A subcellular biochemical model for T6SS dynamics reveals winning competitive strategies DOI Creative Commons
Yuexia Luna Lin, Stephanie Smith, Eva Kanso

и другие.

PNAS Nexus, Год журнала: 2023, Номер 2(7)

Опубликована: Июнь 13, 2023

Abstract The type VI secretion system (T6SS) is a broadly distributed interbacterial weapon that can be used to eliminate competing bacterial populations. Although unarmed target populations are typically study T6SS function in vitro, bacteria most likely encounter other T6SS-armed competitors nature. However, the connection between subcellular details of and outcomes such mutually lethal battles not well understood. Here, we incorporate biological data derived from natural Vibrio fischeri light organ symbionts build biochemical model for at single-cell level, which then integrate into an agent-based (ABM). Using ABM, isolate experiment with strain-specific physiological differences ways possible samples identify winning strategies Through vitro experiments, discover exist activation speed. ABM simulations corroborate faster dominant determining survival during competition. Once fully activated, energy required creates tipping point where increased building firing becomes too costly advantageous. simulations, threshold this transition occurs parameter space. We also find competitive depend on geometry battlefield: cells survive edges range expansion unlimited territory claimed. Alternatively, competitions within confined space, much like crypts V. compete, result rapid elimination population.

Язык: Английский

A lasting symbiosis: how Vibrio fischeri finds a squid partner and persists within its natural host DOI
Karen L. Visick, Eric V. Stabb, Edward G. Ruby

и другие.

Nature Reviews Microbiology, Год журнала: 2021, Номер 19(10), С. 654 - 665

Опубликована: Июнь 4, 2021

Язык: Английский

Процитировано

98
Stony coral tissue loss disease: a review of emergence, impacts, etiology, diagnostics, and intervention DOI Creative Commons
Erin Papke, Ashley M. Carreiro,

Caroline E. Dennison

и другие.

Frontiers in Marine Science, Год журнала: 2024, Номер 10

Опубликована: Янв. 25, 2024

Stony coral tissue loss disease (SCTLD) is destructive and poses a significant threat to Caribbean reef ecosystems. Characterized by the acute of tissue, SCTLD has impacted over 22 stony species across region, leading visible declines in health. Based on duration, lethality, host range, spread this disease, considered most devastating outbreak ever recorded. Researchers are actively investigating cause transmission SCTLD, but exact mechanisms, triggers, etiological agent(s) remain elusive. If left unchecked, could have profound implications for health resilience reefs worldwide. To summarize what known about identify potential knowledge gaps, review provides holistic overview research, including susceptibility, transmission, ecological impacts, etiology, diagnostic tools, defense treatments. Additionally, future research avenues highlighted, which also relevant other diseases. As continues spread, collaborative efforts necessary develop effective strategies mitigating its impacts critical These need include researchers from diverse backgrounds underrepresented groups provide additional perspectives that requires creative urgent solutions.

Язык: Английский

Процитировано

14
Targeted Depletion of Bacteria from Mixed Populations by Programmable Adhesion with Antagonistic Competitor Cells DOI Creative Commons
See‐Yeun Ting, Esteban Martínez‐García, Shuo Huang

и другие.

Cell Host & Microbe, Год журнала: 2020, Номер 28(2), С. 313 - 321.e6

Опубликована: Май 28, 2020

Язык: Английский

Процитировано

69
Lighting the way: how the Vibrio fischeri model microbe reveals the complexity of Earth’s “simplest” life forms DOI Creative Commons
Alecia N. Septer, Karen L. Visick

Journal of Bacteriology, Год журнала: 2024, Номер 206(5)

