Network pharmacology and molecular docking to explore the potential mechanism of Chlorogenic acid in combined septic acute liver injury DOI Creative Commons

Shangping Fang,

Hui Su,

Jiameng Liu

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 22, 2024

Abstract Objective To investigate the biological activities and mechanisms of chlorogenic acid (CGA) in treating septic acute liver injury (SALI) using network pharmacology, molecular docking, vivo studies. Methods Potential targets related to both were searched from public databases. Protein-protein interaction (PPI), Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses conducted. Molecular docking was performed predict binding affinity between active compounds core targets. Finally, vitro experiments carried out for further validation. Results A total 60 common identified acid, among which 10 shared screened Cytoscape. results indicated that these had good activity with acid. In SALI mouse model, demonstrated significant protective effects on anti-inflammatory properties, acting through TLR4/NF-κB pathway. Conclusion CGA not only improves pathological damage but also exerts its potentially multiple pathways including TLR4.

Язык: Английский

Enzymatic depletion of circulating glutamine is immunosuppressive in cancers DOI Creative Commons
Monish Kumar,

Ankita Leekha,

Suman Nandy

и другие.

iScience, Год журнала: 2024, Номер 27(6), С. 109817 - 109817

Опубликована: Апрель 26, 2024

Highlights•H. pylori γ-glutamyl transferase (GGT) depletes glutamine for up to 72 h in mice•PEG-GGT treatment increases infiltration of myeloid-derived suppressive cells tumors•Across diverse cancers, TCGA analyses revealed depletion is not beneficialSummaryAlthough addiction cancer extensively reported, there controversy on the impact metabolism immune within tumor microenvironment (TME). To address role extracellular glutamine, we enzymatically depleted circulating using PEGylated Helicobacter gamma-glutamyl (PEG-GGT) syngeneic mouse models breast and colon cancers. PEG-GGT inhibits growth vitro, but vivo it suppressor (MDSCs) has no significant growth. By deriving a signature, analyze human cancers illustrate that associated with favorable clinical outcomes correlates accumulation MDSC. Broadly, our results help clarify integrated TME advance as an enzymatic tool systemic selective (no asparaginase activity) live animals.Graphical abstract

Язык: Английский

Процитировано

5
Network pharmacology and molecular docking to explore the potential mechanism of chlorogenic acid in septic acute liver injury and experimental validation of TLR4/NF-κB pathway in vivo DOI Creative Commons

Shangping Fang,

Hui Su,

Jiameng Liu

и другие.

Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 2, 2025

The objective of this study was to investigate the biological activities and mechanisms chlorogenic acid (CGA) in treatment septic acute liver injury (SALI) based on network pharmacology, molecular docking, vivo studies, other techniques. Chlorogenic potential related targets were searched from public databases. Then, protein–protein interaction (PPI), Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses conducted. Subsequently, docking performed predict binding active compound core target. Finally, experiments carried out for further validation. A total 60 common identified between acid, among which 9 (EGFR, ESR1, GSK3B, PTGS2, TLR4, PPARA, HSP90AA1, ACE, MMP9) screened with Cytoscape. Molecular indicated that these had good activity (− 7.2, − 6.8, 7.7, 8.7, 6.1, 7.3, 8.4, 8.6 kcal/mol respectively). In SALI mouse model, can improve pathological damage apoptosis cells, anti-inflammatory properties significantly by TLR4/NF-κB (all P < 0.05). regulatory CGA revealed, effect verified, could be associated pathway.

Язык: Английский

Процитировано

0
G protein-coupled purinergic P2Y receptors in infectious diseases DOI
M. Lucília Carvalho, Vinícius Santos Alves, Robson Coutinho‐Silva

и другие.

Pharmacology & Therapeutics, Год журнала: 2025, Номер unknown, С. 108796 - 108796

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
JNK inhibition mitigates sepsis-associated encephalopathy via attenuation of neuroinflammation, oxidative stress and apoptosis DOI

Riya Gagnani,

Harshita Singh, Manisha Suri

и другие.

Metabolic Brain Disease, Год журнала: 2025, Номер 40(3)

Опубликована: Март 13, 2025

Язык: Английский

Процитировано

0
The Predictive Role of FGF21 in Acute Liver Injury Caused by Bacterial Infectious Diseases in Critical Care: A Retrospective Cohort Study DOI Creative Commons
Zhijun Zhang, Yuan Li, Junqing Zhang

и другие.

