Trends in Genetics, Год журнала: 2023, Номер 39(5), С. 347 - 357
Опубликована: Март 28, 2023
Язык: Английский
BMC Biology, Год журнала: 2020, Номер 18(1)
Опубликована: Авг. 11, 2020
Abstract Background Gene drives based on CRISPR-Cas9 technology are increasingly being considered as tools for reducing the capacity of mosquito populations to transmit malaria, and one most promising options is driving endonuclease genes that reduce fertility female mosquitoes. In particular, there much interest in constructs target conserved doublesex ( dsx ) gene such emergence functional drive-resistant alleles unlikely. Proof principle these can lead substantial population suppression has been obtained cages, they evaluated use sub-Saharan Africa. Here, we simulation modelling understand factors affecting spread this type drive over a million-square kilometre area West Africa containing environmental social heterogeneity. Results We found targeting could reductions malaria vector regional scale. The exact level influenced by additional fitness costs transgene somatic expression Cas9, its deposition sperm or eggs leading damage zygote. absence costs, emergent restore fertility, across study predicted stabilise at ~ 95% 4 years after releases commence. Small do not greatly affect levels suppression, though if females whose offspring construct drops more than 40%, then less efficient. show potential allele with high be enhanced engineering it also express male bias progeny transgenic males. Irrespective strength allele, spatial model predicts somewhat equivalent non-spatial models, particular highly seasonal regions where dry season stochasticity reduces efficiency. explored robustness results uncertainties ecology, their method surviving dispersal rates. Conclusions presented here indicates considerable achieved within few using sterility drive, impact likely heterogeneous space time.
Язык: Английский
Процитировано
93
Annual Review of Entomology, Год журнала: 2020, Номер 66(1), С. 407 - 434
Опубликована: Окт. 9, 2020
Insects play important roles as predators, prey, pollinators, recyclers, hosts, parasitoids, and sources of economically products. They can also destroy crops; wound animals; serve vectors for plant, animal, human diseases. Gene drive-a process by which genes, gene complexes, or chromosomes encoding specific traits are made to spread through wild populations, even if these result in a fitness cost carriers-provides new opportunities altering populations benefit humanity the environment ways that species sustainable. drive be used alter genetic composition an existing population, referred population modification replacement, bring about suppression elimination. We describe technologies under consideration, progress has been made, remaining technological hurdles, particularly with respect evolutionary stability our ability control ultimate fate genes introduced into populations.
Язык: Английский
Процитировано
77
Trends in Genetics, Год журнала: 2022, Номер 38(7), С. 708 - 723
Опубликована: Март 21, 2022
Safe and sustainable approaches for mosquito control are critical due to the global increasing burden of mosquito-transmitted diseases.Novel based on symbionts currently proposed modify or suppress populations Wolbachia-based methods have already achieved some success in field trials.Transgenic mosquitoes carrying gene drives that spread through a promising approach block disease transmission vector species.Transgenic-based potentially offer more power flexibility, but symbiont-based usually socially accepted well-developed. Mosquitoes bring health problems by transmitting parasites viruses such as malaria dengue. Unfortunately, current insecticide-based strategies only moderately effective because high cost resistance. Thus, scalable, sustainable, cost-effective needed mosquito-borne control. Symbiont-based genome engineering-based provide new tools show promise meeting these criteria, enabling modification suppression approaches. Symbiotic bacteria like Wolbachia maternally inherited manipulate host reproduction enhance their vertical transmission. Genome drive methods, which genetically altered alleles throughout wild populations, also proving be powerful laboratory. Here, we review latest developments both drive-based methods. We describe notable similarities, well distinctions obstacles, relating technologies. can found almost anywhere world, tropics subtropics, half world's population is under threat pathogens dengue virus (DENV), Zika (ZIKV), chikungunya (CHIKV), yellow fever, West Nile (WNV), malaria, filarial nematodes [1.World Health Organization Working Overcome Global Impact Neglected Tropical Diseases: First WHO Report Diseases. WHO, 2010Google Scholar,2.Tahir D. et al.Vector-borne nematode diseases pets humans Mediterranean Basin: an update.Vet. World. 