PLoS Computational Biology,
Год журнала:
2017,
Номер
13(10), С. e1005814 - e1005814
Опубликована: Окт. 25, 2017
How
neuronal
diversity
emerges
from
complex
patterns
of
gene
expression
remains
poorly
understood.
Here
we
present
an
approach
to
understand
electrophysiological
through
by
integrating
pooled-
and
single-cell
transcriptomics
with
intracellular
electrophysiology.
Using
neuroinformatics
methods,
compiled
a
brain-wide
dataset
34
neuron
types
paired
intrinsic
features
publically
accessible
sources,
the
largest
such
collection
date.
We
identified
420
genes
whose
levels
significantly
correlated
variability
in
one
or
more
11
physiological
parameters.
next
trained
statistical
models
infer
cellular
multivariate
patterns.
Such
were
predictive
gene-electrophysiological
relationships
independent
12
visual
cortex
cell
Allen
Institute,
suggesting
that
these
correlations
might
reflect
general
principles
relating
phenotypic
across
very
different
types.
Many
associations
reported
here
have
potential
provide
new
insights
into
how
neurons
generate
functional
diversity,
ion
channel
like
Gabrd
Scn1a
(Nav1.1)
resting
spiking
frequency
are
consistent
known
causal
mechanisms.
Our
work
highlights
promise
inherent
challenges
using
type-specific
mechanistic
origins
diversity.
Frontiers in Neural Circuits,
Год журнала:
2019,
Номер
12
Опубликована: Янв. 21, 2019
It
is
now
accepted
that
neurons
contain
and
release
multiple
transmitter
substances.
However,
we
still
have
only
limited
insight
into
the
regulation
functional
effects
of
this
co-transmission.
Given
there
are
200
or
more
neurotransmitters,
chemical
complexity
nervous
system
daunting.
This
made
more-so
by
fact
their
interacting
can
generate
diverse
non-linear
novel
consequences.
The
relatively
poor
history
pharmacological
approaches
likely
reflects
manipulating
a
will
not
necessarily
mimic
its
roles
within
normal
environment
(e.g.
when
it
acts
in
parallel
with
co-transmitters).
In
article,
co-transmission
discussed
range
systems
(from
invertebrate
lower
vertebrate
models,
up
to
mammalian
peripheral
central
system)
highlight
used,
degree
understanding,
open
questions
future
directions.
Finally,
offer
some
outlines
what
consider
be
general
principles
co-transmission,
as
well
think
most
pressing
aspects
need
addressed
move
forward
our
understanding
Translational Psychiatry,
Год журнала:
2018,
Номер
8(1)
Опубликована: Фев. 23, 2018
The
lateral
habenula
(LHb)
has
a
key
role
in
integrating
variety
of
neural
circuits
associated
with
reward
and
aversive
behaviors.
There
is
limited
information
about
how
the
different
cell
types
neuronal
within
LHb
coordinate
physiological
motivational
states.
Here,
we
report
type
medial
division
(LHbM)
male
rats
that
distinguished
by:
(1)
molecular
signature
for
GABAergic
neurotransmission
(Slc32a1/VGAT)
estrogen
receptor
(Esr1/ERα)
expression,
at
both
mRNA
protein
levels,
as
well
vesicular
glutamate
transporter
Slc17a6/VGLUT2,
which
term
estrogen-receptive
neuron
(GERN);
(2)
its
axonal
projection
patterns,
identified
by
vivo
juxtacellular
labeling,
to
local
midbrain
modulatory
systems;
(3)
somatic
expression
receptors
vasopressin,
serotonin
dopamine,
orexin
2.
This
anatomically
located
receive
afferents
from
(dopamine
serotonin)
hypothalamic
water
energy
homeostasis
(vasopressin
orexin)
circuits.
These
shared
synthase
(aromatase)
VGLUT2,
their
somata
axon
terminals.
We
demonstrate
dynamic
changes
LHbM
VGAT+
density,
dependent
upon
gonadal
functional
status,
closely
correlate
behavior
response
predator
forced
swim
stressors.
findings
suggest
reward-related
glutamatergic
convergent
projecting
pathways
LHbMC
employ
localized
neurosteroid
signaling
mechanism
via
aromatase,
act
switch
GERN
excitation/inhibition
output
prevalence,
influencing
depressive
or
motivated
behavior.
The Journal of Physiology,
Год журнала:
2014,
Номер
593(1), С. 3 - 23
Опубликована: Ноя. 11, 2014
Breathing
in
mammals
is
a
seemingly
straightforward
behaviour
controlled
by
the
brain.
A
brainstem
nucleus
called
preBötzinger
Complex
sits
at
core
of
neural
circuit
generating
respiratory
rhythm.
Despite
discovery
this
microcircuit
almost
25
years
ago,
mechanisms
controlling
breathing
remain
elusive.
Given
apparent
simplicity
and
well-defined
nature
regulatory
behaviour,
identification
much
circuitry,
ability
to
study
vitro
as
well
vivo,
many
neuroscientists
physiologists
are
surprised
that
rhythm
generation
still
not
understood.
Our
view
conventional
rhythmogenic
involving
pacemakers,
inhibition
or
bursting
problematic
simplifying
assumptions
commonly
made
for
vertebrate
circuits
ignore
consequential
detail.
We
propose
novel
emergent
govern
That
mammalian
function
basic
arises
from
complex
dynamic
molecular,
synaptic
neuronal
interactions
within
diverse
highlights
challenges
understanding
control
behaviours,
(considerably)
more
elaborate
than
breathing.
suggest
inimitably
tractable
may
inspire
general
strategies
elucidating
other
microcircuits.
