International Journal of Biological Macromolecules, Год журнала: 2024, Номер unknown, С. 139057 - 139057
Опубликована: Дек. 1, 2024
Язык: Английский
Frontiers in Physiology, Год журнала: 2025, Номер 16
Опубликована: Март 27, 2025
Introduction: Hypertension is a significant risk factor for kidney disease. Aerobic exercise has demonstrated positive effects in managing hypertensive nephropathy. However, the impact of on nephropathy remains contentious due to variations protocols. This study aimed compare moderate-intensity continuous training (MICT) and high-intensity interval (HIIT) renal fibrosis spontaneously rats (SHRs). Methods: SHRs underwent 10-week treadmill with (HIIT). The blood pressure was measured following conclusion final session. function, levels HIF-1α, fibrosis, autophagy were evaluated by immunostaining western blot rat kidneys. AKT/mTOR signaling pathway also investigated. In vitro, we treated angiotensin II-induced HK-2 cells inhibited or overexpressed HIF-1α tested changes blot. Following treatment lysosomal inhibitors (chloroquine), expression further Results: Our findings indicated that MICT improved function through downregulation autophagy, whereas HIIT did not lead improvement. Additionally, inhibition attenuates Ang cells. overexpression had opposite effect. CQ alleviates fibrosis. Conclusion: These elucidated potential ameliorate caused hypertension targeting HIF-1α-regulated autophagy.
Язык: Английский
Процитировано
0
Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 12
Опубликована: Дек. 11, 2024
Chronic kidney disease (CKD) represents a significant global public health burden, affecting over 10% of the world’s population. Its high morbidity, multifactorial complications, and substantial mortality impose burdens on healthcare systems patients, necessitating considerable investment in resources. Renal fibrosis (RF) is key pathological feature driver CKD progression. Extensive research indicates that autophagy participates complete pathogenesis RF. Under physiological conditions, essential for maintaining renal cellular homeostasis. However, under perhaps aberrant sustained activation contributes to oxidative stress, apoptosis, inflammation, etc. Ultimately, they accelerate development The role RF currently controversial. This review investigates molecular mechanisms by which intrinsic cell across diverse models, suggesting its associated regulatory pathways represent potential diagnostic therapeutic targets CKD.
Язык: Английский
Процитировано
1
Biology, Год журнала: 2024, Номер 13(7), С. 540 - 540
Опубликована: Июль 18, 2024
Foreign body response (FBR) is a universal reaction to implanted biomaterial that can affect the function and longevity of implant. A few studies have attempted identify targets for treating FBR through use single-cell RNA sequencing (scRNA-seq), though generalizability these findings from an individual study may be limited. In our study, we perform meta-analysis scRNA-seq data all available mouse integrate gene signatures specific across different models anatomic locations. We subclusters fibroblasts macrophages emerge in foreign bodies characterize their signaling pathways, ontology terms, downstream mediators. The fibroblast subpopulations enriched setting demonstrated significant interactions transforming growth factor-beta (TGF-β) pathway, with known pro-fibrotic mediators identified as top expressed genes FBR-derived fibroblasts. contrast, FBR-enriched macrophage highly pro-inflammatory tumor necrosis factor (TNF) signaling. Cell–cell were additionally interrogated using CellChat, identification key between FBR. By combining multiple datasets, identifies common cell-specific reactions, providing potential therapeutic patients requiring medical implants myriad devices indications.
Язык: Английский
Процитировано
0
Molecular Therapy, Год журнала: 2024, Номер 33(1), С. 249 - 262
Опубликована: Сен. 17, 2024
Язык: Английский
Процитировано
0
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 13013 - 13013
Опубликована: Дек. 3, 2024
Wound healing as a result of skin injury involves series dynamic physiological processes, leading to wound closure, re-epithelialization, and the remodeling extracellular matrix (ECM). The primary scar formed by new ECM never fully regains original tissue’s strength or flexibility. Moreover, in some cases, due dysregulated fibroblast activity, proliferation, differentiation, normal scarring can be replaced pathological fibrotic tissue, hypertrophic scars keloids. These disorders cause significant physical impairment psychological stress represent challenges medical management wound-healing process. present study aimed investigate therapeutic effects exogenously applied carbon dioxide (CO2) on behavior, focusing viability, migration, differentiation myofibroblasts. We found that CO2 exposure for up 60 min did not significantly affect apoptosis rate, proliferation migration capacities. However, notable finding was reduction α-smooth muscle actin (α-SMA) protein expression, indicative myofibroblast inhibition, following exposure. This effect specific concentration well time-dependent, with longer durations greater reductions α-SMA expression. Furthermore, inhibition correlated statistically reduced glycolytic mitochondrial energy metabolism, result, ATP synthesis rate. very noticeable decrease cellular levels seemed could observed control cultures using nitrogen (N2)-saturated solutions, indicating unique hypoxia-independent metabolism. findings suggest may possess differentiation-reducing properties modulating fibroblast’s metabolism offer options prevention keloid development.
Язык: Английский
Процитировано
0
International Journal of Biological Macromolecules, Год журнала: 2024, Номер unknown, С. 139057 - 139057
Опубликована: Дек. 1, 2024
Язык: Английский
Процитировано
0