2019; 12: 1630-1643Crossref PubMed Scopus (15) Google Scholar]. For example, DENV incidence has grown over 30-fold past 50 years, now reaching about 400 million cases per year [3.Bhatt S. al.The distribution dengue.Nature. 2013; 496: 504-507Crossref (5268) The recent ZIKV outbreak resulted hundreds thousands infections large-scale social economic disruption [4.World Virus Outbreak Response: Interim Report. 2016Google While falling southeast Asia, rising other parts world remain 'unacceptably high' according World [5.Guglielmi G. Malaria worldwide.Nature. (Published online December 4, 2019. https://doi.org/10.1038/d41586-019-03746-3)Crossref Re-emergence expansion many factors, including increased urbanization travel trade, climate change, land use pattern changes, unreliable piped water supply [6.World Vector Control Response 2017-2030. 2017Google Current strategies, long-lasting insecticide-treated bed nets, chemical insecticides, environmental management [7.Schreck C.E. Permethrin dimethyl phthalate tent fabric treatments against Aedes aegypti.J. Am. Mosq. Assoc. 1991; 7: 533-535PubMed Scholar], been unable address genetic behavioral resistance interventions [8.Succo T. al.Autochthonous Nîmes, South France, July September 2015.Euro Surveill. 2016; 21: 21Crossref (0) In addition, unintended effect important nontarget insects, pollinators [9.Ware G.W. Effects pesticides organisms.Residue Rev. 1980; 76: 173-201Crossref (49) new, urgently diseases. response this growing need, number novel technologies expanded years. Many involve release aim achieve (see Glossary) type mosquitoes. Population reduce eliminate populations. Such include sterile insect technique (SIT), incompatible (IIT), transgenic-based technologies, where insects mate with sizes next generation. Gene approaches, increase frequency multiple generations, could after single refractory resistant prevent pathogen symbiont into enable result entire becoming pathogen. review, summarize symbiont-infected transgenic focusing different varieties capabilities. early symbiont-mediated [10.Laven H. Eradication Culex pipiens fatigans cytoplasmic incompatibility.Nature. 1967; 216: 383-384Crossref (275) Scholar] was introduction nonmodified microorganisms competence (Box 1). most extensively studied system natural It may common intracellular endosymbiont arthropods nematodes, 60% all harboring [11.Hilgenboecker K. al.How species infected Wolbachia?--A statistical analysis data.FEMS Microbiol. Lett. 2008; 281: 215-220Crossref transmitted vertically from mother offspring maximize manipulating feminization, parthenogenesis, male killing, and/or incompatibility (CI). CI induced when Wolbachia-infected males uninfected females, results nonviable offspring. inhibit infection DENV, ZIKV, arboviruses, parasite (Figure 1, Key figure ) [12.Bian al.Wolbachia invades Anopheles stephensi induces refractoriness Plasmodium infection.Science. 340: 748-751Crossref (289) Scholar, 13.Dutra H.L. blocks circulating isolates Brazilian aegypti mosquitoes.Cell Host Microbe. 19: 771-774Abstract Full Text PDF 14.Hughes G.L. virulent human falciparum gambiae.PLoS Pathog. 2011; 7e1002043Crossref (214) Transfected native used Scholar,15.Zheng X. al.Incompatible techniques combined mosquitoes.Nature. 572: 56-61Crossref (212) Interestingly, species, aegypti, naturally free [16.Gloria-Soria A. al.Lack evidence (Diptera: Culicidae).J. Med. Entomol. 2018; 55: 1354-1356PubMed Scholar,17.Ross P.A. al.An elusive endosymbiont: does occur aegypti?.Ecol. Evol. 2020; 10: 1581-1591Crossref (27) providing open niche infection. there conjecture if major vectors truly [18.Chrostek E. Gerth M. Is gambiae Wolbachia?.mBio. 10e00784-19Crossref (24) reports indicate Anopheline possess high-density [19.Walker al.Stable moucheti demeilloni mosquitoes.Curr. Biol. 2021; 31: 2310-2320Abstract (12) This offers renewed medically relevant strains adapted environment. Several reviews covered progress exploit bacterium [20.Flores H.A. O'Neill S.L. Controlling vector-borne releasing modified mosquitoes.Nat. 16: 508-518Crossref (145) 21.Wang G.-H. al.Combating using technologies.Nat. Commun. 4388Crossref 22.Caragata E.P. translational science: controlling pathogens.Trends Parasitol. 37: 1050-1067Abstract Scholar].Box 1Using strategiesThe increasingly emerging interactions among hosts, infection, inspiring development [111.Gupta Nair Dynamics insect-microbiome interaction influence microbial symbiont.Front. 