Microalgae and microbial inoculant as partial substitutes for chemical fertilizer enhance Polygala tenuifolia yield and quality by improving soil microorganisms

Frontiers in Plant Science, Год журнала: 2025, Номер 15

Опубликована: Янв. 16, 2025

Excessive utilization of chemical fertilizers degrades the quality medicinal plants and soil. Bio-organic (BOFs) including microbial inoculants microalgae have garnered considerable attention as potential substitutes for fertilizer to enhance yield. In this study, a field experiment was conducted investigate effects BOF partially substituting on growth plant Polygala tenuifolia. The parameters, bioactive component contents, soil properties composition rhizosphere microorganisms were measured. results indicated that 40% with showed most pronounced growth-promoting effect, leading 29.30% increase in underground biomass 19.72% 3,6'-disinapoylsucrose (DISS) content. Substituting 20% improved quality, significantly increasing organic matter content by 15.68% (p<0.05). Microalgae addition affected bacterial community P. tenuifolia, reducing relative abundance Cladosporium 33.33% 57.93%, while Chloroflexi 31.06% 38.27%, under reduction, respectively. positively correlated both DISS (p<0.05), indicating may stimulate species associated carbon cycling, thereby enhancing fertility, nutrient absorption, ultimately increased accumulation production components addition, there no significant difference contents between reduced dosage combined solid inoculant (SMI) polyglutamic (PMI), compared 100% fertilizer. Correlation analysis revealed PMI could phosphorus availability through Streptomyces recruitment. conclusion, our findings demonstrated bio-organic can substitute improve microorganisms, This provides theoretical basis productivity reduction.

Язык: Английский

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Microbial Community Structure and Causal Analysis. in Sediments of Shallow Eutrophic Freshwater Lakes. Under Heavy Metal Compound Pollution

Journal of Hazardous Materials, Год журнала: 2025, Номер 487, С. 137135 - 137135

Опубликована: Янв. 7, 2025

Язык: Английский

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Bacillus atrophaeus DX-9 biocontrol against potato common scab involves significant changes in the soil microbiome and metabolome

aBIOTECH, Год журнала: 2025, Номер 6(1), С. 33 - 49

Опубликована: Фев. 18, 2025

Язык: Английский

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Achieving synchronous nitrogen and phosphorus removal by aerobic enrichment of electrotrophic/heterotrophic bacteria and denitrifying polyphosphate-accumulating organisms in repeatedly oxygen-rich microbial fuel cells

Bioresource Technology, Год журнала: 2025, Номер unknown, С. 132297 - 132297

Опубликована: Фев. 1, 2025

Язык: Английский

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Intercropping grapevine with Solanum nigrum enhances their cadmium tolerance through changing rhizosphere soil microbial diversity

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Март 7, 2025

Introduction Cadmium (Cd), a toxic heavy metal, has increasingly impacted vineyard soils and grapevine ( Vitis vinifera ) production in recent years. Intercropping with the hyperaccumulator plant Solanum nigrum emerged as promising strategy to improve soil health increase resilience Cd-contaminated soil. Methods This study investigated effects of intercropping S. (IntVVSN) on enzyme activity microbial community. Results Compared monocultures grapevine, IntVVSN increased activities sucrase, urease, cellulase, decreased amylase, neutral phosphatase. The community showed higher abundances beneficial groups such Acidobacteriota, Actinobacteriota, Chloroflexi. These were involved metal detoxification nutrient cycling, indicating their potential role enhancing Cd tolerance. Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analysis revealed distinct metabolic adaptations under soil, significant upregulation pathways related secondary metabolite synthesis, carbohydrate metabolism, glycan biosynthesis, nucleotide protein processing. changes composition, along enhanced cycling indicated by activities, suggest healthier more resilient environment. This, turn, contributes improved tolerance grapevines. Conclusion highlights phytoremediation intercropping, which promotes sustainable agricultural practices improving growth stress.

Язык: Английский

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Patchy burn severity explains heterogeneous soil viral and prokaryotic responses to fire in a mixed conifer forest

mSystems, Год журнала: 2025, Номер unknown

Опубликована: Май 14, 2025

ABSTRACT Effects of fire on soil viruses and virus–host dynamics are largely unexplored, despite known microbial contributions to biogeochemical processes ecosystem recovery. Here, we assessed how viral prokaryotic communities responded a prescribed burn in mixed conifer forest. We sequenced 91 viral-size fraction metagenomes (viromes) 115 16S rRNA gene amplicon libraries from 120 samples: four samples at five timepoints (two before three after fire) six sites (four treatment, two control). hypothesized that compositional differences would be most significant between burned unburned soils, but instead, plot location best distinguished communities, more than treatment (burned or not), depth (0–3 3–6 cm), timepoint. For both prokaryotes, some resembled controls, while others were significantly different, revealing heterogeneous responses fire. These patterns explained by severity, here defined chemistry. Viral not richness decreased with low viromic DNA yields indicated substantial loss biomass higher severity. The relative abundances Firmicutes, Actinobacteriota, the predicted infect them increased suggesting survival infection these fire-responsive potentially spore-forming taxa. degree severity experienced each patch soil, rather status alone, differed over mere meters same Therefore, our analyses highlight importance high-resolution, paired data explain community IMPORTANCE impact microbiome, particularly understudied warrants investigation, given collected forest assess impacts this disturbance communities. show simple categorical comparisons areas insufficient reveal underlying response patterns. patchy nature (indicated chemistry data) led changes composition high lower indistinguishable those controls. Our results considering highly resolved measurements, even nearby soils fire, order understand burns.

