A host‐derived chimeric peptide protects citrus against Huanglongbing without threatening the native microbial community of the phyllosphere DOI Creative Commons
Jeongyun Choi, Supratim Basu,

Abigail Thompson

и другие.

Journal of Sustainable Agriculture and Environment, Год журнала: 2023, Номер 2(4), С. 489 - 499

Опубликована: Ноя. 24, 2023

Abstract Introduction The application of host‐derived antibacterial peptides has been highlighted as a potential efficacious and safe tool for the treatment Huanglongbing (HLB), most devastating disease citrus. However, pathogenic bacteria such HLB‐causing Candidatus liberibacter asiaticus ( C Las) often develop resistance against host peptides. We showed that chimeras containing two different not only retain activity but also overcome bacterial enhance plant defence responses. Also, chimeric can have an off‐target impact on structure function plant‐associated microbiomes. there is lack understanding peptide therapy microbial in citrus phyllosphere while reducing Las titre. Here, we aim to evaluate efficacy (UGK17) reduce titre, inducing response impacting microbiome associated with phyllosphere. Material Method Leaf samples were collected from orange grapefruit trees Texas identified old young leaves according their maturity. three types based infection symptoms: healthy, symptomatic (infected typical symptoms), asymptomatic without symptoms). In planta assay was performed by dipping 0, 5 25 μM UGK17 solutions 48 h. quantifications titre pathogenesis‐related PR ) gene expression done quantitative polymerase chain reaction (qPCR) reverse transcription‐qPCR, respectively. Amplicon sequencing individual community structures. addition, ex assess effect growth isolates including Liberibacter crescens instead unculturable predominant Result reduced both leaves, regardless age leaves. augmented expression. assay, L. along four other strains belonging family Rhizobiaceae, significantly inhibited 74 unaffected. Additionally, no statistically significant changes treatment. Conclusion Our results suggest promising solution combat HLB targeting mainly Gram‐negative pathogens enhancing immune responses impairing indigenous

Язык: Английский

Groundbreaking Technologies and the Biocontrol of Fungal Vascular Plant Pathogens DOI Creative Commons
Carmen Gómez‐Lama Cabanás, Jesús Mercado‐Blanco

Journal of Fungi, Год журнала: 2025, Номер 11(1), С. 77 - 77

Опубликована: Янв. 18, 2025

This review delves into innovative technologies to improve the control of vascular fungal plant pathogens. It also briefly summarizes traditional biocontrol approaches manage them, addressing their limitations and emphasizing need develop more sustainable precise solutions. Powerful tools such as next-generation sequencing, meta-omics, microbiome engineering allow for targeted manipulation microbial communities enhance pathogen suppression. Microbiome-based include design synthetic consortia transplant entire or customized soil/plant microbiomes, potentially offering resilient adaptable strategies. Nanotechnology has advanced significantly, providing methods delivery biological agents (BCAs) compounds derived from them through different nanoparticles (NPs), including bacteriogenic, mycogenic, phytogenic, phycogenic, debris-derived ones acting carriers. The use biodegradable polymeric non-polymeric eco-friendly NPs, which enable controlled release antifungal while minimizing environmental impact, is explored. Furthermore, artificial intelligence machine learning can revolutionize crop protection early disease detection, prediction outbreaks, precision in BCA treatments. Other genome editing, RNA interference (RNAi), functional peptides efficacy against pathogenic fungi. Altogether, these provide a comprehensive framework management diseases, redefining modern agriculture.

Язык: Английский

Процитировано

1
How plants manage pathogen infection DOI Creative Commons

Yinan Jian,

Dianming Gong, Z. Wang

и другие.

EMBO Reports, Год журнала: 2023, Номер 25(1), С. 31 - 44

Опубликована: Дек. 19, 2023

Abstract To combat microbial pathogens, plants have evolved specific immune responses that can be divided into three essential steps: recognition by receptors, signal transduction within plant cells, and execution directly suppressing pathogens. During the past decades, many receptors signaling components their mode of action been revealed, markedly advancing our understanding first two steps. Activation results in physical chemical actions actually stop pathogen infection. Nevertheless, this third step immunity is under explored. In addition to plants, recent evidence suggests microbiota, which considered an additional layer system, also plays a critical role direct suppression. review, we summarize current how as well microbiota control growth behavior highlight outstanding questions need answered.

Язык: Английский

Процитировано

21
New insights into the antifungal and anti-mycotoxigenic potential of the TickCore3 peptide against Fusarium species causing Fusarium head blight DOI
Valentin Leannec-Rialland, Vessela Atanasova‐Pénichon, Alejandro Cabezas‐Cruz

и другие.

Journal of Plant Diseases and Protection, Год журнала: 2025, Номер 132(2)

Опубликована: Фев. 19, 2025

Язык: Английский

Процитировано

0
A plant peptide with dual activity against multidrug-resistant bacterial and fungal pathogens DOI Creative Commons
Xueyan Chen,

Meirong Song,

Lei Tian

и другие.

