The structural basis of odorant recognition in insect olfactory receptors

Nature, Год журнала: 2021, Номер 597(7874), С. 126 - 131

Опубликована: Авг. 4, 2021

Abstract Olfactory systems must detect and discriminate amongst an enormous variety of odorants 1 . To contend with this challenge, diverse species have converged on a common strategy in which odorant identity is encoded through the combinatorial activation large families olfactory receptors 1–3 , thus allowing finite number to vast chemical world. Here we offer structural mechanistic insight into how individual receptor can flexibly recognize odorants. We show that Mh OR5 from jumping bristletail 4 Machilis hrabei assembles as homotetrameric odorant-gated ion channel broad tuning. Using cryo-electron microscopy, elucidated structure multiple gating states, alone complex two its agonists—the eugenol insect repellent DEET. Both ligands are recognized distributed hydrophobic interactions within same geometrically simple binding pocket located transmembrane region each subunit, suggesting logic for promiscuous sensitivity receptor. Mutation residues lining predictably altered DEET broadly reconfigured receptor’s Together, our data support model share determinants binding, shedding light molecular recognition mechanisms ultimately endow system immense discriminatory capacity.

Язык: Английский

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Structural basis for odorant recognition of the insect odorant receptor OR-Orco heterocomplex

Science, Год журнала: 2024, Номер 384(6703), С. 1453 - 1460

Опубликована: Июнь 13, 2024

Insects detect and discriminate a diverse array of chemicals using odorant receptors (ORs), which are ligand-gated ion channels comprising divergent odorant-sensing OR conserved receptor co-receptor (Orco). In this work, we report structures the

Язык: Английский

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Structural basis of odor sensing by insect heteromeric odorant receptors

Science, Год журнала: 2024, Номер 384(6703), С. 1460 - 1467

Опубликована: Июнь 13, 2024

Most insects, including human-targeting mosquitoes, detect odors through odorant-activated ion channel complexes consisting of a divergent odorant-binding subunit (OR) and conserved co-receptor (Orco). As basis for understanding how odorants activate these heteromeric receptors, we report here cryo–electron microscopy structures two different odorant receptor containing ORs from disease-vector mosquitos Aedes aegypti or Anopheles gambiae . These reveal an unexpected stoichiometry one OR to three Orco subunits. Comparison in odorant-bound unbound states indicates that binding the sole is sufficient open pore, suggesting mechanism activation conceptual framework evolution insect sensitivity.

Язык: Английский

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Evolution, developmental expression and function of odorant receptors in insects

Journal of Experimental Biology, Год журнала: 2020, Номер 223(Suppl_1)

Опубликована: Фев. 1, 2020

ABSTRACT Animals rely on their chemosensory system to discriminate among a very large number of attractive or repulsive chemical cues in the environment, which is essential respond with proper action. The olfactory sensory systems insects share significant similarities those vertebrates, although they also exhibit dramatic differences, such as molecular nature odorant receptors (ORs): insect ORs function heteromeric ion channels common Orco subunit, unlike G-protein-coupled found vertebrates. Remarkable progress has recently been made understanding evolution, development and receptor neurons (ORNs). These studies have uncovered diversity species, including eusocial that extensively sensing pheromones for social communication. However, further studies, notably functional analyses, are needed improve our origins Orco–OR system, mechanisms ORN fate determination, extraordinary behavioral responses cues.

Язык: Английский

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Odorant Receptors for Detecting Flowering Plant Cues Are Functionally Conserved across Moths and Butterflies

Molecular Biology and Evolution, Год журнала: 2020, Номер 38(4), С. 1413 - 1427

Опубликована: Ноя. 23, 2020

Odorant receptors (ORs) are essential for plant-insect interactions. However, despite the global impacts of Lepidoptera (moths and butterflies) as major herbivores pollinators, little functional data available about ORs involved in plant-volatile detection. Here, we initially characterized plant-volatile-sensing function(s) 44 from cotton bollworm Helicoverpa armigera, subsequently conducted a large-scale comparative analysis that establishes how most orthologous have functionally diverged among closely related species whereas some rare conserved. Specifically, our systematic H. armigera cataloged wide scope OR repertoire, also showed HarmOR42 its Spodoptera littoralis ortholog Pursuing this, HarmOR42-orthologous 11 across Glossata suborder confirmed orthologs form unique lineage has undergone strong purifying selection whose members tuned with specificity to phenylacetaldehyde, floral scent component common angiosperms. In vivo studies via knockout support HarmOR42-related host-detection by sensing phenylacetaldehyde. Our work supports these coevolved tube-like proboscis, maintained stability throughout long-term coexistence Thus, beyond providing rich empirical resource delineating precise functions ORs, results enable insect apparently facilitated currently sustain intimate adaptations ecological interactions nectar feeding insects flowering plants.

