Advanced Agrochem, Год журнала: 2024, Номер 4(1), С. 1 - 4
Опубликована: Сен. 19, 2024
Язык: Английский
Natural Product Reports, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
Discovery and scalable production of natural product drugs require a combination molecular biology, data science, chemistry fermentation methods. Here, we describe our drug discovery toolbox share perspective on future directions.
Язык: Английский
Процитировано
9
Journal of Experimental Botany, Год журнала: 2024, Номер unknown
Опубликована: Июль 19, 2024
Abstract Plants are unique organisms that have developed ingenious strategies to cope with environmental challenges, such as herbivorous insects. One of these is the synthesis a vast array chemical compounds, known specialized metabolites, serve many ecological functions. Among most fascinating and diverse groups metabolites alkaloids, which characterized by presence nitrogen atom within heterocyclic ring. While some medicinal recreational applications, others highly unpalatable and/or toxic. The effects alkaloids on both humans insects can be very diverse, affecting their physiology behavior. Insects feed alkaloid-containing plants evolved various mechanisms consequences toxins. These include sequestration, where store in tissues or organs, enzymatic detoxification through enzymes cytochrome P450 monooxygenases glutathione S-transferases, behavioral adaptations selective feeding. In this review, we explore relationships between plant evolutionary enable exploit alkaloid-rich food sources niches minimizing harmful natural compounds. We aim provide comprehensive updated overview complex interaction.
Язык: Английский
Процитировано
4
Nachrichten aus der Chemie, Год журнала: 2025, Номер 73(3), С. 40 - 70
Опубликована: Фев. 28, 2025
Abstract Highlights von November 2023 bis 2024: die erste Einelektron‐C–C‐σ‐Bindung und Anti‐Bredt‐Verbindung; gesättigte Heterocyclen elektrochemisch funktionalisieren; Ausrichten diskotischer Flüssigkristalle; enantioselektive Wagner‐Meerwein‐Umlagerung reiner Aliphaten; photokatalytisch Furanen zu Pyrrolen; mit Ammoniak primären Arylaminen; Metallschrott recyceln ionischen Flüssigkeiten; terminale Alkene Ni‐Katalysatoren zum (Z)‐ oder (E)‐Alken isomerisieren;neue Fungizide, Medikamente Alkaloide.
Процитировано
0
Chemical Reviews, Год журнала: 2025, Номер unknown
Опубликована: Март 21, 2025
Synthetic biology has played an important role in the renaissance of natural products research during post-genomics era. The development and integration new tools have transformed workflow product discovery engineering, generating multidisciplinary interest field. In this review, we summarize recent developments biosynthesis from three different aspects. First, advances bioinformatics, experimental, analytical to identify associated with predicted biosynthetic gene clusters (BGCs) will be covered. This followed by extensive review on heterologous expression bacterial, fungal plant organisms. native host-independent paradigm identification, pathway characterization, enzyme is where synthetic most prominent role. Lastly, strategies engineer pathways for structural diversification complexity generation discussed, including assembly-line megasynthase precursor-directed modification, combinatorial biosynthesis.
Язык: Английский
Процитировано
0
Journal of the American Chemical Society, Год журнала: 2025, Номер unknown
Опубликована: Апрель 3, 2025
Imines and enamines are useful intermediates in biochemical aldol-type reactions since their acid/base characteristics accessible near neutrality. However, the generation of aldimines water is a challenge as equilibrium favors components alkyl amines aldehydes. Here, we report generation, recognition, protection accomplished by water-soluble capsule. The capsule provides well-defined hydrophobic cavity isolating from medium dry seam Se-N chalcogen bonds instead wet hydrogen water. A series diverse formed situ trapped rapidly (within 45 min at mM concentrations) D2O with more than 90% yields. encapsulated stable for least one month under ambient conditions. aliphatic selectively even though they less aromatic imines solution. can be released containers competitive guests such adamantane.
