Metals in Motion: Understanding Labile Metal Pools in Bacteria
Biochemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 5, 2025
Metal
ions
are
essential
for
all
life.
In
microbial
cells,
potassium
(K+)
is
the
most
abundant
cation
and
plays
a
key
role
in
maintaining
osmotic
balance.
Magnesium
(Mg2+)
dominant
divalent
required
nucleic
acid
structure
as
an
enzyme
cofactor.
Microbes
typically
require
transition
metals
manganese
(Mn),
iron
(Fe),
copper
(Cu),
zinc
(Zn),
although
precise
set
of
metal
needed
to
sustain
life
variable.
Intracellular
pools
can
be
conceptualized
chemically
complex
mixture
rapidly
exchanging
(labile)
ions,
complemented
by
those
reservoirs
that
exchange
slowly
relative
cell
metabolism
(sequestered).
Labile
buffered
transient
interactions
with
anionic
metabolites
macromolecules,
ribosome
playing
major
role.
Sequestered
include
many
metalloproteins,
cofactors,
storage
depots,
some
redeployed
upon
depletion.
Here,
I
review
size,
composition,
dynamics
intracellular
highlight
gaps
understanding.
Язык: Английский
Synthesis and Characterization of a Fluorinated Schiff Base from Benzimidazole and Its Metal Complexes for Antimicrobial and UV-Protective Cotton Fabrics
Coatings,
Год журнала:
2025,
Номер
15(4), С. 380 - 380
Опубликована: Март 24, 2025
Due
to
the
lack
of
UV-protective
properties
for
cotton
textiles
and
potential
cause
microbes
their
users,
we
synthesized
benzimidazole
Schiff
base
derivative
(BZI)
namely
N-((1H-benzo[d]imidazol-2-yl)methyl)-1-(4-fluorophenyl)methanimine
V(III),
Fe(III),
Co(II),
Ni(II),
Cu(II)
complexes
as
UV
protection
antimicrobial
agents
textile.
Several
techniques
investigated
these
compounds:
1H,
13C
NMR,
IR,
UV–Vis,
elemental
analysis,
DTA,
TGA.
The
ligand
behaved
a
bidentate
ligand.
prepared
its
are
used
treat
fabrics
(CFs)
by
immersing
fabric
in
solution
samples
under
ultrasonic.
treated
were
using
IR
SEM-EDX
analysis.
UPF
values
obtained.
results
showed
that
with
Fe(III)
had
excellent
50+.
disc
diffusion
method
evaluated
fabric’s
activity.
antifungal
activities
CFs
demonstrated
Co(II)-BZI-CF
was
active
on
C.
albicans
an
inhibition
zone
12
mm,
while
other
inactive
A.
flavus.
V(III)-BZI-CF
Fe(III)-BZI-CF
no
activity
against
S.
aureus
E.
coli
bacteria
gave
between
10
17
mm.
Unlike
previous
studies
primarily
focused
either
or
metal
separately,
this
research
integrates
both
functionalities
synthesizing
applying
them
textiles,
demonstrating
enhanced
selective
Язык: Английский
The TerC family metal chaperone MeeY enables surfactin export in Bacillus subtilis
Journal of Bacteriology,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 16, 2025
ABSTRACT
TerC
family
proteins
are
widely
conserved
integral
membrane
with
functions
related
to
metal
transport.
In
Bacillus
subtilis
,
the
MeeF
and
MeeY
play
overlapping
roles
in
metalation
of
manganese-requiring
extracellular
enzymes.
interact
secretion
translocon
SecYEG
metalate
either
during
or
after
protein
translocation.
Here,
we
demonstrate
that
swarming
motility
is
dependent
on
MeeY.
This
defect
can
be
complemented
extracellularly
correlated
a
loss
surfactin.
Surfactin
export
mediated
by
SwrC,
an
RND
efflux
pump
previously
shown
co-immunoprecipitation
studies.
The
amendment
growth
medium
manganese
has
long
been
known
enhance
surfactin
production.
We
suggest
model
which
enhanced
MeeY-dependent
lipopeptide
export.
IMPORTANCE
produces
surfactin,
powerful
detergent-like
compound
intercellular
communication,
surface
motility,
as
broad-spectrum
antimicrobial
agent.
Production
cyclic
lipopeptide,
depends
non-ribosomal
peptide
synthase
followed
member
resistance-nodulation-cell
division
(RND)
proteins.
production
additionally
requires
MeeY,
exports
ions
support
function
secreted
metalloenzymes.
propose
interacts
SwrC
facilitate
binding
from
cell.
These
results
may
explain
long-appreciated
role
divalent
industrial
fermentation.
Язык: Английский
Physiological cost of antibiotic resistance: Insights from a ribosome variant in bacteria
Science Advances,
Год журнала:
2024,
Номер
10(46)
Опубликована: Ноя. 15, 2024
Antibiotic-resistant
ribosome
variants
arise
spontaneously
in
bacterial
populations;
however,
their
impact
on
the
overall
physiology
remains
unclear.
We
studied
naturally
arising
antibiotic-resistant
L22*
variant
of
Bacillus
subtilis
and
identified
a
Mg
2+
-dependent
physiological
cost.
Coculture
competition
experiments
show
that
limitation
hinders
growth
more
than
wild
type
(WT),
even
under
antibiotic
pressure.
This
disadvantage
cells
is
not
due
to
lower
abundance
but
rather
reduced
intracellular
levels.
Coarse-grained
elastic-network
modeling
conformational
dynamics
suggests
ribosomes
associate
tightly
with
when
compared
WT.
combined
structural
experimental
measurements
steady-state
model
predict
cellular
adenosine
5′-triphosphate
(ATP)
levels,
which
also
depend
.
Experiments
confirmed
predicted
ATP
drop
limitation,
while
WT
were
less
affected.
Intracellular
for
finite
pool
can
thus
suppress
establishment
an
variant.
Язык: Английский