Journal of Inflammation Research, Год журнала: 2025, Номер Volume 18, С. 6795 - 6806

Опубликована: Май 1, 2025

Acute liver injury (ALI) is a common complication in critically ill patients and has been strongly associated with adverse clinical outcomes. Early detection timely management of ALI these are crucial for implementing effective therapeutic strategies to prevent disease progression improve patient In this study, 112 bacterial infectious diseases were categorized into two groups based on the presence or absence within 24 hours intensive care unit (ICU) admission. Serum concentrations fibroblast growth factor 21 (FGF21), interleukin(IL)-6, IL-22, IL-10, enzymes, hypersensitive C-reactive protein (hs-CRP), D-Dimer (D2) measured ICU Demographic data recorded. Logistic regression analysis was performed identify potentially predictive biomarkers ALI. Receiver operating characteristic (ROC) curve employed determine optimal model predicting patients. Patients group exhibited significantly higher serum levels IL-6, FGF21, lactic acid, procalcitonin, D2, APACHE II scores, shorter survival time 28-day mortality compared those non-ALI group. indicated that age, gender, plasma direct bilirubin (DBIL), IL-22 FGF21 valuable predictors among ROC revealed achieved high area under 0.885, demonstrating excellent discriminatory ability. Elevated early stages critical illness may represent promising novel biomarker

Язык: Английский

Процитировано

0
Joannesia princeps Vell. inhibits purinergic P2Y receptors and presents antinociceptive and anti-inflammatory effects DOI
Natiele Carla da Silva Ferreira,

Mariana Chiste Pontes,

Ana Luíza Rangel Bérenger

и другие.

Biomedicine & Pharmacotherapy, Год журнала: 2025, Номер 188, С. 118195 - 118195

Опубликована: Май 29, 2025

Язык: Английский

Процитировано

0
METFORMIN MITIGATES SEPSIS-ASSOCIATED PULMONARY FIBROSIS BY PROMOTING AMPK ACTIVATION AND INHIBITING HIF-1α–INDUCED AEROBIC GLYCOLYSIS DOI

Han Zhong,

Ri Tang,

Jinhua Feng

и другие.

Shock, Год журнала: 2023, Номер 61(2), С. 283 - 293

Опубликована: Ноя. 15, 2023

ABSTRACT Recent research has revealed that aerobic glycolysis a strong correlation with sepsis-associated pulmonary fibrosis (PF). However, at present, the mechanism and pathogenesis remain unclear. We aimed to test hypothesis adenosine monophosphate-activated protein kinase (AMPK) activation suppression of hypoxia-inducible factor 1α (HIF-1α)–induced play central role in septic fibrogenesis. Cellular experiments demonstrated lipopolysaccharide increased fibroblast through AMPK inactivation, HIF-1α induction, alongside an augmentation glycolysis. By contrast, effects were reversed by or inhibition. In addition, pretreatment metformin, which is activator, suppresses expression alleviates PF associated sepsis, caused glycolysis, mice. Hypoxia-inducible knockdown similar protective vivo . Our implies targeting HIF-1α–induced metformin might be practical useful therapeutic alternative for PF.

Язык: Английский

Процитировано

7
A focus on c-Jun-N-terminal kinase signaling in sepsis-associated multiple organ dysfunction: Mechanisms and therapeutic strategies DOI

Riya Gagnani,

Mugdha Srivastava, Manisha Suri

и другие.

International Immunopharmacology, Год журнала: 2024, Номер 143, С. 113552 - 113552

Опубликована: Ноя. 15, 2024

Язык: Английский

Процитировано

2
The P2Y2 receptor as a sensor of nucleotides and cell recruitment during inflammatory processes of the liver DOI
Vinícius Santos Alves, Fabiana Cristina‐Rodrigues, Robson Coutinho‐Silva

и другие.

Purinergic Signalling, Год журнала: 2024, Номер 20(5), С. 465 - 467

Опубликована: Апрель 17, 2024

Язык: Английский

Процитировано

0
Network pharmacology and molecular docking to explore the potential mechanism of Chlorogenic acid in combined septic acute liver injury DOI Creative Commons

Shangping Fang,

Hui Su,

Jiameng Liu

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 22, 2024

Abstract Objective To investigate the biological activities and mechanisms of chlorogenic acid (CGA) in treating septic acute liver injury (SALI) using network pharmacology, molecular docking, vivo studies. Methods Potential targets related to both were searched from public databases. Protein-protein interaction (PPI), Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses conducted. Molecular docking was performed predict binding affinity between active compounds core targets. Finally, vitro experiments carried out for further validation. Results A total 60 common identified acid, among which 10 shared screened Cytoscape. results indicated that these had good activity with acid. In SALI mouse model, demonstrated significant protective effects on anti-inflammatory properties, acting through TLR4/NF-κB pathway. Conclusion CGA not only improves pathological damage but also exerts its potentially multiple pathways including TLR4.

Язык: Английский

Процитировано

0