Nature reviews. Immunology, Год журнала: 2024, Номер 24(6), С. 377 - 377
Опубликована: Апрель 24, 2024
Язык: Английский
Water Research, Год журнала: 2024, Номер 256, С. 121652 - 121652
Опубликована: Апрель 21, 2024
Язык: Английский
Процитировано
10
Nature, Год журнала: 2024, Номер 632(8026), С. 885 - 892
Опубликована: Авг. 7, 2024
Migration and homing of immune cells are critical for surveillance. Trafficking is mediated by combinations adhesion chemokine receptors that guide cells, in response to signals, specific locations within tissues the lymphatic system support tissue-localized reactions systemic immunity
Язык: Английский
Процитировано
6
Science, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 28, 2024
To elucidate aging-associated cellular population dynamics, we present PanSci , a single-cell transcriptome atlas profiling over 20 million cells from 623 mouse tissues across different life stages, sexes, and genotypes. This comprehensive dataset reveals more than 3,000 unique states 200 cell populations. Our panoramic analysis uncovered organ-, lineage-, sex-specific shifts of dynamics during lifespan progression. Moreover, identify both systematic organ-specific alterations in immune populations associated with aging. We further explored the regulatory roles system on aging pinpointed specific age-related expansions that are lymphocyte dependent. “cell-omics” strategy enhances comprehension lays groundwork for exploring complex networks diseases.
Язык: Английский
Процитировано
5
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown
Опубликована: Фев. 3, 2025
Abstract In eutherian mammals, the embryonic cloaca is partitioned into genitourinary and anorectal canals by urorectal septum. At caudal end of mouse embryo, septum contributes to perineum, which separates anus from external genitalia. Growth displaces cloacal endoderm surface where it incorporated epidermis, an enigmatic fate for endodermal cells. Here we show that cells differentiate true epidermis in expressing basal, spinous, granular cell markers. Endodermal lost through terminal differentiation desquamation postnatally, when replaced ectoderm. Live imaging single-cell tracking reveal ectodermal move at a faster velocity lateral-to-medial direction, converging towards narrow band between Although perineum sexually dimorphic, similar spatiotemporal patterns movement were observed males females. These results demonstrate differentiates non-renewing, transient midline perineum. Differential suggests develops convergent extension. findings provide foundation further studies development sex-specific epidermal phenotypes.
Язык: Английский
Процитировано
0
Science, Год журнала: 2024, Номер 384(6692), С. 159 - 160
Опубликована: Апрель 4, 2024
Androgen signaling skews skin immunity toward reduced inflammation in male mice.
Язык: Английский
Процитировано
4
Transplantation, Год журнала: 2025, Номер unknown
Опубликована: Янв. 9, 2025
Tissue-resident lymphocytes (TRLs) provide a front-line immunological defense mechanism uniquely placed to detect perturbations in tissue homeostasis. The heterogeneous TRL population spans the innate adaptive immune continuum, with roles during normal physiology homeostatic maintenance, repair, pathogen detection, and rapid mounting of responses. TRLs are especially enriched liver, every subset represented, including liver-resident natural killer cells; tissue-resident memory B conventional CD8, CD4, regulatory T unconventional gamma-delta, killer, mucosal-associated invariant cells. importance donor- recipient-derived after transplantation is becoming increasingly recognized, although it has not been examined detail liver transplantation. This review summarizes evidence for transplant immunology, focusing on their features, functions, potential harnessing improve outcomes.
