parent taxa are polygenic. Next, describe verbal models of translate to phenotypic genetic fitness landscapes, highlighting emerging detecting polygenic Finally, a synthesis published data, report that trait transgression-and thus possibly extrinsic incompatibility hybrids-escalates with the divergence parents. discuss conceptual implications conclude ecological basis will facilitate new discoveries about mechanisms speciation.

Язык: Английский

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A long non-coding RNA at thecortexlocus controls adaptive colouration in butterflies

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 12, 2024

Abstract Evolutionary variation in the wing pigmentation of butterflies and moths offers striking examples adaptation by crypsis mimicry. The cortex locus has been independently mapped as controlling colour polymorphisms 14 lepidopteran species, suggesting it acts a genomic hotspot for diversification patterns, but functional validation through protein-coding knockouts proven difficult to obtain. Our study unveils role novel long non-coding RNA (lncRNA) which we name ivory , transcribed from locus, modulating patterning butterflies. Strikingly, expression prefigures most melanic patterns during pupal development, an early developmental specifying scale identity. To test this, generated CRISPR mosaic knock-outs five nymphalid butterfly species show that mutagenesis yields transformations dark pigmented scales into white or light-coloured scales. Genotyping Vanessa cardui germline mutants associates these phenotypes small on-target deletions at conserved first exon . In contrast, mutant with confirmed null alleles lack any phenotype, exclude this adjacent gene. Overall, results lncRNA master switch pattern specification, played key roles adaptive Significance statement Deciphering genetic underpinnings is fundamental comprehensive understanding evolutionary processes. Long RNAs (lncRNAs) represent emerging category modulators within genome, yet they have overlooked source phenotypic diversity. study, unveil pivotal orchestrating transitions between light development. Remarkably, gene nested region known control multiple cases moths, including iconic natural selection. These findings highlight significant influence lncRNAs regulation, also underscore their potential players process itself.

Язык: Английский

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For a Colorful Life: Recent Advances in Anthocyanin Biosynthesis during Leaf Senescence

Biology, Год журнала: 2024, Номер 13(5), С. 329 - 329

Опубликована: Май 9, 2024

Leaf senescence is the last stage of leaf development, and it accompanied by a color change. In some species, anthocyanins are accumulated during senescence, which vital indicators for both ornamental commercial value. Therefore, essential to understand molecular mechanism anthocyanin accumulation would provide new insight into autumn coloration breeding more colorful plants. Anthocyanin surprisingly complex process, significant advances have been made in past decades. this review, we focused on senescence. We emphatically discussed several networks linked genetic, hormonal, environmental, nutritional factors regulating This paper aims regulatory model put forward prospects future development.

Язык: Английский

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Exploring the Evolvability of Plant Specialized Metabolism: Uniqueness Out Of Uniformity and Uniqueness Behind Uniformity

Plant and Cell Physiology, Год журнала: 2023, Номер 64(12), С. 1449 - 1465

Опубликована: Июнь 12, 2023

The huge structural diversity exhibited by plant specialized metabolites has primarily been considered to result from the catalytic specificity of their biosynthetic enzymes. Accordingly, enzyme gene multiplication and functional differentiation through spontaneous mutations have established as molecular mechanisms that drive metabolic evolution. Nevertheless, how plants assembled maintained such genes typical clusters are observed in genomes, well why identical often exist phylogenetically remote lineages, is currently only poorly explained a concept known convergent Here, we compile recent knowledge on co-presence modules common kingdom but evolved under specific historical contextual constraints defined physicochemical properties each metabolite genetic presets genes. Furthermore, discuss manner generate uncommon (uniqueness out uniformity) an behind uniformity). This review describes emerging aspects evolvability metabolism underlie vast nature.

Язык: Английский

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TheivorylncRNA regulates seasonal color patterns in buckeye butterflies

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 12, 2024

Abstract Long non-coding RNAs (lncRNAs) are transcribed elements increasingly recognized for their roles in regulating gene expression. Thus far, however, we have little understanding of how lncRNAs contribute to evolution and adaptation. Here show that a conserved lncRNA, ivory , is an important color patterning the buckeye butterfly Junonia coenia . overlaps with cortex locus linked multiple cases crypsis mimicry Lepidoptera. Along companion paper by Livraghi et. al., argue not pattern interest at this locus. In J. cluster cis -regulatory (CREs) first intron genetically associated natural variation seasonal plasticity, targeted deletions these CREs phenocopy phenotypes. Deletions different produce other distinct phenotypes as well, including loss melanic eyespot rings, positive negative changes overall wing pigmentation. We transcription factors Spineless, Bric-a-brac, Ftz-f1 bind promoter during development, suggesting they directly regulate This case study demonstrates -regulation single RNA can exert diverse nuanced effects on development patterns, modulating seasonally plastic patterns. Significance The genomic hosting has been numerous adaptation moths butterflies, crypsis, mimicry, polyphenism. butterflies actual evolutionarily long (lncRNA), dubbed Compared genes, stands out because highly nuanced, quantitative pigmentation be achieved manipulating adjacent sequences. highlights underlying morphological evolution, emphasizes importance considering transcripts comparative genomics.

Язык: Английский

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Unraveling the regulatory network of flower coloration in soybean: Insights into roles of GmMYBA3 and GmMYBR1

The Crop Journal, Год журнала: 2024, Номер 12(2), С. 443 - 455

Опубликована: Март 7, 2024

Anthocyanins play crucial roles in pollen protection and pollinator attraction flowering plants. However, the mechanisms underlying flower color determination whether floral anthocyanin regulators participate other processes remain largely unresolved soybeans (Glycine max). In this study, we investigated genetic components governing biosynthesis soybean flowers. Molecular studies have characterized two antagonistic regulators, positive activator GmMYBA3 negative repressor GmMYBR1, that modulate gene expression of Further findings revealed a regulatory interplay between GmMYBR1 bridged by GmTT8a, highlighting complexity regulation different organs. Exploration additional cultivars demonstrated universality regulating biosynthesis- related genes, with GmF3′5′H identified as determinant white color. This study provides molecular mechanism determination, paving way for modification flowers to probably enhance their resistance abiotic stresses attractiveness pollinators.

Язык: Английский

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