Thinking beyond Vaccination: Promising Add-On Strategies to Active Immunization and Vaccination in Pandemics - a Mini-Review DOI Open Access

Franz Tatzber,

Willibald Wonisch, Ulrike Resch

и другие.

Опубликована: Май 17, 2023

There is little doubt that final victories over pandemics, such as COVID-19, are attributed to herd immunity, either through post-disease convalescence or active immunization of a high percentage the world's population with vaccines, demonstrating protection from infection and transmission, being available in large quantities at reasonable prices. However, it assumable humans immune defects suppression, e.g., consequence allograft transplantation, cannot be immunized actively nor produce sufficient responses prevent SARS-CoV-2 infections. These subjects desperately need other strategies, sophisticated measures immunization. Hypertonic salt solutions attack vulnerable core areas viruses, i.e., denatures surface proteins thus prohibits virus penetration somatic cells. It has ensured not affected by denaturation regarding this unspecific protection. Impregnating filtering facepieces hypertonic straightforward way inactivate viruses potential pathogens. As result contact crystals on facepiece, these pathogens become denatured inactivated almost quantitatively. Such strategy could easily applied fight against COVID-19 pandemic ones may occur future. Another possible tool passive antibodies SARS-CoV-2, preferably human origin. can harvested patients´ sera, which have successfully survived their infection. The disadvantage rapid decrease immunoglobulin titer after ends overcome immortalizing antibody-producing B-cells via fusion with, mouse myeloma resulting monoclonal then origin in, least theoretically, unlimited amounts. Finally, dry blood spots valuable for surveilling population´s immunity.

Язык: Английский

Low pre-infection levels of neutralizing antibody in breakthrough infections after bivalent BA.4-5 vaccine and practical application of dried blood spots DOI Creative Commons
Hitoshi Kawasuji,

Yoshitomo Morinaga,

Hideki Tani

и другие.

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 30, 2024

ABSTRACT The level of neutralizing antibodies required to confer protection against COVID-19 breakthrough infections (BIs) is unclear, and the ability know immune status individuals rapidly changing endemic variants limited. We assessed longitudinal serum anti-RBD antibody levels activities (NTs) Omicron BA.5 XBB.1.5 in healthcare workers following fourth monovalent fifth bivalent BA.4-5 vaccines. occurrence BIs was also followed, pre-infection were compared between patients who developed BI those did not. In addition, we collected whole blood samples on same day as sera stored them filter papers (nos. 545, 590, 424) for up two months, then measured their NTs using dried spots (DBS) eluates, with paired sera. Pre-infection lower than not, but not different them. below 50% 200-fold diluted might be one indicators high risk BI. However, at 6 months after dose vaccine this threshold almost half infection-naïve participants. DBS eluates strongly correlated sera, time temperature stability varied type paper; no. 545 paper found most suitable NT evaluation.

Язык: Английский

Процитировано

0
Unlocking the Potential of Dried Blood Spot Sampling for SARS-CoV-2 Antibody Detection: A Pathway to Efficient Pandemic Surveillance DOI Creative Commons

Ahmad M Alharbi

Diagnostic Microbiology and Infectious Disease, Год журнала: 2024, Номер 111(1), С. 116581 - 116581

Опубликована: Ноя. 4, 2024

The current SARS-CoV-2 pandemic has exerted unprecedented pressure on global healthcare systems, with an urgent demand for widespread testing to monitor virus spread and contribute disease control.

