OncoImmunology, Год журнала: 2024, Номер 13(1)
Опубликована: Авг. 26, 2024
Disrupting mitochondrial function in malignant cells is a promising strategy to enhance anticancer immunity. We have recently demonstrated that depriving colorectal cancer of serine results dysfunction coupled with the cytosolic accumulation DNA and consequent activation CGAS- STING-dependent tumor-targeting immune responses.
Язык: Английский
Hypertension Research, Год журнала: 2024, Номер 47(9), С. 2456 - 2470
Опубликована: Июль 4, 2024
Abstract The contrasting relationships of plant and animal protein intake with blood pressure (BP) may be partially attributed to the differential non-protein (e.g., saturated fat fibre) amino acid (AA) compositions. This study determined whether were related metabolomic profiles associated BP. included 1008 adults from African-PREDICT (aged 20–30 years). Protein was using 24-h dietary recalls. Twenty-four-hour ambulatory BP measured. Amino acids acylcarnitines analysed in spot urine samples liquid chromatography-tandem mass spectrometry-based metabolomics. Participants a low plant, high had higher SBP (by 3 mmHg, p = 0.011) than those (low-risk group). We found that mediated by BMI intake, which independently SBP. therefore not multiple regression analysis after adjusting for confounders. In low-risk group, methionine (Std. β −0.217; 0.034), glutamic −0.220; 0.031), glycine −0.234; 0.025), proline −0.266; 0.010) inversely SBP, beta-alanine −0.277; 0.020) DBP. Ultimately diet contribute means increased intake. Conversely, levels urinary AAs observed consuming rich lower
Язык: Английский
Процитировано
4
Journal of Hazardous Materials, Год журнала: 2024, Номер unknown, С. 135789 - 135789
Опубликована: Сен. 1, 2024
Язык: Английский
Процитировано
3
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(2), С. 684 - 684
Опубликована: Янв. 15, 2025
Macular telangiectasia type 2 (MacTel) is a slowly progressive macular disorder that often diagnosed late due to the gradual onset of vision loss. Recent advances in diagnostic techniques have facilitated earlier detection and shown MacTel more common than initially thought. The disease genetically complex, multiple variants contribute incrementally overall risk. familial occurrence prompted investigation genetic background MacTel. To better understand molecular milieu disease, literature review clinical reports publications investigating factors was performed. date, disease-associated been found genes involved amino acid (glycine/serine) metabolism transport, urea cycle, lipid metabolism, retinal vasculature thickness. Variants implicated sphingolipid fatty acid/steroid/retinol patients with neurological disorders who also Retinal involves complex biochemical processes are essential for maintaining high energy requirements retina. Genetic alterations can disrupt key metabolic pathways, leading cell degradation subsequent loss characterizes several disorders, including This article summarizes findings may allow be further investigated as an inherited disorder.
Язык: Английский
Процитировано
0
Metabolites, Год журнала: 2025, Номер 15(2), С. 86 - 86
Опубликована: Фев. 1, 2025
Introduction and objective: This literature review aims to provide an overview of the progress in metabolomic assessment animal cell models humans with diabetic neuropathy (DN). Methods: Metabolomics has emerged as important approach for investigating, identifying, describing biomarkers related DN. None yet been validated use clinical practice. Results: DN induced significant alterations energy metabolism carbohydrates, lipids, amino acids, peptides, proteins. Several treatments DN, evaluated using metabolomics, were proved have promising results. Conclusions: The ideal metabolite or set metabolites that could be used should identify patients diabetes prone develop those severe forms sensory loss, associated risk ulcerations amputation. Another potential a might indicator treatment response trials agents disease-modifying properties.