11: 1357Crossref (25) Most importantly, shows potential minimize problem cause minimal side effects application includes: (i) delivering directly disrupt physiology display antipathogen effects; (ii) express effector molecules, then engineered so decreased [112.Gao al.Mosquito microbiota implications control.Trends 36: 98-111Abstract (28) vectorial capacity [113.Cansado-Utrilla C. microbiome capacity: rich discovery translation.Microbiome. 9: 111Crossref (9) I).Figure 1Key figure. control.Show full caption(A) Natural bacteria-based (CI) Serratia Y1 Asaia induce immunity slow development. secondary metabolites ureilytica Su_YN1 Chromobacterium responsible antiplasmodial (DENV)-2. (B) fungus-based Wickerhamomyces anomalus secreting toxin protein Beauveria bassiana DENV-2 (ZIKV) activity inducing system. (C) insect-specific (Eilat virus, cell fusing agent Phasi Charoen-like Negevirus) arbovirus development, either alone combination. (D) Engineered AS1 proteins Dirofilaria immitis. (E) Metarhizium pingshaense-infected shorter life spans reproductive output than (F) virus-based Negevirus expressed anti-chikungunya (CHIKV) antibody CHIKV replication.View Large Image Figure ViewerDownload Hi-res image Download (PPT) I). (A) replication. Besides Wolbachia, research made rapid recently 1A,B). field-caught sinensis berghei modulating genes [23.Bai L. al.A gut symbiotic marcescens renders activation immune responses.Front. 1580Crossref interact [24.Cappelli al.Asaia activates eliciting anti-Plasmodium response: control.Front. Genet. 836Crossref (20) Symbionts not interfere pathogens, they directly. secretes antimalarial lipase kills at stages, effectively preventing [25.Gao via secretion lipase.Nat. 6: 806-817Crossref (6) [26.Wu P. commensal promotes permissiveness arboviruses.Cell 25: 101-112Abstract (76) Likewise, inhibits midgut growth displays entomopathogenic larvae adults. Romidepsin might metabolite [27.Saraiva R.G. al.Chromobacterium spp. mediate histone deacetylase inhibitor romidepsin.Sci. Rep. 8: 6176Crossref (23) Scholar]; aminopeptidase interferes attachment degradation Flavivirus E [28.Saraiva al.Aminopeptidase secreted sp. Panama degrading protein.PLoS Negl. Trop. Dis. 12e0006443Crossref fungi yeast killer strong developmental stages [29.Cappelli al.Killer yeasts exert anti-plasmodial activities mice.Parasite Vector. 329Crossref Toll/Jak-Stat pathways reduces [30.Dong Y. fungus activate toll JAK-STAT pathway-controlled anti-dengue aegypti.Insect Biochem. Mol. 2012; 42: 126-132Crossref (80) albopictus [31.Deng S.Q. al.Beauveria virus.J. Pest. Sci. 92: 781-789Crossref (8) Beyond fungi, (ISVs) arboviruses. Cell (CFAV) recognized ISV cells phenotype [32.Stollar V. Thomas V.L. An line (Peleg) causes fusion cells.Virology. 1975; 64: 367-377Crossref (167) Another ISV, Eilat (EILV), titers delay replication vitro. When were EILV, dissemination delayed heterologous interference mechanism [33.Nasar F. al.Eilat homologous interference.Am. J. Hyg. 2015; 93: 226Google Co-infection ISVs CFAV co-infection [34.Schultz M.J. al.Dual specific limits 518: 406-413Crossref (46) another discovered [35.Vasilakis N. al.Negevirus: taxon wide geographic distribution.J. Virol. 87: 2475-2488Crossref (121) cells, Mayaro [36.Patterson E.I. al.Negeviruses alphaviruses during coinfection.J. 95e0043321Crossref (2) All suggest arboviruses superinfection exclusion, needs further testing population. producing immunomodulatory molecules (termed paratransgenesis) 1C,D). After engineered, it reintroduced arthropod its There requirements candidate symbiont. First, should able stably horizontally maintain long enough [37.Wilke A.B. Marrelli M.T. Paratransgenesis: strategy control.Parasit. Vectors. 342Crossref (97) Second, easily culturable manipulatable, while reducing fitness [38.Lovett B. al.Transgenic rapidly malaria-endemic region Burkina Faso.Science. 364: 894-897Crossref (40) Third, target [39.Wang al.Driving bacteria.Science. 2017; 357: 1399-1402Crossref (116) several candidates shown attributes paratransgenesis (AS1), isolated ovaries, horizontally, facilitating Furthermore, Together, suggests bogorensis populate larval adult organs [40.Favia al.Bacteria genus associate stephensi, Asian malarial vector.Proc. Natl. Acad. U. 2007; 104: 9047-9051Crossref (296) Recently, successfully conditionally scorpine, significantly parasites, displaying reduced compared stain constitutively expressing [41.Shane J.L. al.Blood meal-induced inhibition microbiota.Nat. 4127Crossref (31) More recently, within heartworm immitis [42.Epis al.Chimeric stimulate development.Commun. 