Scientific Reports,
Год журнала:
2021,
Номер
11(1)
Опубликована: Май 27, 2021
Abstract
Optical
stimulation
is
a
paradigm-shifting
approach
to
modulating
neural
activity
that
has
the
potential
overcome
issue
of
current
spread
occurs
with
electrical
by
providing
focused
stimuli.
But
optical
either
requires
high
power
infrared
light
or
genetic
modification
neurons
make
them
responsive
lower
visible
light.
This
work
examines
activation
auditory
following
optogenetic
via
AAV
injection
in
two
species
(mouse
and
guinea
pig).
An
Anc80
viral
vector
was
used
express
channelrhodopsin
variant
ChR2-H134R
fused
fluorescent
reporter
gene
under
control
human
synapsin-1
promoter.
The
administered
directly
cochlea
(n
=
33)
posterior
semi-circular
canal
C57BL/6
mice
4)
pig
cochleae
6).
Light
(488
nm),
stimuli
combination
these
(hybrid
stimulation)
delivered
laser-coupled
fibre
co-located
platinum
wire.
Activation
thresholds,
stimulus
interactions
were
obtained
from
multi-unit
recordings
central
nucleus
inferior
colliculus
injected
mice,
as
well
transgenic
4).
Expression
examined
histology.
In
mouse,
transduction
most
successful
when
at
neonatal
age
up
89%
transduced.
Auditory
neuron
transductions
not
pigs.
Inferior
responses
detected
cochleotopic
manner
all
expression.
There
significant
correlation
between
thresholds
higher
proportions
transduced
neurons.
no
difference
provided
light/electrical
delivery
system
here
(optical
bonded
25
µm
platinum/iridium
wire).
Hybrid
stimulation,
comprised
sub-threshold
‘prime’
raise
excitability
neurons,
lowered
threshold
for
cases,
but
impact
on
excitation
width
more
variable
compared
mice.
study
demonstrates
opsin
expression
levels
pattern
hybrid
considering
techniques
neuromodulation.
JASA Express Letters,
Год журнала:
2022,
Номер
2(7)
Опубликована: Июль 1, 2022
Cochlear
implants
have
been
the
most
successful
neural
prosthesis,
with
one
million
users
globally.
Researchers
used
source-filter
model
and
speech
vocoder
to
design
modern
multi-channel
implants,
allowing
implantees
achieve
70%–80%
correct
sentence
recognition
in
quiet,
on
average.
also
cochlear
implant
help
understand
basic
mechanisms
underlying
loudness,
pitch,
cortical
plasticity.
While
front-end
processing
advances
improved
noise,
unilateral
quiet
has
plateaued
since
early
1990s.
This
lack
of
progress
calls
for
action
re-designing
stimulating
interface
collaboration
general
neurotechnology
community.
Advanced Optical Materials,
Год журнала:
2021,
Номер
9(14)
Опубликована: Май 17, 2021
Abstract
As
solid‐state
light
sources
based
on
amorphous
organic
semiconductors,
light‐emitting
diodes
(OLEDs)
are
widely
used
in
modern
smartphone
displays
and
TVs.
Due
to
the
dramatic
improvements
stability,
efficiency,
brightness
achieved
over
last
three
decades,
OLEDs
have
also
become
attractive
for
compact
“imperceptible”
biomedical
devices
that
use
probe,
image,
manipulate,
or
treat
biological
matter.
The
inherent
mechanical
flexibility
of
their
compatibility
with
a
wide
range
substrates
geometries
particular
benefit
this
context.
Here,
recent
progress
development
applications
is
reviewed.
specific
requirements
poses
described
compared
current
state
art,
terms
brightness,
patterning,
encapsulation
OLEDs.
Examples
from
several
main
areas
then
discussed
some
detail:
on‐chip
sensing
integration
microfluidics,
wearable
optical
monitoring,
therapeutic
devices,
emerging
neuroscience
targeted
photostimulation
via
optogenetics.
review
closes
brief
outlook
future
avenues
scale
manufacturing
OLED‐based
use.
Nature Communications,
Год журнала:
2022,
Номер
13(1)
Опубликована: Фев. 11, 2022
Abstract
Optogenetic
methods
provide
efficient
cell-specific
modulations,
and
the
ability
of
simultaneous
neural
activation
inhibition
in
same
brain
region
freely
moving
animals
is
highly
desirable.
Here
we
report
bidirectional
neuronal
activity
manipulation
accomplished
by
a
wireless,
dual-color
optogenetic
probe
synergy
with
co-expression
two
spectrally
distinct
opsins
(ChrimsonR
stGtACR2)
rodent
model.
The
flexible
comprises
vertically
assembled,
thin-film
microscale
light-emitting
diodes
lateral
dimension
125
×
180
µm
2
,
showing
colocalized
red
blue
emissions
enabling
chronic
vivo
operations
desirable
biocompatibilities.
