Virulence, Год журнала: 2024, Номер 16(1)
Опубликована: Дек. 26, 2024
Язык: Английский
Cell, Год журнала: 2024, Номер 187(19), С. 5121 - 5127
Опубликована: Сен. 1, 2024
Язык: Английский
Процитировано
17
Nature, Год журнала: 2025, Номер 638(8049), С. 49 - 57
Опубликована: Фев. 5, 2025
Язык: Английский
Процитировано
4
PeerJ, Год журнала: 2025, Номер 13, С. e19370 - e19370
Опубликована: Апрель 28, 2025
Genes are continually formed and lost as a genome evolves. However, new genes may tend to appear during specific evolutionary epochs rather than others, or disappear together in more recent organismal clade. Methods identify gene origination might simply use the last common ancestor contain an ortholog putative point, heuristic threshold that allows for certain amount of missing orthologs cohort species examined. Here, avoid such issues, alternative approach based on clustering phylogenetic profiles is applied, results examined any evidence epochal trends origination, associated sequence traits functional associations. A profile array indicating presence absence list species. These were compared clustered discern patterns occurrences across >800 fungal species, centering analysis budding yeast Saccharomyces cerevisiae. Clear observed linked ancestors Saccharomycetaceae Saccharomycetes, also Fungi earlier ancestors. independent proteome genome-assembly quality underlying data. Clusters demonstrated some significant associations, cellular spore formation chromosome segregation originating Saccharomycetaceae. The enabled detection parameter-independent intrinsic disorder, prion-like composition uniqueness function age. For example: proteins with domains have arisen at similar rate most evolution centred S. cerevisiae; mild disorder appeared early epoch recently, very recently least likely be single-copy (i.e., 'unique' proteins). individual proteins, cluster data generated here useful investigating experimental hypotheses, since they provide linkages yet discerned.
Язык: Английский
Процитировано
0
Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15
Опубликована: Май 9, 2025
Background Oral health is increasingly recognized as a crucial determinant of overall in people living with HIV/AIDS (PLWHA). Specifically, the oral mycobiome may play pivotal role HIV-associated complications. However, fungal species involved and their potential biomarkers for HIV-related conditions remain poorly understood. This study investigates salivary profiles PLWHA who have sex men (MSM), focusing on diversity, functional shifts, correlations disease progression. Methods A cross-sectional included 25 MSM participants divided into five groups: HIV-negative controls (n = 5) four HIV-positive groups stratified by CD4 count: Stage 0 (HIV RNA-positive/antibody-negative; n 5), 1 (CD4 ≥500 cells/μL; 2 200–499 3 <200 cells/μL or opportunistic infections; 5). Saliva samples were collected analyzed using metagenomic sequencing (Illumina NovaSeq platform). Bioinformatic analyses genome assembly (MEGAHIT), gene clustering (CD-HIT), abundance calculation (SOAPaligner), annotation (BLASTP), KEGG pathway (KOBAS 2.0). Statistical (Kruskal-Wallis tests, Spearman’s correlation) assessed associations between profiles, count, viral loads. Results total 51 genera identified, Pseudogymnoascus being most abundant. Functional analysis revealed 113 shared pathways, which 69 unique to 3, primarily related metabolic disease-related processes. Notably, Auricularia exhibited positive correlation count ( P ≤ 0.01), while Mucor showed negative 0.0299). Conclusions Salivary composition function shift significantly across HIV stages, reflecting immune decline. dominance challenges conventional views ecology immunocompromised hosts. These findings highlight mycobiome’s diagnostic monitoring health. Longitudinal studies are needed validate clinical relevance.
Язык: Английский
Процитировано
0
Journal of Fungi, Год журнала: 2025, Номер 11(5), С. 372 - 372
Опубликована: Май 13, 2025
Copper (Cu) is an essential metal micronutrient, and a fungal pathogen’s ability to thrive in diverse niches across broad range of bioavailable copper levels vital for host colonization propagation. Recent transcriptomic studies have implied that trace acquisition important the propagation white nose syndrome (WNS) causing fungus, Pseudogymnoascus destructans, on bat hosts. This report characterizes P. destructans transcriptional response Cu-withholding Cu-overload stress. We identify 583 differently expressed genes (DEGs) respond stress 667 DEGs find Cu-transporter CTR1a CTR1b, as well two homologs Cryptococcus neoformans Cbi1/BIM1 VC83_03095 (BLP2) VC83_07867 (BLP3), are highly regulated by cluster genes, VC83_01834 – VC83_01838, bioavailability, which we Cu-Responsive gene Cluster (CRC). chronic exposure elevated leads increase associated with DNA repair replication fidelity. A comparison our datasets at WNS infection sites reveals several putative virulence factors environmental
Язык: Английский
Процитировано
0
The Microbe, Год журнала: 2024, Номер 5, С. 100186 - 100186
Опубликована: Окт. 9, 2024
Язык: Английский
Процитировано
2
PLoS Pathogens, Год журнала: 2024, Номер 20(8), С. e1012342 - e1012342
Опубликована: Авг. 29, 2024
The emergence of white-nose syndrome (WNS) in North America has resulted mass mortalities hibernating bats and total extirpation local populations. need to mitigate this disease stirred a significant body research understand its pathogenesis. Pseudogymnoascus destructans, the causative agent WNS, is psychrophilic (cold-loving) fungus that resides within class Leotiomycetes, which contains mainly plant pathogens unrelated other consequential animals. In review, we revisit unique biology P. destructans provide an updated analysis stages mechanisms WNS progression. extreme life history bats, nature evolutionary distance from well-characterized animal-infecting fungi translate into host-pathogen interactions, many them yet be discovered.
Язык: Английский
Процитировано
1
Science, Год журнала: 2024, Номер 385(6705), С. 142 - 143
Опубликована: Июль 11, 2024
A fungus uses different cell entry strategies, depending on its host's hibernation status.
Язык: Английский
Процитировано
0
Virulence, Год журнала: 2024, Номер 16(1)
Опубликована: Дек. 26, 2024
Язык: Английский
Процитировано
0