Microcystis pangenome reveals cryptic diversity within and across morphospecies

Science Advances, Год журнала: 2023, Номер 9(2)

Опубликована: Янв. 13, 2023

Microcystis, a common harmful algal bloom (HAB) taxon, threatens water supplies and human health, yet species delimitation is contentious in this leading to challenges research management of threat. Historical morphology-based classifications recognize multiple morphospecies, most with variable diverse ecologies, while DNA sequence-based indicate single ecotypes. To better delimit Microcystis species, we conducted pangenome analysis 122 genomes. Core- non-core gene phylogenetic analyses placed 113 genomes into 23 monophyletic clusters containing at least two Overall, genome-related indices revealed that contains 16 putative genospecies. Fifteen genospecies included one aeruginosa 10 or more morphospecies. This classification system will enable consistent taxonomic identification thereby aid resolving some the complexities controversies have long characterized eco-evolutionary important HAB taxon.

Язык: Английский

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Harmful Algal Blooms in Eutrophic Marine Environments: Causes, Monitoring, and Treatment

Water, Год журнала: 2024, Номер 16(17), С. 2525 - 2525

Опубликована: Сен. 5, 2024

Marine eutrophication, primarily driven by nutrient over input from agricultural runoff, wastewater discharge, and atmospheric deposition, leads to harmful algal blooms (HABs) that pose a severe threat marine ecosystems. This review explores the causes, monitoring methods, control strategies for eutrophication in environments. Monitoring techniques include remote sensing, automated situ sensors, modeling, forecasting, metagenomics. Remote sensing provides large-scale temporal spatial data, while sensors offer real-time, high-resolution monitoring. Modeling forecasting use historical data environmental variables predict blooms, metagenomics insights into microbial community dynamics. Control treatments encompass physical, chemical, biological treatments, as well advanced technologies like nanotechnology, electrocoagulation, ultrasonic treatment. Physical such aeration mixing, are effective but costly energy-intensive. Chemical including phosphorus precipitation, quickly reduce levels may have ecological side effects. Biological biomanipulation bioaugmentation, sustainable require careful management of interactions. Advanced innovative solutions with varying costs sustainability profiles. Comparing these methods highlights trade-offs between efficacy, cost, impact, emphasizing need integrated approaches tailored specific conditions. underscores importance combining mitigate adverse effects on

Язык: Английский

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Two decades of bacterial ecology and evolution in a freshwater lake

Nature Microbiology, Год журнала: 2025, Номер 10(1), С. 246 - 257

Опубликована: Янв. 3, 2025

Язык: Английский

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Patterns in sources and forms of nitrogen in a large eutrophic lake during a cyanobacterial harmful algal bloom

Limnology and Oceanography, Год журнала: 2023, Номер 68(4), С. 803 - 815

Опубликована: Фев. 2, 2023

Abstract Western Lake Erie experiences an annual, toxic cyanobacterial harmful algal bloom (cyanoHAB), primarily caused by excess anthropogenic inputs of nitrogen (N) and phosphorous (P) . Because the non‐N fixing cyanobacteria species Microcystis dominates these blooms, N availability is hypothesized to play a central role in cyanoHAB progression, as well production N‐rich toxin microcystin. Many previous studies focused on nitrate because it most abundant substrate during initiation. However, recent work implicated reduced substrates like ammonium dissolved organic (DON) promoting greater biomass longevity. To examine relative importance oxidized phytoplankton different stages, we measured concentrations natural abundance δ 15 isotope values throughout entirety 2020 Erie. The results provide first data DON dynamics composition Erie, suggest that phytoplankton, including , likely relied regenerated from pool later stages. In addition, stable confirm delivered via Maumee River growth production.

Язык: Английский

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Microcystin congeners in Lake Erie follow the seasonal pattern of nitrogen availability

Harmful Algae, Год журнала: 2023, Номер 127, С. 102466 - 102466

Опубликована: Июнь 2, 2023

Cyanobacteria harmful algal blooms produce many toxic secondary metabolites called cyanotoxins. The most studied group of cyanotoxins are microcystins (MC), with over 300 congeners reported. MC-LR is the congener because its abundance and toxicity. Recent toxicology studies suggest that more hydrophobic MC such as MC-LA, MC-LF, MC-LW may be less abundant but up to seven times than MC-LR, whereas, MC-RR's toxicity only one-fifth MC-LR. Hence, understanding environmental stressors change profile critical assessing negative impact on human health. A two-year field experimental study investigated seasonal spatial changes profiles in western basin Lake Erie. Both showed nitrogen enrichment favored production nitrogen-rich MC-RR (C49H75N13O12). depletion low-nitrogen MC-LA (C46H67N7O12). (a medium N level, C49H75N10O12) accounted for ∼30% 50% total concentration was stable across concentrations. Using relative concentrations each congener, both LC-MS/MS ELISA overestimated early bloom (July) underestimated it late (September). On 24 July 2019, highly MC-LF were detected at nearshore stations exceeding drinking water standards. This demonstrated toxic, high dominated under nitrogen-replete conditions season, whereas nitrogen-limited later season.