3: 105Crossref Notably, keep capability laboratory semi-field conditions Intriguingly, appear antagonistic one another. showed lower densities counterparts, removing enabled [43.Rosso al.Reduced diversity two areas invasion.Sci. 16091Crossref Scholar,44.Hughes al.Native impedes mosquitoes.Proc. 2014; 111: 12498-12503Crossref (137) Genetically pingshaense (Mp-hybrid) coluzzii had lifespans Mp-hybrid higher virulence inoculum load trial Faso Finally, modifying Negevirus, anti-CHIKV antibodies inhibited bacteria, fungus, even existing basis identified compelling genes, cifA cifB, involved induction rescue. models mechanism(s) still elucidated, expression bacterial germline recapitulate phenotype. Expression females rescues CI, intriguingly, appears coexpression cifB required [45.Beckmann J.F. deubiquitylating enzyme incompatibility.Nat. 2: 17007Crossref (181) Scholar,46.LePage D.P. al.Prophage WO Wolbachia-induced 543: 243-247Crossref (218) phenotypes replicated crosses between cif studies examining molecular accomplished flies 46.LePage 47.Shropshire J.D. al.One prophage Drosophila melanogaster.Proc. 115: 4987-4991Crossref (82) 48.Shropshire al.Evolution-guided mutagenesis proteins: identifying CifA's complex functional repertoire essential regions CifB.PLoS 16e1008794Crossref (11) recapitulated wPip (though here, CI) [49.Adams K.L. vector.Nat. M
Язык: Английский
Процитировано
54
G3 Genes Genomes Genetics, Год журнала: 2022, Номер 12(6)
Опубликована: Апрель 8, 2022
Abstract Gene drives are engineered alleles that can bias inheritance in their favor, allowing them to spread throughout a population. They could potentially be used modify or suppress pest populations, such as mosquitoes diseases. CRISPR/Cas9 homing drives, which copy themselves by homology-directed repair drive/wild-type heterozygotes, powerful form of gene drive, but they vulnerable resistance preserve the function target gene. Such prevent successful population suppression. Here, we constructed suppression drive Drosophila melanogaster utilized multiplexed gRNAs inhibit formation functional its female fertility The selected gRNA sites were close together, preventing reduction conversion efficiency. construct reached moderate equilibrium frequency cage populations without apparent alleles. However, fitness cost prevented elimination population, showing importance using highly efficient strategy, even if addressed. Nevertheless, our results experimentally demonstrate viability strategy drives.
Язык: Английский
Процитировано
52
Evolutionary Applications, Год журнала: 2022, Номер 16(1), С. 3 - 21
Опубликована: Дек. 9, 2022
Abstract Evolution has traditionally been a historical and descriptive science, predicting future evolutionary processes long considered impossible. However, predictions are increasingly being developed used in medicine, agriculture, biotechnology conservation biology. Evolutionary may be for different purposes, such as to prepare the future, try change course of evolution or determine how well we understand processes. Similarly, exact aspect evolved population that want predict also differ. For example, could which genotype will dominate, fitness extinction probability population. In addition, there many uses not always recognized such. The main goal this review is increase awareness methods data research fields by showing breadth situations made. We describe diverse share common structure described predictive scope, time scale precision. Then, using examples ranging from SARS‐CoV2 influenza CRISPR‐based gene drives sustainable product formation biotechnology, discuss evolution, factors affect predictability can prevent undesirable directions promote beneficial (i.e. control). hope stimulate collaboration between establishing language predictions.
Язык: Английский
Процитировано
47
Evolutionary Applications, Год журнала: 2020, Номер 14(4), С. 1052 - 1069
Опубликована: Дек. 1, 2020
Abstract CRISPR gene drive systems offer a mechanism for transmitting desirable transgene throughout population purposes ranging from vector‐borne disease control to invasive species suppression. In this simulation study, we assess the performance of several CRISPR‐based underdominance constructs employing toxin‐antidote (TA) principles. These drives disrupt wild‐type version an essential using nuclease (the toxin) while simultaneously carrying recoded antidote). Drives nature allow releases that could be potentially confined desired geographic location. This is because such have nonzero‐invasion threshold frequency required spread through population. We model which target genes are either haplosufficient or haplolethal, promoters with expression restricted germline, additionally result in cleavage activity early embryo maternal deposition, and ubiquitous somatic expression. also study possible architectures, considering both “same‐site” “distant‐site” systems, as well reciprocally targeting drives. Together, these variants provide wide range invasion frequencies options modification Our results suggest TA design flexible confinable strategies.
