Revealing the anti-senescence effects and related mechanisms of flavonoid extracts from the buds of Wikstroemia chamaedaphne Meisn on D-galactose-induced PC12 cells based on network pharmacology and transcriptomics
Future Journal of Pharmaceutical Sciences,
Год журнала:
2025,
Номер
11(1)
Опубликована: Март 10, 2025
Abstract
Background
Natural
products
are
an
important
source
of
drugs
or
lead
compounds
for
the
treatment
senescence.
The
buds
Wikstroemia
chamaedaphne
Meisn
a
traditional
Chinese
medicine
to
cure
edema,
schizophrenia
and
epilepsy.
A
flavonoid
extract
W.
(FEW)
was
prepared
from
methanolic
by
our
group
previously,
which
including
eight
flavonoids
with
content
(55.19
±
0.32)
%.
In
this
study,
anti-senescence
effects
related
mechanisms
FEW
on
D-galactose-induced
PC12
cells
were
investigated
first
time.
Results
High
doses
D-galactose
could
induce
cell
senescence,
whereas
delay
senescence
decreasing
SA-β-gal
positivity,
increasing
SOD
activity,
reducing
MDA
levels,
improving
morphology,
inhibiting
cycle
arrest
down-regulating
expression
senescence-related
proteins
P16,
P21
P53.
Subsequently,
potential
underlying
elucidated
through
integration
network
pharmacology
transcriptomics.
main
signaling
pathways
involved
found
be
cancer
pathway,
FOXO
PI3k–Akt
AGE–RAGE
protein
digestion
uptake,
etc.
may
pathway
as
revealed
western
blot
experiments.
Conclusion
Our
study
that
has
effects.
This
suggest
acts
agent
age-related
neurological
diseases.
Язык: Английский
Procyanidins for the treatment of Parkinson's disease and ischemic stroke
Journal of Functional Foods,
Год журнала:
2025,
Номер
127, С. 106717 - 106717
Опубликована: Март 12, 2025
Язык: Английский
Enhanced neuroprotection against Alzheimer’s disease by combining <i>Inula viscosa</i> with curcumin: an <i>in vitro</i> study
Archives of Medical Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 21, 2025
Introduction
Alzheimer’s
disease
(AD),
the
most
prevalent
type
of
dementia
among
older
individuals,
is
characterized
by
severe
neurodegeneration
with
symptoms
progressive
loss
cognitive
capacity.
We
aimed
to
investigate
effects
curcumin
(Curc)
and
Inula
viscosa
(IV)
plant
extracts
on
apoptosis
oxidative
stress
in
an
amyloid-β
(Aβ<i></i>)-induced
vitro
AD
model.
Material
methods
The
human
glioblastoma
cell
line
(U87)
was
treated
Aβ
produce
Treatment
groups
were:
+
Curc,
IV,
IV
Curc.
Cell
viability
assay
performed
analyze
cytotoxicity.
Caspase-3
(CASP3)
levels
were
measured
evaluate
apoptosis.
For
stress,
glutathione
peroxidase
(GPx),
catalase
(CAT),
superoxide
dismutase
(SOD),
total
antioxidant
(TAS),
oxidant
(TOS)
analyzed
spectrophotometrically.
Results
Combinatory
application
Curc
post-Aβ
treatment
increased
U87
cells.
Aβ-upregulated
CASP3
markedly
reduced
upon
co-treatment.
Anti-oxidant
parameters,
i.e.,
SOD,
CAT,
GPx,
TAS
levels,
significantly
elevated
Curc-treated
group
compared
group.
TOS
level
after
exposure
returned
its
basal
Conclusions
co-treatment
apoptosis-related
supported
activity
Aβ-induced
model
can
be
a
promising
therapeutic
strategy
against
AD.
Язык: Английский
Amentoflavone for treating cardiocerebrovascular diseases and neurological disorders
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Ноя. 18, 2024
Amentoflavone
(AME)
is
a
flavonoid
compound
found
in
over
120
plants.
Its
extensive
pharmacological
activity
for
treating
cardiocerebrovascular
diseases
and
neurological
disorders
have
attracted
the
attention
of
researchers
recent
years.
However,
owing
to
poor
solubility
low
bioavailability
AME,
it
has
not
been
developed
as
drug
these
diseases.
This
review
focuses
on
two
aspects
AME:
First,
provides
detailed
summary
introduction
AME
based
its
chemical
structure,
physicochemical
properties,
plant
sources,
extraction
purification
methods,
administration
systems,
pharmacokinetic
properties.
Second,
summarizes
effects
disorders,
specific
mechanisms.
aims
promote
use
disorders.
exhibits
multiple
activities,
indicating
potential
natural
Further
studies
pharmacokinetics
toxicology
are
required
ensure
safety
efficacy.
Язык: Английский