mSystems, Год журнала: 2021, Номер 6(3)
Опубликована: Май 10, 2021
Drosophila is an important model for microbiome research partly because of the low complexity its mostly culturable gut microbiota. Our current understanding how interacts with microbes and these interactions influence host traits derives almost entirely from empirical studies that focus on individual microbial taxa or classes metabolites.
Язык: Английский
Microbiome, Год журнала: 2020, Номер 8(1)
Опубликована: Март 16, 2020
The insect gut microbiota has been shown to contribute the host's digestion, detoxification, development, pathogen resistance, and physiology. However, there is poor information about ranking of these roles. Most results were obtained with cultivable bacteria, whereas bacterial physiology may be different between free-living midgut-colonizing bacteria. In this study, we provided both proteomic genomic evidence on roles bacteria by investigating anal droplets from a weevil, Cryptorhynchus lapathi.The lumen showed qualitatively quantitatively subsets communities. 16S rRNA sequencing that dominated Proteobacteria Bacteroidetes, are Proteobacteria. From droplets, enzymes involved in 31 basic belong 7 super identified Q-TOF MS. cooperation weevil its was determined reconstructing community pathway maps, which defined study. A score used rank data indicate most dominant role amino acid biosynthesis, followed protein energy metabolism, vitamin lipid plant secondary metabolite (PSM) degradation, carbohydrate while order PSM digestion. PCA form functional groups point view either or role, MFA variations among addition, data, analyzed HMFA method individual species, presented.The essential nutrient provisioning, digestion detoxification. plays pioneering diet mainly digests macromolecules into smaller molecules then digested
Язык: Английский
Процитировано
241
Nature Reviews Microbiology, Год журнала: 2019, Номер 17(12), С. 764 - 775
Опубликована: Авг. 15, 2019
Язык: Английский
Процитировано
212
Nature Communications, Год журнала: 2023, Номер 14(1)
Опубликована: Март 21, 2023
Abstract The gut is continuously invaded by diverse bacteria from the diet and environment, yet microbiome composition relatively stable over time for host species ranging mammals to insects, suggesting host-specific factors may selectively maintain key of bacteria. To investigate specificity, we used gnotobiotic Drosophila , microbial pulse-chase protocols, microscopy stability different strains in fly gut. We show that a host-constructed physical niche foregut binds with strain-level stabilizing their colonization. Primary colonizers saturate exclude secondary same strain, but initial colonization Lactobacillus physically remodels through production glycan-rich secretion favor unrelated commensals Acetobacter genus. Our results provide mechanistic framework understanding establishment multi-species intestinal microbiome.
Язык: Английский
Процитировано
49
Advanced Gut & Microbiome Research, Год журнала: 2025, Номер 2025(1)
Опубликована: Янв. 1, 2025
The microbiome, a diverse ecosystem of microorganisms within the human body, interacts intricately with immune system to maintain health and prevent disease. This dynamic partnership influences development, inflammation regulation, metabolic health, neurological well‐being, while disruptions, termed dysbiosis, have been linked autoimmune disorders, chronic diseases, mental issues. review highlights microbiome’s role in educating system, modulating responses, supporting physical barriers, addressing key research advances, such as microbial metabolites regulation impact on cancer immunotherapy. Emerging technologies like multiomics machine learning propelled microbiome research, unlocking potential for personalized medicine microbiome‐targeted therapies. Despite progress, challenges remain understanding diversity, individual variability, causation–correlation microbiome–immune interactions. Future directions include precision medicine, engineering, public interventions, interdisciplinary collaborations, positioning symbiosis transformative frontier healthcare innovation.