Язык: Английский

Процитировано

0
Elevated binding and functional antibody responses to SARS-CoV-2 in infants versus mothers DOI Creative Commons
Caitlin I. Stoddard, Kevin Sung, Zak A. Yaffe

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Фев. 7, 2023

Infant antibody responses to viral infection can differ from those in adults. However, data on the specificity and function of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies infants, direct comparisons between infants adults are limited. We characterized binding functionality convalescent plasma postpartum women their infected with SARS-CoV-2 a vaccine-naïve prospective cohort Nairobi, Kenya. Antibody titers against Spike, receptor domain N-terminal domain, Spike-expressing cell-surface staining levels were significantly higher than mothers. Plasma mothers bound similar regions Spike S2 subunit, including fusion peptide (FP) stem helix-heptad repeat 2. displayed more consistent escape pathways FP region compared Finally, had antibody-dependent cellular cytotoxicity (ADCC), though, surprisingly, neutralization similar. These results suggest develop distinct functional repertoires reveal age-related differences humoral immunity that could be relevant protection COVID-19 disease outcomes.

Язык: Английский

Процитировано

0
Thinking beyond Vaccination: Promising Add-On Strategies to Active Immunization and Vaccination in Pandemics - a Mini-Review DOI Open Access

Franz Tatzber,

Willibald Wonisch, Ulrike Resch

и другие.

Опубликована: Май 10, 2023

There is little doubt that final victories over pandemics, such as COVID-19, are attributed to herd immunity, either through post-disease convalescence or active immunization of a high percentage the world's population with vaccines, demonstrating protection from infection and transmission, being available in large quantities at reasonable prices. However, it assumable humans immune defects suppression, e.g., consequence allograft transplantation, cannot be immunized actively nor produce sufficient responses prevent SARS-CoV-2 infections. These subjects desperately need other strategies, sophisticated measures immunization. Hypertonic salt solutions attack vulnerable core areas viruses, i.e., denatures surface proteins thus prohibits virus penetration somatic cells. It has ensured not affected by denaturation regarding this unspecific protection. Impregnating filtering facepieces hypertonic straightforward way inactivate viruses potential pathogens. As result contact crystals on facepiece, these pathogens become denatured inactivated almost quantitatively. Such strategy could easily applied fight against COVID-19 pandemic ones may occur future. Another possible tool passive antibodies SARS-CoV-2, preferably human origin. can harvested patients´ sera, which have successfully survived their infection. The disadvantage rapid decrease immunoglobulin titer after ends overcome immortalizing antibody-producing B-cells via fusion with, mouse myeloma resulting monoclonal then origin in, least theoretically, unlimited amounts. Finally, dry blood spots valuable for surveilling population´s immunity.

Язык: Английский

Процитировано

0
Thinking beyond Vaccination: Promising Add-On Strategies to Active Immunization and Vaccination in Pandemics - a Mini-Review DOI Open Access

Franz Tatzber,

Willibald Wonisch, Ulrike Resch

и другие.

Опубликована: Май 17, 2023

There is little doubt that final victories over pandemics, such as COVID-19, are attributed to herd immunity, either through post-disease convalescence or active immunization of a high percentage the world's population with vaccines, demonstrating protection from infection and transmission, being available in large quantities at reasonable prices. However, it assumable humans immune defects suppression, e.g., consequence allograft transplantation, cannot be immunized actively nor produce sufficient responses prevent SARS-CoV-2 infections. These subjects desperately need other strategies, sophisticated measures immunization. Hypertonic salt solutions attack vulnerable core areas viruses, i.e., denatures surface proteins thus prohibits virus penetration somatic cells. It has ensured not affected by denaturation regarding this unspecific protection. Impregnating filtering facepieces hypertonic straightforward way inactivate viruses potential pathogens. As result contact crystals on facepiece, these pathogens become denatured inactivated almost quantitatively. Such strategy could easily applied fight against COVID-19 pandemic ones may occur future. Another possible tool passive antibodies SARS-CoV-2, preferably human origin. can harvested patients´ sera, which have successfully survived their infection. The disadvantage rapid decrease immunoglobulin titer after ends overcome immortalizing antibody-producing B-cells via fusion with, mouse myeloma resulting monoclonal then origin in, least theoretically, unlimited amounts. Finally, dry blood spots valuable for surveilling population´s immunity.

Язык: Английский

Процитировано

0