Язык: Английский
Процитировано
0
Research Square (Research Square), Год журнала: 2025, Номер unknown
Опубликована: Янв. 14, 2025
Background Tuberculosis (TB) remains a major cause of global morbidity and mortality. Efforts to control TB are hampered by the lengthy cumbersome treatment required eradicate infection. Bacterial persistence during exposure bactericidal antibiotics is at least partially mediated bacterial stringent response enzyme, Rel Mtb . A therapeutic DNA vaccine targeting has been shown increase efficacy antitubercular drugs, fusing macrophage-inflammatory protein 3α (MIP-3α), which interacts with CCR6 on immature dendritic cells (iDCs), further increases vaccine's efficacy. secondary analysis these prior studies elucidated prominent sex-based differences in efficacy, female mice showing improved microbial outcomes compared males as result MIP-3α-Rel constructs, greater sex-associated difference group. In current study, we addressed hypothesis that sex-related due differential DC activation/function soon after vaccination. Methods EαGFP reporter model was used track antigen presentation an antibody Y-Ae binds Eα peptide tag complex I-A b MHC-II molecules. Results MIP-3α-EαGFP groups had more DCs presenting infiltrating from periphery, abundant Langerhans CD8 + CD103 cross-presenting dermal females. This also shows there activation, measured CD80 MHC II MFI, MIP-3α alone, especially mice. Conclusions Our findings consistent sex- MIP-3α-related seen supports both sexes enhances uptake cell activation peripheral iDCs. Additionally, Female showed levels presentation, able cross-present antigen, explaining why they best outcomes. Further understand underlying mechanisms link APC results directly T-cell responses.
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown
Опубликована: Янв. 22, 2025
Vertically transferred maternal cells or microchimeric (MMCs) engraft the fetus and persist in offspring for long periods of time. How altered immune states arising from infection affect MMCs their function is poorly understood. Here, we show that pregnancy-associated transient alters to differentially regulate immunity offspring. In male dams previously infected with Yersinia pseudotuberculosis , confer a pro-inflammatory type 17 T effector phenotype leads enhanced protective an unrelated Salmonella infection. Thus, acquired imprinted by microbial exposure during pregnancy exert antigen agnostic sex-differential effect on immunity, may potentially be targeted deliver benefits infants vulnerable early life period.
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown
Опубликована: Фев. 15, 2025
A diverse naive CD8 T cell repertoire is essential to provide broad protection against infection and cancer. Aging diminishes cells, reducing potential diversity leading lymph node contraction. Here, we revealed that this decline occurs earlier in males, resulting significant sex differences immunity during middle age. Earlier life, cells males become virtual memory prone premature senescence. Due androgen-driven thymic atrophy naïve are insufficiently replenished. Therapeutic thymus rejuvenation via testosterone ablation restored nodes of middle-aged male mice, enhanced tumor recognition. These findings show the crucial role age on repertoires suggest strategies restore immune function aging.
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown
Опубликована: Фев. 28, 2025
Lymph nodes are highly specialized immune organs that orchestrate the adaptive response. In lymph nodes, naïve B and T lymphocytes encounter cognate antigens, sparking their activation response to foreign substances. grow in an challenge, at least part accommodate increased numbers of infiltrating proliferating lymphocytes. This behavior is supported by a robust three-dimensional network extracellular matrix (ECM) fibers fibroblastic reticular cells (FRCs). ECM FRCs work synergistically alternate stretching contractile forces between them allowing node maintain structural integrity during rapid tissue reconstruction. These changes ultimately alter material properties node, which can impact cell migration, proliferation, differentiation. Recent has investigated physiological implications changing microenvironment; however, biophysical these remain largely unexplored. Here, we use multiple particle tracking microrheology (MPT), minimally invasive nanoparticle-based technique investigate (elastic/loss moduli, microviscosity, pore size) post inflammatory stimulus. Our results highlight mechanical both initial phases acute upon resolution inflammation, topic relatively understudied. We show rich areas restructure independently, with zones remodeling significantly exhibiting nearly 3-fold higher elastic modulus. Additionally, for first time, biological sex modulates biomechanics inflammation: from female mice showed ∼20-fold increase loss moduli peak while male had ∼5-fold decrease moduli. appeared permanently remodel inflammation resulting maintenance overall modulus, returned untreated nodes. also found some biomechanical were correlated materials suggesting structure-function relationship. Overall, our studies provide key insights into how altered previously unstudied area, lay foundation relationships involved demonstrate analysis interstitial they vary stimuli.
Язык: Английский
Процитировано
0