Опубликована: Май 2, 2024

ABSTRACT Vibrio ( Aliivibrio ) fischeri ’s initial rise to fame derived from its alluring production of blue-green light. Subsequent studies probe the mechanisms underlying this bioluminescence helped field discover phenomenon now known as quorum sensing. Orthologs quorum-sensing regulators (i.e., LuxR and LuxI) originally identified in V. were subsequently uncovered a plethora bacterial species, analogous pathways found yet others. Over past three decades, study microbe has greatly expanded unique role exclusive symbiont light organ Hawaiian bobtail squid, Euprymna scolopes . Buoyed by optically amenable host persistent insightful researchers who have applied novel cross-disciplinary approaches, developed into robust model for microbe-host associations. It contributed our understanding how bacteria experience respond specific, often fluxing environmental conditions which impact development their host. also deepened numerous microbial processes such motility chemotaxis, biofilm formation dispersal, competition, relevance specific genes context colonizing an animal Parallels these between studied pathogens are readily apparent, demonstrating functional conservation across diverse associations permitting reinterpretation “pathogenesis.” Collectively, advances built foundation microbiome positioned continue expand frontiers world inside animals.

Язык: Английский

Процитировано

7
Vibrio fischeri : a model for host-associated biofilm formation DOI

Brittany L. Fung,

Jeremy J. Esin, Karen L. Visick

и другие.

Journal of Bacteriology, Год журнала: 2024, Номер 206(2)

Опубликована: Янв. 25, 2024

ABSTRACT Multicellular communities of adherent bacteria known as biofilms are often detrimental in the context a human host, making it important to study their formation and dispersal, especially animal models. One such model is symbiosis between squid Euprymna scolopes bacterium Vibrio fischeri . Juvenile hatch aposymbiotically selectively acquire symbiont from natural seawater containing diverse environmental microbes. Successful pairing facilitated by ciliary movements that direct quiet zones on surface squid’s symbiotic light organ where V. forms small aggregate or biofilm. Subsequently, disperse enter organ, ultimately reaching colonizing deep crypt spaces. Although transient, critical for optimal colonization tightly controlled. In vitro studies have identified variety polysaccharides proteins comprise extracellular matrix. Some most well-characterized matrix factors include polysaccharide (SYP), cellulose polysaccharide, LapV adhesin. this review, we discuss these components, regulation, other less understood biofilm contributors. We also highlight what currently about dispersal aggregates host cues may promote it. Finally, briefly describe discoveries gleaned isolates. By unraveling complexities involved ’s control over begin understand how environment triggers transient unique relationship.

Язык: Английский

Процитировано

6
Host Adaptation Predisposes Pseudomonas aeruginosa to Type VI Secretion System-Mediated Predation by the Burkholderia cepacia Complex DOI Creative Commons
Andrew I. Perault, Courtney E. Chandler, David A. Rasko

и другие.

Cell Host & Microbe, Год журнала: 2020, Номер 28(4), С. 534 - 547.e3

Опубликована: Авг. 4, 2020

Язык: Английский

Процитировано

45
A Pseudomonas aeruginosa Antimicrobial Affects the Biogeography but Not Fitness of Staphylococcus aureus during Coculture DOI
Juan P. Barraza, Marvin Whiteley

mBio, Год журнала: 2021, Номер 12(2)

Опубликована: Март 27, 2021

Pseudomonas aeruginosa and Staphylococcus aureus are two of the most common coinfecting bacteria in human infections, including cystic fibrosis (CF) lung. There is emerging evidence that coinfection with these microbes enhances disease severity antimicrobial tolerance through direct interactions. However, one challenges to studying microbial interactions relevant infection lack experimental models versatility investigate complex interaction dynamics while maintaining biological relevance. Here, we developed a model based on an vitro medium mimics CF lung secretions (synthetic sputum [SCFM2]) allows time-resolved assessment fitness community spatial structure at micrometer scale. Our results reveal P. S. coexist as spatially structured communities SCFM2 under static growth conditions, enriched distance 3.5 μm from Multispecies aggregates were rare, aggregate (biofilm) sizes resembled those sputum. Elimination aeruginosa's ability produce antistaphylococcal small molecule HQNO (2-heptyl-4-hydroxyquinoline N-oxide) had no effect bacterial but altered by increasing 7.6 μm. Lastly, show coculture sensitizes killing antibiotic tobramycin compared monoculture despite enhancing during coculture. findings powerful for alters biogeography susceptibility without affecting fitness.IMPORTANCE Many infections result action multispecies communities. Within communities, have been proposed directly interact via physical chemical means, resulting increased tolerance. One robust, infection-relevant systems study time micrometer-scale precision. versatile between aureus, commonly infections. Using this along high-resolution, single-cell microscopy, showed form scale, controlled part production In addition, provide aminoglycoside only