Язык: Английский

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The diversity and evolution of odorant receptors in beetles (Coleoptera)

Insect Molecular Biology, Год журнала: 2019, Номер 29(1), С. 77 - 91

Опубликована: Авг. 5, 2019

The insect odorant receptors (ORs) are amongst the largest gene families in genomes and primary means by which insects recognize volatile compounds. evolution of ORs is thus instrumental explaining chemical ecology as a model evolutionary biology. However, although have been described from numerous species, their analysis within orders has hindered combination limited genomic information tendency OR family toward rapid divergence, gain, loss. We addressed these issues order Coleoptera through targeted annotation effort that included 1181 one species sister Strepsiptera 10 representing four coleopteran suborders. numbers each varied hundreds to fewer than 10, but could nevertheless be represented scheme nine monophyletic subfamilies. observed many radiations losses genes subfamilies, diversity appeared parallel host breadth study species. some small lineages persisted families, suggesting key function underlie olfactory beetles.

Язык: Английский

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Molecular mechanisms of olfactory detection in insects: beyond receptors

Open Biology, Год журнала: 2020, Номер 10(10)

Опубликована: Окт. 1, 2020

Insects thrive in diverse ecological niches large part because of their highly sophisticated olfactory systems. Over the last two decades, a major focus study insect olfaction has been on role receptors mediating neuronal responses to environmental chemicals. In vivo , these operate specialized structures, called sensilla, which comprise neurons and non-neuronal support cells, extracellular lymph fluid precisely shaped cuticle. While sensilla are inherent odour sensing insects, we only just beginning understand construction function. Here, review recent work that illuminates how odour-evoked activity is impacted by sensillar morphology, biochemistry, accessory signalling molecules physiological crosstalk between cells. These advances reveal multi-layered molecular cellular mechanisms determine selectivity, sensitivity dynamic modulation insects.

Язык: Английский

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Olfactory coding in honeybees

Cell and Tissue Research, Год журнала: 2021, Номер 383(1), С. 35 - 58

Опубликована: Янв. 1, 2021

Abstract With less than a million neurons, the western honeybee Apis mellifera is capable of complex olfactory behaviors and provides an ideal model for investigating neurophysiology circuit basis perception learning. Here, we review most fundamental aspects honeybee’s olfaction: first, discuss which odorants dominate its environment, how bees use them to communicate regulate colony homeostasis; then, describe neuroanatomy circuit; finally, explore cellular molecular mechanisms leading memory formation. The vastity histological, neurophysiological, behavioral data collected during last century, together with new technological advancements, including genetic tools, confirm as attractive research understanding coding

Язык: Английский

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Robust olfactory responses in the absence of odorant binding proteins

eLife, Год журнала: 2019, Номер 8

Опубликована: Окт. 25, 2019

Odorant binding proteins (Obps) are expressed at extremely high levels in the antennae of insects, and have long been believed essential for carrying hydrophobic odorants to odor receptors. Previously we found that when one functional type olfactory sensillum Drosophila was depleted its sole abundant Obp, it retained a robust response (Larter et al., 2016). Here deleted all Obp genes abundantly antennal basiconic sensilla. All six tested types responded robustly odors widely diverse chemical or temporal structure. One mutant gave greater physiological behavioral an odorant affects oviposition. Our results support model which many sensilla can respond absence Obps, Obps not response, but some modulate physiology behavior drives.

Язык: Английский

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Brown marmorated stink bug, Halyomorpha halys (Stål), genome: putative underpinnings of polyphagy, insecticide resistance potential and biology of a top worldwide pest

BMC Genomics, Год журнала: 2020, Номер 21(1)

Опубликована: Март 14, 2020

Abstract Background Halyomorpha halys (Stål), the brown marmorated stink bug, is a highly invasive insect species due in part to its exceptionally high levels of polyphagy. This also nuisance overwintering human-made structures. It has caused significant agricultural losses recent years along Atlantic seaboard North America and continental Europe. Genomic resources will assist with determining molecular basis for this species’ feeding habitat traits, defining potential targets pest management strategies. Results Analysis 1.15-Gb draft genome assembly identified wide variety genetic elements underpinning biological characteristics formidable species, encompassing roles sensory functions, digestion, immunity, detoxification development, all which likely support H. ’ capacity invasiveness. Many genes herein have biomolecular pesticide applications. Conclusions Availability sequence be useful development environmentally friendly pesticides applied concert more traditional, synthetic chemical-based controls.

Язык: Английский

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