Alterations in Gut Microbiota by Statin Therapy and Possible Intermediate Effects on Hyperglycemia and Hyperlipidemia

Frontiers in Microbiology, Год журнала: 2019, Номер 10

Опубликована: Сен. 4, 2019

Dysbiosis of the gut microbiota is a contributing factor for obesity-related metabolic diseases such as hyperglycemia and hyperlipidemia. Pharmacotherapy involves modulation microbiota, which suggested to be potential therapeutic target. In this study, by statins (cholesterol-lowering drugs: atorvastatin rosuvastatin) was investigated in an aged mouse model high-fat diet-induced obesity, association between immune responses described. Atorvastatin rosuvastatin significantly increased abundance genera Bacteroides, Butyricimonas, Mucispirillum. Moreover, these correlated with inflammatory response, including levels IL-1β TGFβ1 ileum. addition, oral fecal transplantation material collected from rosuvastatin-treated groups improved hyperglycemia. From results, effect on improvements could explained altered microbiota. Our findings suggest that has important role actions drugs.

Язык: Английский

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A specific gene-microbe interaction drives the development of Crohn’s disease–like colitis in mice

Science Immunology, Год журнала: 2019, Номер 4(34)

Опубликована: Апрель 12, 2019

Bacterial dysbiosis is associated with Crohn's disease (CD), a chronic intestinal inflammatory disorder thought to result from an abnormal immune response against bacteria in genetically susceptible individuals. However, it unclear whether cause or consequence of inflammation and overall specific trigger the disease. Here, we show that combined deficiency NOD2 phagocyte NADPH oxidase, two CD susceptibility genes, triggers early-onset spontaneous TH1-type mice pathological hallmarks CD. Disease was induced by Mucispirillum schaedleri, Gram-negative mucus-dwelling anaerobe. CYBB deficiencies led marked accumulation Mucispirillum, which impaired neutrophil recruitment killing bacterium luminal neutrophils. Maternal immunoglobulins protected mutant during breastfeeding. Our results indicate microbe CD-like presence clearance innate immunity.

Язык: Английский

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Extrathymically Generated Regulatory T Cells Establish a Niche for Intestinal Border-Dwelling Bacteria and Affect Physiologic Metabolite Balance

Immunity, Год журнала: 2018, Номер 48(6), С. 1245 - 1257.e9

Опубликована: Май 29, 2018

Язык: Английский

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Repertoire of Human Breast and Milk Microbiota: A Systematic Review

Future Microbiology, Год журнала: 2019, Номер 14(7), С. 623 - 641

Опубликована: Апрель 26, 2019

Breastfeeding is a major determinant of human health. Breast milk not sterile and ecological large-scale sequencing methods have revealed an unsuspected microbial diversity that plays important role. However, microbiological analysis at the species level has been neglected while it prerequisite before understanding which microbe associated with symbiosis or dysbiosis, health disease. We review currently known bacterial repertoire from breast microbiota using semiautomated strategy. Total 242 articles 38 countries, 11,124 women 15,489 samples were included. 820 identified mainly composed Proteobacteria Firmicutes. report variations according to analytical method (culture molecular method), anatomical site (breast, colostrum milk) infectious status (healthy control, mastitis, abscess, neonatal infection). In addition, we compared other repertoires. Finally, discuss its putative origin role in

Язык: Английский

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Chemotherapy-induced neuroinflammation is associated with disrupted colonic and bacterial homeostasis in female mice

Scientific Reports, Год журнала: 2019, Номер 9(1)

Опубликована: Ноя. 11, 2019

Abstract Chemotherapy treatment negatively affects the nervous and immune systems alters gastrointestinal function microbial composition. Outside of cancer field, alterations in commensal bacteria have been implicated behavioral deficits; however, extent to which intestinal changes are related chemotherapy-associated comorbidities is not yet known. Thus, this study identified concurrent behavior, central peripheral activation, colon histology, bacterial community structure mice treated with paclitaxel chemotherapy. In paclitaxel-treated mice, increased fatigue decreased cognitive performance occurred parallel reduced microglia immunoreactivity, circulating chemokine expression (CXCL1), as well transient increases pro-inflammatory cytokine/chemokine ( Il-1β, Tnfα, Il-6 , Cxcl1 ) gene brain. Furthermore, had altered colonic composition crypt depth. Relative abundances multiple taxa were associated paclitaxel-induced mass, spleen activation. Although was directly available brain or measures, structural differences tissue strongly activation dentate gyrus prefrontal cortex. These data indicate that chemotherapeutic concurrently gut microbiome, integrity, female thus identifying a novel relationship between integrity responses often assessed studies brain-gut-microbiota axis.

Язык: Английский

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Quercetin improves gut dysbiosis in antibiotic-treated mice

Food & Function, Год журнала: 2020, Номер 11(9), С. 8003 - 8013

Опубликована: Янв. 1, 2020

Diversity and activity of gut microbiota residing in humans animals are significantly influenced by the diet. A quercetin containing diet is effective recovering mice after antibiotic treatment may act as a prebiotic combating dysbiosis.