Язык: Английский

Процитировано

33
The Depletion Mechanism Actuates Bacterial Aggregation by Exopolysaccharides and Determines Species Distribution & Composition in Bacterial Aggregates DOI Creative Commons
Patrick R. Secor, Lia A. Michaels, DeAnna C. Bublitz

и другие.

Frontiers in Cellular and Infection Microbiology, Год журнала: 2022, Номер 12

Опубликована: Июнь 16, 2022

Bacteria in natural environments and infections are often found cell aggregates suspended polymer-rich solutions, aggregation can promote bacterial survival stress resistance. One mechanism, called depletion aggregation, is driven by physical forces between bacteria high concentrations of polymers the environment rather than activity per se. As such, aggregated mechanism will disperse when polymer fall unless other adhesion mechanisms supervene. Here we investigated whether actuate aggregating effects Pseudomonas aeruginosa exopolysaccharides for (i.e. not surface attached) bacteria, how affects inter-species interactions. We that cells overexpressing Pel Psl remained after short periods whereas wild-type mucoid P. did not. In co-culture, had contrasting on aeruginosa's interactions with coccus- rod-shaped bacteria. Depletion caused S. aureus (cocci) (rods) to segregate from each resist secreted antimicrobial factors resulting species co-existence. contrast, Burkholderia sp. (both rods) intermix, enhancing type VI secretion inhibition aeruginosa, leading dominance. These results show addition being a primary cause suspensions, inherent some self-produced determine distribution composition communities.

Язык: Английский

Процитировано

27
Community composition and the environment modulate the population dynamics of type VI secretion in human gut bacteria DOI

Sophie Robitaille,

Emilia L. Simmons, Adrian J. Verster

и другие.

Nature Ecology & Evolution, Год журнала: 2023, Номер 7(12), С. 2092 - 2107

Опубликована: Окт. 26, 2023

Язык: Английский

Процитировано

16
Evaluation of Sample Preservation and Storage Methods for Metaproteomics Analysis of Intestinal Microbiomes DOI
Angie L. Mordant, Manuel Kleiner

Microbiology Spectrum, Год журнала: 2021, Номер 9(3)

Опубликована: Дек. 15, 2021

A critical step in studies of the intestinal microbiome using meta-omics approaches is preservation samples before analysis. Preservation essential for that measure gene expression, such as metaproteomics, which used to identify and quantify proteins microbiomes. Intestinal are typically stored by flash-freezing storage at -80°C, but some experimental setups do not allow immediate freezing samples. In this study, we evaluated methods preserve fecal metaproteomics analyses when possible. We collected from C57BL/6 mice them 1 4 weeks following methods: liquid nitrogen, immersion RNAlater, 95% ethanol, a RNAlater-like buffer, combinations these methods. After storage, extracted protein prepared peptides chromatography with tandem mass spectrometry (LC-MS/MS) analysis proteins. All produced highly similar metaproteomes, except ethanol-preserved were distinct all other terms identifications abundance profiles. Flash-freezing RNAlater (or treatments) metaproteomes differed only slightly, less than 0.7% identified differing abundance. contrast, ethanol resulted an average 9.5% between treatments. Our results suggest room temperature or solution performs well analyses. IMPORTANCE Metaproteomics powerful tool study microbiome. By identifying quantifying large number microbial, dietary, host samples, provides direct evidence activities functions microbial community members. workflows preserving because profiles susceptible fast changes response environmental conditions (air exposure, changes, etc.). This effects different treatments on contrast prior work analyses, ensured steps sample identical so differences could be attributed method.

Язык: Английский

Процитировано

28