Labeling of active neural circuits in vivo with designed calcium integrators

Science, Год журнала: 2015, Номер 347(6223), С. 755 - 760

Опубликована: Фев. 12, 2015

The identification of active neurons and circuits in vivo is a fundamental challenge understanding the neural basis behavior. Genetically encoded calcium (Ca(2+)) indicators (GECIs) enable quantitative monitoring cellular-resolution activity during However, such require online within limited field view. Alternatively, post hoc staining immediate early genes (IEGs) indicates highly cells entire brain, albeit with poor temporal resolution. We designed fluorescent sensor, CaMPARI, that combines genetic targetability link to GECIs permanent, large-scale labeling IEGs, allowing temporally precise "activity snapshot" large tissue volume. CaMPARI undergoes efficient irreversible green-to-red conversion only when elevated intracellular Ca(2+) experimenter-controlled illumination coincide. demonstrate utility freely moving larvae zebrafish flies, head-fixed mice adult flies.

Язык: Английский

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Circadian neuron feedback controls the Drosophila sleep–activity profile

Nature, Год журнала: 2016, Номер 536(7616), С. 292 - 297

Опубликована: Авг. 1, 2016

Язык: Английский

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Distinct Dopamine Receptor Pathways Underlie the Temporal Sensitivity of Associative Learning

Cell, Год журнала: 2019, Номер 178(1), С. 60 - 75.e19

Опубликована: Июнь 1, 2019

Язык: Английский

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The Drosophila Circadian Clock Is a Variably Coupled Network of Multiple Peptidergic Units

Science, Год журнала: 2014, Номер 343(6178), С. 1516 - 1520

Опубликована: Март 27, 2014

Circadian Rhythms rhythms in the fruit fly Drosophila are driven by neurons brain. Yao and Shafer (p. 1516 ) analyzed different sets of that can drive circadian rhythms. Manipulating period each set separately revealed when various clock signals were fairly consistent, showed a robust rhythm. But seriously out sync with one another, was oblivious to day-night cycle.

Язык: Английский

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A single pair of neurons links sleep to memory consolidation in Drosophila melanogaster

eLife, Год журнала: 2015, Номер 4

Опубликована: Янв. 7, 2015

Sleep promotes memory consolidation in humans and many other species, but the physiological anatomical relationships between sleep remain unclear. Here, we show dorsal paired medial (DPM) neurons, which are required for Drosophila, sleep-promoting inhibitory neurons. DPMs increase via release of GABA onto wake-promoting mushroom body (MB) α'/β' Functional imaging demonstrates that DPM activation evokes robust increases chloride MB is unable to cause detectable calcium or cAMP. Downregulation GABAA GABABR3 receptors results loss, suggesting these sleep-relevant targets DPM-mediated inhibition. Regulation by neurons necessary suggests brain processes may be functionally interrelated their shared anatomy. These findings have important implications mechanistic relationship consolidation, arguing a significant role neurotransmission regulating processes.

Язык: Английский

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Courtship behavior in Drosophila melanogaster: towards a ‘courtship connectome’

Current Opinion in Neurobiology, Год журнала: 2012, Номер 23(1), С. 76 - 83

Опубликована: Сен. 28, 2012

The construction of a comprehensive structural, and importantly functional map the network elements connections forming brain represents Holy Grail for research groups working in disparate disciplines. Although technical limitations have restricted mapping human mouse 'connectomes' to level regions, finer degree resolution is attainable fruit fly, Drosophila melanogaster, due armamentarium genetic tools available this model organism. Currently, one most amenable approaches employed by neurobiologists involves neuronal circuitry underlying complex innate behaviors - courtship being classic paradigm. We discuss recent studies aimed at identifying cellular components neural circuits, function these circuits defining causal relationships between activity behavior.

Язык: Английский

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Excitation and inhibition onto central courtship neurons biases Drosophila mate choice

eLife, Год журнала: 2015, Номер 4

Опубликована: Ноя. 14, 2015

The ability to distinguish males from females is essential for productive mate selection and species propagation. Recent studies in Drosophila have identified different classes of contact chemosensory neurons that detect female or male pheromones influence courtship decisions. Here, we examine central neural pathways the brain process using anatomical, calcium imaging, optogenetic, behavioral studies. We find sensory pheromones, but not activate a novel class ventral nerve cord cause activation P1 neurons, male-specific command trigger courtship. In addition, as well those mAL inhibit P1. These demonstrate balance excitatory inhibitory drives onto courtship-promoting controls mating

Язык: Английский

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Propagation of Homeostatic Sleep Signals by Segregated Synaptic Microcircuits of the Drosophila Mushroom Body

Current Biology, Год журнала: 2015, Номер 25(22), С. 2915 - 2927

Опубликована: Окт. 16, 2015

Язык: Английский

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Building a functional connectome of the Drosophila central complex

eLife, Год журнала: 2018, Номер 7

Опубликована: Авг. 20, 2018

The central complex is a highly conserved insect brain region composed of morphologically stereotyped neurons that arborize in distinctively shaped substructures. implicated wide range behaviors and several modeling studies have explored its circuit computations. Most relied on assumptions about connectivity between based their overlap light microscopy images. Here, we present an extensive functional connectome Drosophila melanogaster's at cell-type resolution. Using simultaneous optogenetic stimulation, calcium imaging pharmacology, tested the 70 presynaptic-to-postsynaptic pairs. We identified numerous inputs to complex, but only small number output channels. Additionally, this recurrent appears be sparser than anticipated from Finally, matrix highlights potentially critical role class bottleneck interneurons. All data are provided for interactive exploration website.

Язык: Английский

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Circadian and feeding cues integrate to drive rhythms of physiology in Drosophila insulin-producing cells

Genes & Development, Год журнала: 2016, Номер 30(23), С. 2596 - 2606

Опубликована: Дек. 1, 2016

Circadian clocks regulate much of behavior and physiology, but the mechanisms by which they do so remain poorly understood. While cyclic gene expression is thought to underlie metabolic rhythms, little known about cycles in cellular physiology. We found that Drosophila insulin-producing cells (IPCs), are located pars intercerebralis lack an autonomous circadian clock, functionally connected central clock circuit via DN1 neurons. Insulin mediates output regulating rhythmic a (sxe2) fat body. Patch clamp electrophysiology reveals IPCs display clock-regulated daily rhythms firing event frequency bursting proportion under light:dark conditions. The activity sxe2 additionally regulated feeding, as demonstrated night feeding-induced changes IPC characteristics levels These findings indicate circuit-level regulation metabolism support role for integrating control

Язык: Английский

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