Язык: Английский

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Fertilization alters soil carbon metabolism by affecting the composition of modular microorganisms in paddy soil

Soil Use and Management, Год журнала: 2025, Номер 41(1)

Опубликована: Янв. 1, 2025

Abstract Soil microbes play a significant role in the carbon cycle. However, our understanding of how soil respond to different substrates is still limited. Here, we investigated ecological mechanisms behind overall metabolism differences under fertilizations paddy ecosystem. Four fertilization treatments, no fertilizers (CK), mineral nitrogen, phosphorus and potassium (NPK), plus organic manures (NPKM) straw return (NPKS) were set up. Our results indicated that drove shifts microbial community structure, with reduction abundance Actinobacteriota an increase Chloroflexi . NPKS CK exhibited higher utilization capacity across various sources, particularly metabolic activity for carbohydrates than NPK NPKM treatments. The weighted gene co‐expression network analysis (WGCNA) was used evaluate correlation between modules WGCNA metabolism. We found are important contributors variations It key species module affecting carbohydrate C0119 , which belongs Ktedonobacteria suggest could mediate core bacterial affect finally function.

Язык: Английский

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Microbiome of the Soil and Rhizosphere of the Halophyte Spergularia marina (L.) Griseb in the Saline Sites of Lake Kurgi, the South Urals: Metagenomic Analysis

Microbiology Research, Год журнала: 2025, Номер 16(3), С. 64 - 64

Опубликована: Март 10, 2025

The study of the metagenomes bacterial communities in saline areas is relevant connection with global salinization agricultural lands. aim this was to investigate biodiversity and structure rhizobacterial associated halophyte S. marina from low moderate sulfate–chloride salinity habitats. community bulk rhizosphere soil analyzed using high-throughput sequencing V1–V9 region 16S rRNA by Oxford Nanopore Technologies. Alpha beta diversity indices were calculated. A total 55 phyla 309 genera bacteria identified, among which Proteobacteria Bacteroidetes dominated. occurrence Planctomycetes, Verrucomicrobia, Acidobacteria higher than soil. Bacterial alpha decreased increasing salinity, while it increased rhizosphere. proportion halotolerant Flavobacterium Alteromonas significantly grew both In addition, rhizosphere, percentage Comamonas, Methylibium, Lysobacter, Planctomyces, Sphingomonas, Stenotrophomonas, Lewinella increased. Among them, several included plant growth promoting rhizobacteria (PGPR). more soil, Bacillus, Salinimicrobium, Marinobacter, Clostridium, Euzebya, KSA1, Halorhodospira also compared site. Thus, changed taxonomic

Язык: Английский

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Spatial Heterogeneity of the Microbial Community in the Surface Sediments in the Okinawa Trough

Journal of Marine Science and Engineering, Год журнала: 2025, Номер 13(4), С. 653 - 653

Опубликована: Март 25, 2025

The Okinawa Trough (OT) has been a focus of scientific research for many years due to the presence vibrant hydrothermal and cold seep activity within its narrow basin. However, spatial distribution environmental drivers microbial communities in OT sediments remain poorly understood. present study aims address this knowledge gap by investigating diversity abundance at ten different sampling sites transitional zone between vents seeps OT. community two (G08 G09) close proximity showed high degree similarity. lower bacterial archaeal abundances were found these sites. groups, classified as Hydrothermarchaeota Thermoplasmata, comparatively higher relative In addition, ammonia-oxidizing archaea (AOA), from family Nitrosopumilaceae, have surface G03, G04, G05, G06, G07. This result suggests that ammonia oxidation may be actively occurring areas. Furthermore, Methylomirabilaceae, which are responsible methane coupled with nitrite reduction, dominated three (G07, G08, G09), implying N-DAMO play an important role mitigating emissions. Using FAPROTAX database, we predicted prokaryotic functional groups involved methyl-reducing methanogenesis hydrogenotrophic most abundant G08 G09. At G01 G02, such hydrocarbon degradation, methanotrophy, methanol oxidation, denitrification, sulfate respiration, sulfur more abundant. Nitrogen content is factor determining sediments. These results expand our

Язык: Английский

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Performances and mechanisms for improving the river water quality by a simple integration of mussels and immobilized microorganisms

Environmental Technology, Год журнала: 2025, Номер unknown, С. 1 - 12

Опубликована: Апрель 2, 2025

The complexity of ecological remediation technology restricts its long-term application in engineering practice. In this study, a simple top-down mussels-bacteria (TDMB) integrated system used for river water quality improvement was constructed, and the operation performances accompanied by corresponding mechanisms were investigated. results showed that during 161 days operation, average removal efficiency achieved 75.45% COD, 79.41% ammonia nitrogen 70% total (TN), while contribution proportions mussels immobilized microorganisms TN 28.07% 69.77%, respectively. High-throughput sequencing revealed dominant immobilization norank_SC-I-84 (10.52%), norank_PHOS-HE36 (9.58%), norank_Anaerolineaceae (3.04%), Nitrospira (2.64%), norank_Gemmatimonadaceae (2.36%) norank_Pirellulaceae (2.113%), all which involved removal. This study provided promising strategy gained insight into polluted rivers.

Язык: Английский

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