Язык: Английский
Процитировано
3
Frontiers in Physiology, Год журнала: 2020, Номер 11
Опубликована: Май 7, 2020
Lipids are the primary storage molecules and an essential source of energy in insects during reproduction, prolonged periods flight, starvation, diapause. The coordination center for insect lipid metabolism is fat body, which analogous to vertebrate adipose tissue liver. body primarily composed adipocytes, accumulate triacylglycerols intracellular droplets. Genomics proteomics, together with functional analyses, such as RNA interference CRISPR/Cas9-targeted genome editing, identified various genes involved elucidated their functions. However, endocrine control metabolism, particular roles peptide hormones lipogenesis lipolysis relatively less-known topics. In current review, neuropeptides that directly or indirectly affect introduced. lipolytic lipogenic adipokinetic hormone brain insulin-like peptides (ILP2, ILP3, ILP5). Other neuropeptides, insulin-growth factor ILP6, neuropeptide F, allatostatin-A, corazonin, leucokinin, tachykinins limostatin, might stimulate lipolysis, while diapause hormone-pheromone biosynthesis activating neuropeptide, short CCHamide-2, cytokines Unpaired 1 2 induce lipogenesis. Most these interact one another, but mostly insulin signaling, therefore indirectly. Peptide also diapause, infections immunity; highlighted. review concludes a discussion potential metabolism-related pest management.
Язык: Английский
Процитировано
118
Nature Communications, Год журнала: 2020, Номер 11(1)
Опубликована: Авг. 25, 2020
Abstract The impact of commensal bacteria on the host arises from complex microbial-diet-host interactions. Mapping metabolic interactions in gut microbial communities is therefore key to understand how microbiome influences host. Here we use an interdisciplinary approach including isotope-resolved metabolomics show that Drosophila melanogaster, Acetobacter pomorum ( Ap ) and Lactobacillus plantarum Lp a syntrophic relationship established overcome detrimental diets identify as bacterium altering host’s feeding decisions. Specifically, uses lactate produced by supply amino acids are essential , allowing it grow imbalanced diets. Lactate also necessary sufficient for alter fly’s protein appetite. Our data bacterial become resilient These ensure constant flow metabolites used reproduction behaviour.
Язык: Английский
Процитировано
118
PLoS Pathogens, Год журнала: 2020, Номер 16(4), С. e1008398 - e1008398
Опубликована: Апрель 23, 2020
Язык: Английский
Процитировано
97
Current Opinion in Insect Science, Год журнала: 2020, Номер 39, С. 84 - 90
Опубликована: Март 20, 2020
Drosophila melanogaster harbors a simple gut microbial community, or microbiome, that regulates several facets of its physiology. As result, the host employs multiple mechanisms maintaining control over microbiome in an effort to promote overall organismal homeostasis. Perturbations balance between and can result states instability disease, making maintenance homeostasis fundamental physiologic aspect D. biology. While interactions microbes their hosts be direct, particularly context immunity renewal, effects resulting from indirect interactions, such as those microbiota members, equally important. This review highlights major ways, which homeostasis, consequences disruptions different mechanisms, by interacts with host.
Язык: Английский
Процитировано
77
iScience, Год журнала: 2020, Номер 23(6), С. 101232 - 101232
Опубликована: Июнь 1, 2020
The gut microbiota shapes animal growth trajectory in stressful nutritional environments, but the molecular mechanisms behind such physiological benefits remain poorly understood. is mostly composed of bacteria, which construct metabolic networks among themselves and with host. Until now, how activities contribute to host juvenile remains unknown. Here, using Drosophila as a model, we report that two its major bacterial partners, Lactobacillus plantarum Acetobacter pomorum, engage beneficial dialogue boosts despite stress. We pinpoint lactate, produced by L. plantarum, utilized A. pomorum an additional carbon source, provides essential amino acids vitamins plantarum. Such cross-feeding provisions set anabolic metabolites host, may foster systemic poor nutrition.
Язык: Английский
Процитировано
75
Gut Microbes, Год журнала: 2023, Номер 15(1)
Опубликована: Май 2, 2023
The gut epithelia of virtually all animals harbor complex microbial communities that play an important role in maintaining immune and cellular homeostasis. Gut microbiota have evolutionarily adapted to the host environment, serving as key regulators intestinal stem cells promote a healthy barrier modulate epithelial self-renewal. Disruption these populations has been associated with inflammatory disorders or cancerous lesions intestine. However, molecular mechanisms controlling gut-microbe interactions are only partially understood due high diversity biologically dynamic nature microorganisms. This article reviews current knowledge on Drosophila its signaling pathways crucial for induction distinct homeostatic responses. Thanks genetic tractability cultivable simple microbiota, this association model offers new efficient tools investigating crosstalk between while providing framework better understanding ecological evolutionary roles microbiome.
Язык: Английский
Процитировано
28