Язык: Английский

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D-methionine alleviates cisplatin-induced mucositis by restoring the gut microbiota structure and improving intestinal inflammation

Therapeutic Advances in Medical Oncology, Год журнала: 2019, Номер 11

Опубликована: Янв. 1, 2019

There are close links between chemotherapy-induced intestinal mucositis and microbiota dysbiosis. Previous studies indicated that D-methionine was an excellent candidate for a chemopreventive agent. Here, we investigated the effects of on cisplatin-induced mucositis.Male Wistar rats (176-200 g, 6 weeks old) were given cisplatin (5 mg/kg) treated with (300 mg/kg). Histopathological, digestive enzymes activity, oxidative/antioxidant status, proinflammatory/anti-inflammatory cytokines in tissues measured. Next-generation sequencing technologies also performed to investigate gut microbial ecology.D-methionine administration increased villus length crypt depth improved enzyme (leucine aminopeptidase, sucrose alkaline phosphatase) activities brush-border membrane cisplatin-treated (p < 0.05). Furthermore, significantly attenuated oxidative stress inflammatory reaction interleukin-10 levels Cisplatin resulted high relative abundances Deferribacteres Proteobacteria low diversity when compared control groups, only plus D-methionine. markedly comparative Bacteroides caccae, Escherichia coli, Mucispirillum schaedleri, uniformis Desulfovibrio C21-c20, while Lactobacillus almost completely depleted, group. higher Lactobacillus, Lachnospiraceae, Clostridium butyrium than rats. treatment alone number reuteri.D-methionine protects against damage through antioxidative anti-inflammatory effects. By enhancing growth beneficial bacteria (Lachnospiraceae Lactobacillus), attenuates microbiome imbalance caused by maintains homeostasis.

Язык: Английский

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Highly Branched RG-I Domain Enrichment Is Indispensable for Pectin Mitigating against High-Fat Diet-Induced Obesity

Journal of Agricultural and Food Chemistry, Год журнала: 2020, Номер 68(32), С. 8688 - 8701

Опубликована: Июль 7, 2020

Obesity is associated with gut microbiome dysbiosis. Our previous research has shown that highly branched rhamnogalacturonan type I (RG-I)-enriched pectin (WRP, 531.5 kDa, 70.44% RG-I, Rha/(Gal + Ara) = 20) and its oligosaccharide less RG-I [DWRP, 12.1 50.29% 6] are potential prebiotics. The present study conducted to uncover the impact of content, molecular size, branch degrees on inhibiting effect high-fat diet (HFD)-induced obesity. commercial (CP, 496.2 35.77% 6), WRP, DWRP were orally administered HFD-fed C57BL/6J mice (100 mg kg–1 d–1) determine their individual effects WRP significantly prevented bodyweight gain, insulin resistance, inflammatory responses in mice. No obvious anti-obesity was observed either CP or supplementation. A mechanistic revealed could not enhance diversity microbiota, while treatment positively modulated microbiota obese by increasing abundance Butyrivibrio, Roseburia, Barnesiella, Flavonifractor, Acetivibrio, Clostridium cluster IV. Furthermore, promoted browning white adipose tissues mice, did not. can attenuate HFD-induced obesity modulation lipid metabolism. Highly domain enrichment essential for mitigating against

Язык: Английский

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Functions predict horizontal gene transfer and the emergence of antibiotic resistance

Science Advances, Год журнала: 2021, Номер 7(43)

Опубликована: Окт. 22, 2021

Phylogenetic distance, shared ecology, and genomic constraints are often cited as key drivers governing horizontal gene transfer (HGT), although their relative contributions unclear. Here, we apply machine learning algorithms to a curated set of diverse bacterial genomes tease apart the importance specific functional traits on recent HGT events. We find that content accurately predicts network [area under receiver operating characteristic curve (AUROC) = 0.983], performance improves further (AUROC 0.990) for transfers involving antibiotic resistance genes (ARGs), highlighting machinery, niche-specific, metabolic functions. high-probability not-yet detected ARG events almost exclusive human-associated bacteria. Our approach is robust at predicting networks pathogens, including Acinetobacter baumannii Escherichia coli, well within localized environments, such an individual’s gut microbiome.

Язык: Английский

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Enhanced cultured diversity of the mouse gut microbiota enables custom-made synthetic communities

Cell Host & Microbe, Год журнала: 2022, Номер 30(11), С. 1630 - 1645.e25

Опубликована: Окт. 7, 2022

Microbiome research needs comprehensive repositories of cultured bacteria from the intestine mammalian hosts. We expanded mouse intestinal bacterial collection (www.dsmz.de/miBC) to 212 strains, all publicly available and taxonomically described. This includes strain-level diversity, small-sized bacteria, previously undescribed taxa (one family, 10 genera, 39 species). enabled metagenome-educated prediction synthetic communities (SYNs) that capture key functional differences between microbiomes, notably identifying associated with either resistance or susceptibility DSS-induced colitis. Additionally, nine species were used amend Oligo-Mouse Microbiota (OMM)12 model, yielding OMM19.1 model. The added strains compensated for phenotype OMM12 specific pathogen-free mice, including body composition immune cells in lymphoid tissues. Ready-to-use OMM stocks are future studies. In conclusion, this work improves our knowledge gut microbiota diversity mice enables studies via modular use isolates.

Язык: Английский

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