Язык: Английский
Процитировано
0
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(9), С. 4425 - 4425
Опубликована: Май 6, 2025
Buckwheat is a promising crop with grains that are rich in nutrients and bioactive compounds. Genome sequence data for common Tartary buckwheat have recently become available. Currently, there critical need the development of simple reliable transient gene expression protocol, as well stable genetic transformation method, to facilitate metabolic engineering compounds, functional analysis genes, targeted editing, and, long-term perspective, accelerate breeding process buckwheat. In this paper, we report optimized methods Agrobacterium-mediated Fagopyrum esculentum F. tartaricum. Leaf cotyledon tissues were infiltrated an A. tumefaciens-bearing construct containing eGFP GUS reporter genes. Histochemical staining Western blotting used confirm proteins. We also demonstrate usefulness developed method gramine biosynthetic pathway HvAMIS HvNMT genes transiently expressed leaves, de novo production was confirmed by LC-MS. Moreover, planta (eGFP) selectable marker (NptII) achieved vacuum infiltration. Genomic integration polymerase chain reaction (PCR), whereas fluorescence microscopy.
Язык: Английский
Процитировано
0
Journal of the American Chemical Society, Год журнала: 2025, Номер unknown
Опубликована: Май 9, 2025
The demand for novel enzyme-catalyzed reactions in chemical synthesis has spurred the development of many new-to-nature reactions. Additionally, detailed analysis biosynthetic pathways can uncover unprecedented chemical/enzymatic mechanisms. In this study, we revisited catalytic mechanism 2-oxoglutarate-dependent dioxygenase Pt2OGD-1, involved biosynthesis huperzine alkaloids. Our experimental and computational investigations uncovered a previously unknown enzymatic C-C bond cleavage piperidine ring alkaloid scaffold, resembling an oxidative retro-aza-Prins reaction. Here, transformation is initiated by hydrogen abstraction, followed electron transfer at 4-position heterocycle, triggering opening finally resulting loss carbon atom as formaldehyde. This discovery expands toolbox reactions, enhances our understanding these enzymes, may facilitate their application biotechnological production pharmaceutically relevant scaffolds well biocatalysts with similar activities.
Язык: Английский
Процитировано
0
Current Opinion in Plant Biology, Год журнала: 2024, Номер 82, С. 102638 - 102638
Опубликована: Сен. 25, 2024
Язык: Английский
Процитировано
2
Current Opinion in Plant Biology, Год журнала: 2024, Номер 80, С. 102554 - 102554
Опубликована: Май 30, 2024
Elucidating the biochemical pathways of specialised metabolites in plants is key to enable or improve their sustainable biotechnological production. Chemical tools can greatly facilitate discovery biosynthetic genes and enzymes. Here, we summarise transdisciplinary approaches where methods from chemistry chemical biology helped overcome challenges pathway elucidation. Based on recent examples, describe how state-of-the-art isotope labelling experiments guide selection gene candidates, affinity-based probes identification novel enzymes, semisynthesis availability elusive intermediates, biomimetic reactions provide a better understanding inherent reactivity. We anticipate that wider application such will accelerate pace elucidation plants.
Язык: Английский
Процитировано
1
ACS Bio & Med Chem Au, Год журнала: 2024, Номер 5(1), С. 16 - 30
Опубликована: Ноя. 27, 2024
Cytochrome P450 enzymes catalyze a large number of oxidative transformations that are responsible for natural product synthesis. Recent studies have revealed their unique ability to the formation C-N and C-S bonds, broadening biosynthetic applications. However, enzymatic mechanisms behind these reactions still unclear. This review focuses on theoretical insights into P450-catalyzed bond formation. The key roles conformational dynamics substrate radicals, influenced by enzyme environment, in modulating selectivity reactivity highlighted. Understanding reaction offers valuable guidance engineering design
Язык: Английский
Процитировано
1