Red
or
irradiations
deterministically
evoke
silence
neurons
co-expressing
opsins.
interferes
dopaminergic
ventral
tegmental
area
mice,
increasing
decreasing
dopamine
levels.
Such
regulations
further
generate
rewarding
aversive
behaviors
interrogate
social
interactions
among
multiple
mice.
These
technologies
create
numerous
opportunities
implications
for
research.
Advanced Materials,
Год журнала:
2023,
Номер
35(42)
Опубликована: Сен. 19, 2023
Abstract
Optogenetic
modulation
of
brain
neural
activity
that
combines
optical
and
electrical
modes
in
a
unitary
system
has
recently
gained
robust
momentum.
Controlling
illumination
spatial
coverage,
designing
light‐activated
modulators,
developing
wireless
light
delivery
data
transmission
are
crucial
for
maximizing
the
use
neuromodulation.
To
this
end,
biocompatible
electrodes
with
enhanced
optoelectrical
performance,
device
integration
multiplexed
addressing,
transmission,
multimodal
operation
soft
systems
have
been
developed.
This
review
provides
an
outlook
uniformly
illuminating
large
areas
while
spatiotemporally
imaging
responses
upon
stimulation
little
artifacts.
Representative
concepts
important
breakthroughs,
such
as
head‐mounted
illumination,
multiple
implanted
fibers,
micro‐light‐delivery
devices,
discussed.
Examples
techniques
incorporate
electrophysiological
monitoring
presented.
Challenges
perspectives
posed
further
research
efforts
toward
high‐density
interface
modulation,
potential
nonpharmacological
neurological
disease
treatments
stimulation.
Annual Review of Neuroscience,
Год журнала:
2024,
Номер
47(1), С. 103 - 121
Опубликована: Апрель 10, 2024
The
cochlear
implant
(CI)
is
considered
the
most
successful
neuroprosthesis
as
it
enables
speech
comprehension
in
majority
of
million
otherwise
deaf
patients.
In
hearing
by
electrical
stimulation
auditory
nerve,
broad
spread
current
from
each
electrode
acts
a
bottleneck
that
limits
transfer
sound
frequency
information.
Hence,
there
remains
major
unmet
medical
need
for
improving
quality
with
CIs.
Recently,
optogenetic
cochlea
has
been
suggested
an
alternative
approach
restoration.
Cochlear
optogenetics
promises
to
more
information,
hence
hearing,
light
can
conveniently
be
confined
space
activate
nerve
within
smaller
tonotopic
ranges.
this
review,
we
discuss
latest
experimental
and
technological
developments
restoration
outline
remaining
challenges
en
route
clinical
translation.
EMBO Molecular Medicine,
Год журнала:
2021,
Номер
13(6)
Опубликована: Май 7, 2021
Optogenetic
stimulation
of
spiral
ganglion
neurons
(SGNs)
in
the
ear
provides
a
future
alternative
to
electrical
used
current
cochlear
implants.
Here,
we
employed
fast
and
very
variants
red-light-activated
channelrhodopsin
(ChR)
Chrimson
(f-Chrimson
vf-Chrimson)
study
their
utility
for
optogenetic
SGNs
mice.
The
light
requirements
were
higher
vf-Chrimson
than
f-Chrimson,
even
when
optimizing
membrane
expression
by
adding
potassium
channel
trafficking
sequences.
time
intensity
coding
single
putative
compared
with
acoustic
clicks.
enabled
fire
at
near-physiological
rates
good
temporal
precision
up
250
Hz
stimulation.
dynamic
range
SGN
spike
rate
upon
was
narrower
clicks
but
larger
reported
timing,
on
other
hand,
more
comparable
In
conclusion,
f-Chrimson
are
promising
candidates
auditory
research
Proceedings of the National Academy of Sciences,
Год журнала:
2021,
Номер
118(18)
Опубликована: Апрель 26, 2021
The
cochlea
of
our
auditory
system
is
an
intricate
structure
deeply
embedded
in
the
temporal
bone.
Compared
with
other
sensory
organs
such
as
eye,
has
remained
poorly
accessible
for
investigation,
example,
by
imaging.
This
limitation
also
concerns
further
development
technology
restoring
hearing
case
cochlear
dysfunction,
which
requires
quantitative
information
on
spatial
dimensions
and
sensorineural
status
cochlea.
Here,
we
employed
X-ray
phase-contrast
tomography
light-sheet
fluorescence
microscopy
their
combination
multiscale
multimodal
imaging
morphology
species
that
serve
established
animal
models
research.
We
provide
a
systematic
reference
morphological
parameters
relevant
implant
rodent
nonhuman
primate
models.
simulate
spread
light
from
emitters
optical
implants
within
reconstructed
cochlea,
indicates
spatially
narrow
optogenetic
excitation
spiral
ganglion
neurons.
