Brain Structure and Function, Год журнала: 2025, Номер 230(5)
Опубликована: Май 28, 2025
Abstract The present study provides an in-depth neuroanatomical characterization of the brain Pseudopus apodus , combining magnetic resonance imaging (MRI) with histological analysis by immunofluorescence. In telencephalon, pallial regions showed distinct anatomical features, including a cortical structure, dorsal ventricular ridge and spherical nucleus, but prominent layering patterns, observable on slides, were not fully resolved MRI. Subpallial structures, such as nucleus accumbens basal ganglia, delineated clarity further supported hypothalamic diencephalic regions, dense complex cellular composition made precise delineation individual nuclei difficult MRI, in contrast to accuracy, however MRI identification major tracts running through these domains are clearly identifiable. Mesencephalic rhombencephalic optic tectum, isthmic nuclei, cerebellum, reticular groups, systematically described using combination techniques. addition, immunofluorescence specific markers, Calretinin, ChAT, Isl1, Satb1, Serotonin Tyrosine Hydroxylase, provided higher resolution functional sub-regions, allowing boundaries facilitating comprehensive regional mapping, showing organizational arrangements, both rostral crest, caudal within tegmental posterior brain, ventral area, substantia nigra raphe nuclei. These findings establish robust framework for contributing significantly understanding reptile organization providing valuable insights into evolutionary adaptations underlying limbless lizard neuroanatomy.
Язык: Английский