Network pharmacology and molecular docking to explore the potential mechanism of Chlorogenic acid in combined septic acute liver injury DOI Creative Commons

Shangping Fang,

Hui Su,

Jiameng Liu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 22, 2024

Abstract Objective To investigate the biological activities and mechanisms of chlorogenic acid (CGA) in treating septic acute liver injury (SALI) using network pharmacology, molecular docking, vivo studies. Methods Potential targets related to both were searched from public databases. Protein-protein interaction (PPI), Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses conducted. Molecular docking was performed predict binding affinity between active compounds core targets. Finally, vitro experiments carried out for further validation. Results A total 60 common identified acid, among which 10 shared screened Cytoscape. results indicated that these had good activity with acid. In SALI mouse model, demonstrated significant protective effects on anti-inflammatory properties, acting through TLR4/NF-κB pathway. Conclusion CGA not only improves pathological damage but also exerts its potentially multiple pathways including TLR4.

Language: Английский

Enzymatic depletion of circulating glutamine is immunosuppressive in cancers DOI Creative Commons
Monish Kumar,

Ankita Leekha,

Suman Nandy

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(6), P. 109817 - 109817

Published: April 26, 2024

Highlights•H. pylori γ-glutamyl transferase (GGT) depletes glutamine for up to 72 h in mice•PEG-GGT treatment increases infiltration of myeloid-derived suppressive cells tumors•Across diverse cancers, TCGA analyses revealed depletion is not beneficialSummaryAlthough addiction cancer extensively reported, there controversy on the impact metabolism immune within tumor microenvironment (TME). To address role extracellular glutamine, we enzymatically depleted circulating using PEGylated Helicobacter gamma-glutamyl (PEG-GGT) syngeneic mouse models breast and colon cancers. PEG-GGT inhibits growth vitro, but vivo it suppressor (MDSCs) has no significant growth. By deriving a signature, analyze human cancers illustrate that associated with favorable clinical outcomes correlates accumulation MDSC. Broadly, our results help clarify integrated TME advance as an enzymatic tool systemic selective (no asparaginase activity) live animals.Graphical abstract

Language: Английский

Citations

5
Network pharmacology and molecular docking to explore the potential mechanism of chlorogenic acid in septic acute liver injury and experimental validation of TLR4/NF-κB pathway in vivo DOI Creative Commons

Shangping Fang,

Hui Su,

Jiameng Liu

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

The objective of this study was to investigate the biological activities and mechanisms chlorogenic acid (CGA) in treatment septic acute liver injury (SALI) based on network pharmacology, molecular docking, vivo studies, other techniques. Chlorogenic potential related targets were searched from public databases. Then, protein–protein interaction (PPI), Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses conducted. Subsequently, docking performed predict binding active compound core target. Finally, experiments carried out for further validation. A total 60 common identified between acid, among which 9 (EGFR, ESR1, GSK3B, PTGS2, TLR4, PPARA, HSP90AA1, ACE, MMP9) screened with Cytoscape. Molecular indicated that these had good activity (− 7.2, − 6.8, 7.7, 8.7, 6.1, 7.3, 8.4, 8.6 kcal/mol respectively). In SALI mouse model, can improve pathological damage apoptosis cells, anti-inflammatory properties significantly by TLR4/NF-κB (all P < 0.05). regulatory CGA revealed, effect verified, could be associated pathway.

Language: Английский

Citations

0
G protein-coupled purinergic P2Y receptors in infectious diseases DOI
M. Lucília Carvalho, Vinícius Santos Alves, Robson Coutinho‐Silva

et al.

Pharmacology & Therapeutics, Journal Year: 2025, Volume and Issue: unknown, P. 108796 - 108796

Published: Jan. 1, 2025

Language: Английский

Citations

0
JNK inhibition mitigates sepsis-associated encephalopathy via attenuation of neuroinflammation, oxidative stress and apoptosis DOI

Riya Gagnani,

Harshita Singh, Manisha Suri

et al.

Metabolic Brain Disease, Journal Year: 2025, Volume and Issue: 40(3)

Published: March 13, 2025

Language: Английский

Citations

0
A focus on c-Jun-N-terminal kinase signaling in sepsis-associated multiple organ dysfunction: Mechanisms and therapeutic strategies DOI

Riya Gagnani,

Mugdha Srivastava, Manisha Suri

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 143, P. 113552 - 113552

Published: Nov. 15, 2024

Language: Английский

Citations

2
METFORMIN MITIGATES SEPSIS-ASSOCIATED PULMONARY FIBROSIS BY PROMOTING AMPK ACTIVATION AND INHIBITING HIF-1α–INDUCED AEROBIC GLYCOLYSIS DOI

Han Zhong,

Ri Tang,

Jinhua Feng

et al.

Shock, Journal Year: 2023, Volume and Issue: 61(2), P. 283 - 293

Published: Nov. 15, 2023

ABSTRACT Recent research has revealed that aerobic glycolysis a strong correlation with sepsis-associated pulmonary fibrosis (PF). However, at present, the mechanism and pathogenesis remain unclear. We aimed to test hypothesis adenosine monophosphate-activated protein kinase (AMPK) activation suppression of hypoxia-inducible factor 1α (HIF-1α)–induced play central role in septic fibrogenesis. Cellular experiments demonstrated lipopolysaccharide increased fibroblast through AMPK inactivation, HIF-1α induction, alongside an augmentation glycolysis. By contrast, effects were reversed by or inhibition. In addition, pretreatment metformin, which is activator, suppresses expression alleviates PF associated sepsis, caused glycolysis, mice. Hypoxia-inducible knockdown similar protective vivo . Our implies targeting HIF-1α–induced metformin might be practical useful therapeutic alternative for PF.

Language: Английский

Citations

6
The P2Y2 receptor as a sensor of nucleotides and cell recruitment during inflammatory processes of the liver DOI
Vinícius Santos Alves, Fabiana Cristina‐Rodrigues, Robson Coutinho‐Silva

et al.

Purinergic Signalling, Journal Year: 2024, Volume and Issue: 20(5), P. 465 - 467

Published: April 17, 2024

Language: Английский

Citations

0
Network pharmacology and molecular docking to explore the potential mechanism of Chlorogenic acid in combined septic acute liver injury DOI Creative Commons

Shangping Fang,

Hui Su,

Jiameng Liu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 22, 2024

Abstract Objective To investigate the biological activities and mechanisms of chlorogenic acid (CGA) in treating septic acute liver injury (SALI) using network pharmacology, molecular docking, vivo studies. Methods Potential targets related to both were searched from public databases. Protein-protein interaction (PPI), Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses conducted. Molecular docking was performed predict binding affinity between active compounds core targets. Finally, vitro experiments carried out for further validation. Results A total 60 common identified acid, among which 10 shared screened Cytoscape. results indicated that these had good activity with acid. In SALI mouse model, demonstrated significant protective effects on anti-inflammatory properties, acting through TLR4/NF-κB pathway. Conclusion CGA not only improves pathological damage but also exerts its potentially multiple pathways including TLR4.

Language: Английский

Citations

0