Язык: Английский
Процитировано
0
Cellular & Molecular Biology Letters, Год журнала: 2025, Номер 30(1)
Опубликована: Фев. 19, 2025
Abstract Background Cyclophosphamide (CTX) is the first-line medication for treatment of breast cancer, although it potentially leads to severe ovarian dysfunction and even premature failure (POF). However, mechanism CTX-induced POF remains unclear. Mesenchymal stem cell-based therapy has been wildly used treating numerous diseases. Therefore, our study aims elucidate underlying explore therapeutic effect human urine cells (hUSCs) in POF. Methods or granulosa cell (GCs) apoptosis were treated with hUSCs their exosomes vitro vivo. Morphological, histological, functional alternations examined using multiple approaches. The effector molecules hUSC-derived (hUSC-Exo) determined by differential expression analysis ovaries. target genes miRNA accessed transcriptome sequencing GCs, mechanisms further elucidated. Results remarkably inhibited promoted proliferation respectively. In addition, we observed that miR-27b-3p was highly expressed hUSC-Exo markedly suppressed GC specifically inhibiting SLC1A4, a serine transporter, which, turn, elevated concentration intracellular outflux cellular serine. More importantly, knockdown SLC1A4 simple supplementation GCs. Finally, demonstrated GCs essential reducing via elevating whereas protected against miR-27b-3p/SLC1A4/serine axis-mediated activation PI3K/AKT/mTOR signaling pathway. Conclusions Our suggests hUSC-based may provide an efficient approach prevention clinically. Graphical abstract
Язык: Английский
Процитировано
0
The Journal of Physical Chemistry A, Год журнала: 2025, Номер unknown
Опубликована: Март 19, 2025
Aliphatic hydroxyamino acids, namely, α-hydroxyglycine, α-hydroxyalanine, serine, threonine, and homoserine, were studied by quantum chemical calculations using two methods in water as a solvent. A hybrid variant of DFT-B3LYP was applied to optimize the geometry neutral molecules, molecular cations, anions for canonical zwitterionic form amino acids. In energy minimum, vibrational analysis applied, enabling evaluation thermodynamic functions (internal energy, enthalpy, entropy, Gibbs energy) individual species absolute oxidation reduction potentials redox couples. B3LYP preoptimized geometry, advanced DLPNO-CCSD(T) method include major part interelectron correlation energy. Calculated descriptors compared with previously molecules same method, whole set 17 acids processed statistical such cluster principal component analysis. correlate adiabatic ionization energies along straight line, analogously, calculated potential correlates electrophilicity index. The α-amino is systematically influenced (reduced) functional groups hydroxyl, methyl, ethyl, iso-propyl; it decreases series α-, β-, γ-, δ-amino
Язык: Английский
Процитировано
0
npj Aging, Год журнала: 2025, Номер 11(1)
Опубликована: Апрель 12, 2025
Increasing evidence suggests that frailty results from a complex age-associated metabolic decline. Here, we investigated the serum metabolomic profile of well-characterized cohort elderly subjects encompassing whole fit-to-frail continuum. Enrichment analyses revealed dysregulation amino acids and energy metabolism in both pre-frail frail participants. Remarkably, upregulated betaine levels emerged as specific biochemical signature females, holding promise for development novel targeted interventions.
Язык: Английский
Процитировано
0
Frontiers in Endocrinology, Год журнала: 2025, Номер 16
Опубликована: Май 14, 2025
Objective Ultra-high-performance liquid chromatography-time-of-flight mass spectrometry (UHPLC-TOF-MS) was employed to analyze serum metabolites and metabolic pathways associated with syndrome (MS) in the Yi Han populations of Yunnan. Methods Participants included individuals diagnosed MS healthy controls from Serum nontargeted amino acid-targeted metabolomics analyses were conducted identify differential (DEMs) pathogenesis these two ethnic groups. Results Nontargeted analysis revealed 2,762 DEMs group population, while 1,535 identified population [variable importance projection (VIP)>1, P <0.05]. Venn highlighted common unique between populations. KEGG pathway seven significantly enriched five group, primarily involving acid synthesis metabolism. To investigate role acids MS, levels 71 endogenous quantified. In 19 identified, related D-glutamine D-glutamate metabolism, as well cysteine methionine six significant enrichment sulfur valine, leucine, isoleucine biosynthesis. Conclusion Our study investigates differences through targeted approaches, identifying both especially disorders, including glycine, serine, threonine which may play critical roles regulating different dysfunctions worth further exploration pathogenesis, might provide insights for effective prevention treatment various
Язык: Английский
Процитировано
0
Metabolic Brain Disease, Год журнала: 2023, Номер 39(1), С. 249 - 251
Опубликована: Сен. 12, 2023
Язык: Английский
Процитировано
7
Foods, Год журнала: 2024, Номер 13(15), С. 2358 - 2358
Опубликована: Июль 26, 2024
The growing demand for alternative sources of non-animal proteins has stimulated research in this area. Mushrooms show potential the innovation plant-based food products. In study, aim was to develop prototype fish fillets analogues from Pleurotus ostreatus mushrooms applying enzymatic treatment (β-glucanase and transglutaminase-TG). A Plackett–Burman 20 experimental design used optimize forty variables. Oat flour (OF) exerted a positive effect on hardness gumminess texture parameters but negative cohesiveness resilience. Soy protein isolate (SPI) exhibited elasticity, chewiness, while acacia gum had After sensory analysis assay with 1% cassava starch, 5% OF, SPI, 0.1% transglutaminase (240 min/5 °C), coconut oil, soybean 0.2% sodium tripolyphosphate, 0.6% β-glucanase (80 °C/10 min) without inactivation found exhibit greater similarity fillet. classes hydrocarbons, alcohols aldehydes are predominant ones aromatic profile by chromatography electronic nose. It is concluded that mushroom-based analogue fillet can be prepared using TG.
Язык: Английский
Процитировано
2