Groundbreaking Technologies and the Biocontrol of Fungal Vascular Plant Pathogens

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(1), P. 77 - 77

Published: Jan. 18, 2025

This review delves into innovative technologies to improve the control of vascular fungal plant pathogens. It also briefly summarizes traditional biocontrol approaches manage them, addressing their limitations and emphasizing need develop more sustainable precise solutions. Powerful tools such as next-generation sequencing, meta-omics, microbiome engineering allow for targeted manipulation microbial communities enhance pathogen suppression. Microbiome-based include design synthetic consortia transplant entire or customized soil/plant microbiomes, potentially offering resilient adaptable strategies. Nanotechnology has advanced significantly, providing methods delivery biological agents (BCAs) compounds derived from them through different nanoparticles (NPs), including bacteriogenic, mycogenic, phytogenic, phycogenic, debris-derived ones acting carriers. The use biodegradable polymeric non-polymeric eco-friendly NPs, which enable controlled release antifungal while minimizing environmental impact, is explored. Furthermore, artificial intelligence machine learning can revolutionize crop protection early disease detection, prediction outbreaks, precision in BCA treatments. Other genome editing, RNA interference (RNAi), functional peptides efficacy against pathogenic fungi. Altogether, these provide a comprehensive framework management diseases, redefining modern agriculture.

Language: Английский

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Whole genome-sequence analysis of Bacillus subtilis strain KC14-1 with broad-spectrum antifungal activity

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: March 31, 2025

Bacillus is used as a biological control agent in agricultural production. The main mechanisms responsible for its biocontrol activity encompass the generation of various antifungal active substances during life activities, competition, antagonism with pathogens, promotion growth, and induction plant resistance, enhancing inhibition pathogenic fungi. has high potential become research hotspot. It was found that strain KC14-1 had significant inhibitory effects on Fusarium fujikuroi, Rhizoclonia solani, Alternaria oxysporum, Valsa mali. Based morphological observations, physiological biochemical determinations, 16 S rRNA, gyrA, gyrB gene sequencing, identified subtilis. Whole genome sequencing results showed composed ring chromosome 3,908,079 bp size, GC content 43.82% 3,895 coding genes. Anti-SMASH predicted contained nine clusters synthesised antibacterial substances. homology between fengycin, bacillibactin, pulcherriminic acid, subtilosin A, bacilysin 100%. subtilis determined through whole-genome analysis. Our study provides solid foundation developing utilising this strain.

Language: Английский

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How plants manage pathogen infection

EMBO Reports, Journal Year: 2023, Volume and Issue: 25(1), P. 31 - 44

Published: Dec. 19, 2023

Abstract To combat microbial pathogens, plants have evolved specific immune responses that can be divided into three essential steps: recognition by receptors, signal transduction within plant cells, and execution directly suppressing pathogens. During the past decades, many receptors signaling components their mode of action been revealed, markedly advancing our understanding first two steps. Activation results in physical chemical actions actually stop pathogen infection. Nevertheless, this third step immunity is under explored. In addition to plants, recent evidence suggests microbiota, which considered an additional layer system, also plays a critical role direct suppression. review, we summarize current how as well microbiota control growth behavior highlight outstanding questions need answered.

Language: Английский

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New Tools for the Management of Fungal Pathogens in Extensive Cropping Systems for Friendly Environments

Critical Reviews in Plant Sciences, Journal Year: 2023, Volume and Issue: 43(2), P. 63 - 93

Published: Oct. 20, 2023

AbstractCrop production plays a critical role in global food security, with key commodities such as corn, wheat, soybean, and rice ranking among the most widely cultivated crops. These major crops are predominantly grown within extensive cropping systems. However, these systems threatened by fungal diseases, which may cause substantial yield reductions. The adopted strategy to manage pathogens extensively worldwide is chemical control. Nevertheless, this has multiple drawbacks potential hazards, including pathogen resistance, environmental contamination, negative effects on human health other organisms. As logical result, over last decades, conventional agricultural have been questioned transition toward more sustainable methods emerged. new productive paradigm emphasizes adoption of eco-friendly approaches disease management, biofungicides biostimulants tools gaining popularity. establishing regulatory framework for different countries proven challenging due lack harmonization. primary objective review gather dispersed information technologies (either available market or being studied) applicable generated latest scientific advances. Additionally, seeks contribute clarifying categorization (biostimulants, biofungicides, plant defense inducers, gene editing, RNAi, nanotechnology, physical treatment) enhance their understanding critically assess potentials, challenges, future perspectives. Furthermore, aims identify successfully implemented horticulture intensive but not yet practically applied crops, pave way advances adaptations suit practices. Finally, presents practical management model that incorporates address wheat.Keywords: Extensive systemsintegrated managementnew toolsbiostimulantsbiofungicidesplant resistance inducersdual-effect Disclosure statementThe authors declare no conflict interest.Data availability statementData sharing article data were created analyzed study.Additional informationFundingThe publication was part collaborative work co-financed University Buenos Aires (UBACYT 20020220100114BA), Argentina, Polish National Agency Academic - NAWA (BPI/PST/2021/1/00034).