Язык: Английский

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The biological functions of microcystins

Water Research, Год журнала: 2024, Номер 262, С. 122119 - 122119

Опубликована: Июль 18, 2024

Язык: Английский

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Routine monitoring of western Lake Erie to track water quality changes associated with cyanobacterial harmful algal blooms

Earth system science data, Год журнала: 2023, Номер 15(8), С. 3853 - 3868

Опубликована: Авг. 25, 2023

Abstract. The western basin of Lake Erie has a history recurrent cyanobacterial harmful algal blooms (HABs) despite decades efforts by the United States and Canada to limit phosphorus loading, major driver blooms. In response, National Oceanic Atmospheric Administration (NOAA) Great Lakes Environmental Research Laboratory (GLERL) Cooperative Institute for (CIGLR) created an annual sampling program detect, monitor, assess, predict HABs in (WLE). Here we describe data collected from this monitoring 2012 2021. This dataset includes observations on physicochemical properties, nutrient fractions, phytoplankton pigments, microcystins, optical properties Erie. is particularly relevant creating models, verifying calibrating remote sensing algorithms, informing experimental research further understand water quality dynamics that influence internationally significant body freshwater. can be freely accessed NOAA Centers Information (NCEI) at https://doi.org/10.25921/11da-3x54 (Cooperative Research, University Michigan Laboratory, 2019).

Язык: Английский

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Spatio-temporal connectivity of a toxic cyanobacterial community and its associated microbiome along a freshwater-marine continuum

Harmful Algae, Год журнала: 2024, Номер 134, С. 102627 - 102627

Опубликована: Апрель 1, 2024

Due to climate changes and eutrophication, blooms of predominantly toxic freshwater cyanobacteria are intensifying likely colonize estuaries, thus impacting benthic organisms shellfish farming representing a major ecological, health economic risk. In the natural environment, Microcystis form large mucilaginous colonies that influence development both cyanobacterial embedded bacterial communities. However, little is known about fate by salinity increase. this study, we monitored dominated bloom its microbiome along French freshwater-marine gradient at different phases bloom. We demonstrated in genotypic composition, production specific metabolites (toxins compatible solutes) heterotrophic bacteria structure response particular M. aeruginosa wesenbergii survived salinities up 20. Based on microcystin gene abundance, became more during their estuarine transfer but with no selection variants. An increase solutes occurred continuum extensive trehalose betaine accumulations. Salinity structured most community, an increased richness diversity continuum. A core mucilage-associated attached fraction was highly abundant suggesting strong interaction between protecting role mucilage against osmotic shock. These results underline need better determine interactions as key widespread success adaptation various environmental conditions.

Язык: Английский

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Transcriptionally active nitrogen fixation and biosynthesis of diverse secondary metabolites by Dolichospermum and Aphanizomenon-like Cyanobacteria in western Lake Erie Microcystis blooms

Harmful Algae, Год журнала: 2023, Номер 124, С. 102408 - 102408

Опубликована: Фев. 20, 2023

Язык: Английский

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Lake Erie field trials to advance autonomous monitoring of cyanobacterial harmful algal blooms

Frontiers in Marine Science, Год журнала: 2022, Номер 9

Опубликована: Окт. 6, 2022

Biomolecular analyses are used to investigate the dynamics of cyanobacterial harmful algal blooms (cyanoHABs), with samples collected during monitoring often analyzed by qPCR and sometimes amplicon metagenomic sequencing. However, cyanoHAB research programs face operational constraints due reliance on human resources for sample collections. To address this impediment, a third-generation Environmental Sample Processor (3G ESP) integrated long-range autonomous underwater vehicle (LRAUV) was tested seasonal Microcystis in western Lake Erie (WLE) 2018 2019. The LRAUV-3G ESP successfully performed flexible, sampling across wide range conditions, results indicated equivalency between manual methods. No significant differences were found collection handling methods 12 parameters tested. Analyzed included concentrations total cyanobacteria microcystin toxin gene via qPCR; relative abundances bacterial sequence variants (ASVs) from 16S rRNA sequencing; community diversity measures both provided additional capacity revealed field seasons taxa gene. Metagenomic analysis multiple genes corroborated use mcyE as proxy genomic potential WLE cyanoHABs produce microcystin. Overall, study provides support ‘omics capability help expand spatial temporal coverage operations.

Язык: Английский

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