Язык: Английский
Процитировано
50
Ecology Letters, Год журнала: 2023, Номер 26(7), С. 1174 - 1185
Опубликована: Май 10, 2023
Suppression gene drives bias their inheritance to spread through a population, potentially eliminating it when they reach high frequency. CRISPR homing suppression have already seen success in the laboratory, but several models predict that may be elusive population with realistic spatial structure due extinction-recolonization cycles. Here, we extend our continuous space framework include two competing species or predator-prey pairs. We find both general and mosquito-specific models, predators can facilitate drive-based suppression, albeit at cost of an increased rate drive loss outcomes. These results are robust mosquito seasonal fluctuations. Our study illustrates difficulty predicting outcomes complex ecosystems. However, promising for prospects less powerful successfully eliminate target other pest populations.
Язык: Английский
Процитировано
16
Nature Communications, Год журнала: 2024, Номер 15(1)
Опубликована: Окт. 17, 2024
Gene drives are a promising means of malaria control with the potential to cause sustained reductions in transmission. In real environments, however, their impacts will depend on local ecological and epidemiological factors. We develop data-driven model investigate gene that causes vector population suppression. simulate drive releases sixteen ~ 12,000 km
Язык: Английский
Процитировано
6
EFSA Journal, Год журнала: 2020, Номер 18(11)
Опубликована: Ноя. 1, 2020
Advances in molecular and synthetic biology are enabling the engineering of gene drives insects for disease vector/pest control.Engineered (that bias their own inheritance) can be designed either to suppress interbreeding target populations or modify them with a new genotype.Depending on engineered drive system, theoretically, genetic modification interest could spread through persist indefinitely, restricted its persistence.While research applications is advancing at fast pace, it will take several years technological developments move practical deliberate release into environment.Some modified (GDMIs) have been tested experimentally laboratory, but none has assessed small-scale confined field trials open as yet.There concern that GDMIs environment may possible irreversible unintended consequences.As proactive measure, European Food Safety Authority (EFSA) requested by Commission review whether previously published guidelines risk assessment genetically animals (EFSA, 2012 2013), including (GMIs), adequate sufficient GDMIs, primarily vectors, agricultural pests invasive species, environment.Under this mandate, EFSA was not develop GDMIs.In Scientific Opinion, Panel Genetically Modified Organisms (GMO) concludes EFSA's adequate, insufficient characterisation (MC), environmental (ERA) post-market monitoring (PMEM) GDMIs.While MC, ERA PMEM build existing framework GMIs do contain drives, there specific areas where further guidance needed GDMIs.
Язык: Английский
Процитировано
42
PLoS Genetics, Год журнала: 2021, Номер 17(2), С. e1009385 - e1009385
Опубликована: Фев. 18, 2021
Gene drive elements promote the spread of linked traits, providing methods for changing composition or fate wild populations. Drive mechanisms that are self-limiting attractive because they allow control over duration and extent trait in time space, reversible through natural selection as wanes. Self-sustaining Cleave Rescue ( ClvR ) include a DNA sequence-modifying enzyme such Cas9/gRNAs disrupts endogenous versions an essential gene, tightly recoded version gene resistant to cleavage (the ), Cargo. spreads by creating loss-of-function (LOF) conditions which those without die lack functional copies gene. We use modeling show when -Cargo one both components required LOF allele creation (Cas9 gRNA) reside at different locations (split is due progressive decrease Cas9 frequency, thus opportunities alleles, occurs. Importantly, strength can be extended measured manner—which still self-limiting—by moving two close enough each other experience some degree linkage. With linkage, transiently experiences hitchhiking with until linkage disequilibrium between disappears, function recombination frequency number generations, novel point control. implement split Drosophila , key on chromosomes. Cargo/ /gRNAs high Cas9-dependent manner, while decreases. These observations measured, transient drive, coupled loss future potential, achieved using simple toolkit make up elements—Cas9 gRNAs /Cargo.
Язык: Английский
Процитировано
33