Language: Английский

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Synthetic peptides bioactive against phytopathogens have lower impact on some beneficial bacteria: An assessment of peptides biosafety in agriculture

Journal of Environmental Management, Journal Year: 2025, Volume and Issue: 374, P. 123942 - 123942

Published: Jan. 8, 2025

Language: Английский

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Pantoea–Bacillus as a Composite Microbial Community: Inhibition and Potential Mechanism Against Potato Anthracnose Disease

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(2), P. 121 - 121

Published: Feb. 5, 2025

The potato (Solanum tuberosum), an important component of global food security, often faces threats from various diseases during its growth process, especially anthracnose (Colletotrichum coccodes), which severely affects crop yield and quality. In this study, we successfully isolated identified two bacteria with potential for biological control, (Pantoea agglomerans) (Bacillus subtilis). experimental results indicate that the bacterial suspensions strains JZ-1-1-1 JZ-2-2-2 had a significant inhibitory effect on pathogen ZL-7, inhibition rate reaching as high 55.21%. was 53.48%. When these were mixed at 4:6 ratio, pathogenic even more significant, 68.58% inhibition. addition, composite microbial community produced biofilms their gradually increasing within 24 h showing slight decrease after 72 h. efficacy test further indicated suspension highly effective in controlling spread lesions, 81.40%. analysis defense enzyme activity, peroxidase (POD) superoxide dismutase (SOD) levels peaked day seven, while significantly reduced malondialdehyde (MDA) polyphenol oxidase (PPO) activity. Quantitative real-time PCR confirmed effectively colonized surface tubers. summary, study provides theoretical basis practical guidance application methods prevention control anthracnose.

Language: Английский

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New insights into the antifungal and anti-mycotoxigenic potential of the TickCore3 peptide against Fusarium species causing Fusarium head blight

Journal of Plant Diseases and Protection, Journal Year: 2025, Volume and Issue: 132(2)

Published: Feb. 19, 2025

Language: Английский

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A plant peptide with dual activity against multidrug-resistant bacterial and fungal pathogens

Science Advances, Journal Year: 2025, Volume and Issue: 11(12)

Published: March 19, 2025

Multidrug-resistant (MDR) bacteria pose a major threat to public health, and additional sources of antibacterial candidates are urgently needed. Noncanonical peptides (NCPs), derived from noncanonical small open reading frames, represent biological molecules with important roles in biology. However, the activity NCPs remains largely unknown. Here, we discovered plant-derived peptide (NCBP1) against both Gram-positive Gram-negative bacteria. NCBP1 is composed 11 amino acid residues cationic surface potential favorable safety stability. Mechanistic studies revealed that displayed by targeting phosphatidylglycerol cardiolipin bacterial membrane, resulting membrane damage dysfunction. Notably, showed promising efficacy mice. Furthermore, effectively inhibited growth plant fungal pathogens enhanced disease resistance maize. Our results demonstrate unexplored antimicrobial provide an accessible source for discovery substances MDR pathogens.

Language: Английский

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Erucamide: A special biological control agent targeting T3SS for plant bacterial diseases

Advanced Agrochem, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

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Host Defense Peptide-Mimicking Poly(2-oxazoline)s Displaying Potent Activities toward Phytopathogens to Alleviate Antimicrobial Resistance in Agriculture

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Given the limited types of agricultural bactericides and rapid emergence antimicrobial resistance, bacterial plant diseases pose a serious threat to production, which calls for effective agents with low propensity resistance. Host defense peptides (HDPs) have drawn significant attention their broad-spectrum activity. In this study, we found that HPD-mimicking poly(2-oxazoline) Gly-POX20 exhibits potent activity against phytopathogens, superior antibacterial selectivity proteolytic stability compared natural HDP melittin. Compared commonly used bactericides, displays more efficient antibiofilm lower resistance than does antibiotic streptomycin, likely due its mechanism, involves DNA interaction generating lethal doses ROS. vivo studies reveal is in preventing treating phytopathogens without observable damage tissues, suggesting could be promising bactericide applications.

Language: Английский

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