Science Advances, Год журнала: 2025, Номер 11(12)

Опубликована: Март 19, 2025

Multidrug-resistant (MDR) bacteria pose a major threat to public health, and additional sources of antibacterial candidates are urgently needed. Noncanonical peptides (NCPs), derived from noncanonical small open reading frames, represent biological molecules with important roles in biology. However, the activity NCPs remains largely unknown. Here, we discovered plant-derived peptide (NCBP1) against both Gram-positive Gram-negative bacteria. NCBP1 is composed 11 amino acid residues cationic surface potential favorable safety stability. Mechanistic studies revealed that displayed by targeting phosphatidylglycerol cardiolipin bacterial membrane, resulting membrane damage dysfunction. Notably, showed promising efficacy mice. Furthermore, effectively inhibited growth plant fungal pathogens enhanced disease resistance maize. Our results demonstrate unexplored antimicrobial provide an accessible source for discovery substances MDR pathogens.

Язык: Английский

Процитировано

0
Whole genome-sequence analysis of Bacillus subtilis strain KC14-1 with broad-spectrum antifungal activity DOI Creative Commons
Xiaowei Li, Yahan Chen, Shunyi Yang

и другие.

BMC Genomics, Год журнала: 2025, Номер 26(1)

Опубликована: Март 31, 2025

Bacillus is used as a biological control agent in agricultural production. The main mechanisms responsible for its biocontrol activity encompass the generation of various antifungal active substances during life activities, competition, antagonism with pathogens, promotion growth, and induction plant resistance, enhancing inhibition pathogenic fungi. has high potential become research hotspot. It was found that strain KC14-1 had significant inhibitory effects on Fusarium fujikuroi, Rhizoclonia solani, Alternaria oxysporum, Valsa mali. Based morphological observations, physiological biochemical determinations, 16 S rRNA, gyrA, gyrB gene sequencing, identified subtilis. Whole genome sequencing results showed composed ring chromosome 3,908,079 bp size, GC content 43.82% 3,895 coding genes. Anti-SMASH predicted contained nine clusters synthesised antibacterial substances. homology between fengycin, bacillibactin, pulcherriminic acid, subtilosin A, bacilysin 100%. subtilis determined through whole-genome analysis. Our study provides solid foundation developing utilising this strain.

Язык: Английский

Процитировано

0
Plant Fungal Diseases and Crop Protection DOI Creative Commons
Ofir Degani

Journal of Fungi, Год журнала: 2025, Номер 11(4), С. 274 - 274

Опубликована: Апрель 1, 2025

Fungi represent the largest group of plant pathogens, infecting their hosts via leaves, seeds, and roots [...]

Язык: Английский

Процитировано

0
Synthetic peptides bioactive against phytopathogens have lower impact on some beneficial bacteria: An assessment of peptides biosafety in agriculture DOI
Emil Gimranov, João Vasco Santos, Laura Regalado

и другие.

Journal of Environmental Management, Год журнала: 2025, Номер 374, С. 123942 - 123942

Опубликована: Янв. 8, 2025

Язык: Английский

Процитировано

0
Pantoea–Bacillus as a Composite Microbial Community: Inhibition and Potential Mechanism Against Potato Anthracnose Disease DOI Creative Commons
Haojie Zhang,

Huiqin Shi,

M. X. Luo

и другие.

Journal of Fungi, Год журнала: 2025, Номер 11(2), С. 121 - 121

Опубликована: Фев. 5, 2025

The potato (Solanum tuberosum), an important component of global food security, often faces threats from various diseases during its growth process, especially anthracnose (Colletotrichum coccodes), which severely affects crop yield and quality. In this study, we successfully isolated identified two bacteria with potential for biological control, (Pantoea agglomerans) (Bacillus subtilis). experimental results indicate that the bacterial suspensions strains JZ-1-1-1 JZ-2-2-2 had a significant inhibitory effect on pathogen ZL-7, inhibition rate reaching as high 55.21%. was 53.48%. When these were mixed at 4:6 ratio, pathogenic even more significant, 68.58% inhibition. addition, composite microbial community produced biofilms their gradually increasing within 24 h showing slight decrease after 72 h. efficacy test further indicated suspension highly effective in controlling spread lesions, 81.40%. analysis defense enzyme activity, peroxidase (POD) superoxide dismutase (SOD) levels peaked day seven, while significantly reduced malondialdehyde (MDA) polyphenol oxidase (PPO) activity. Quantitative real-time PCR confirmed effectively colonized surface tubers. summary, study provides theoretical basis practical guidance application methods prevention control anthracnose.

Язык: Английский

Процитировано

0
Erucamide: A special biological control agent targeting T3SS for plant bacterial diseases DOI Creative Commons

Longzhu Bao,

Chang Min, Shaoyong Ke

и другие.

Advanced Agrochem, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Host Defense Peptide-Mimicking Poly(2-oxazoline)s Displaying Potent Activities toward Phytopathogens to Alleviate Antimicrobial Resistance in Agriculture DOI
Sheng Chen, Min Zhou, Ximian Xiao

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 26, 2025

Given the limited types of agricultural bactericides and rapid emergence antimicrobial resistance, bacterial plant diseases pose a serious threat to production, which calls for effective agents with low propensity resistance. Host defense peptides (HDPs) have drawn significant attention their broad-spectrum activity. In this study, we found that HPD-mimicking poly(2-oxazoline) Gly-POX20 exhibits potent activity against phytopathogens, superior antibacterial selectivity proteolytic stability compared natural HDP melittin. Compared commonly used bactericides, displays more efficient antibiofilm lower resistance than does antibiotic streptomycin, likely due its mechanism, involves DNA interaction generating lethal doses ROS. vivo studies reveal is in preventing treating phytopathogens without observable damage tissues, suggesting could be promising bactericide applications.

Язык: Английский

Процитировано

0